“鄂选1号”山桐子组培繁育体系构建

陈耀兵; 罗凯; 李美东; 黄秀芳; 刘汉蓁; 王水清; 陈圣林

doi:10.12171/j.1000-1522.20210116

摘要:

目的山桐子作为新近开发的木本油料树种，具有广阔的发展前景。“鄂选1号”山桐子作为新近认定的山桐子新品种，具有丰产稳产、适应性强等特点，备受行业关注。高质量“鄂选1号”山桐子良种苗木的需求日益增加，因此亟需构建高效的山桐子良种繁育体系，旨在满足“鄂选1号”山桐子在行业中的广泛运用。

方法（1）以高产优质山桐子良种“鄂选1号”为研究对象，以其新生芽为外植体试验材料，以MS培养基为基础培养基，在对其外植体消毒方法、不定芽诱导培养基激素组合、不定芽增殖培养基激素组合、组培苗生根培养基激素组合优化研究的基础上，利用组织培养技术构建山桐子组培繁育体系。（2）系统地分析了“鄂选1号”外植体的消毒方法及植物激素在其丛芽诱导、增殖及生根过程中的作用。

结果（1）75%乙醇处理30 s，联合0.1% HgCl₂处理8 min是“鄂选1号”山桐子良种外植体最佳消毒方法。（2）不定芽的诱导过程中，（1/2MS + 0.03 mg/L TDZ + 1.5 mg/L 6-BA + 0.05 mg/L NAA）培养基组合适合“鄂选1号”山桐子良种不定芽的诱导。（3）（1/2MS + 0.2 mg/L TDZ + 2.0 mg/L 6-BA + 0.05 mg/L NAA）培养基组合适合“鄂选1号”山桐子良种组培不定芽增殖培养。（4）（1/2MS + 0.3 mg/L NAA + 0.5 mg/L IAA）培养基组合适合山桐子良种组培苗生根诱导，在此条件组培苗生根率达到98%。

结论本研究构建了“鄂选1号”山桐子的快速繁育体系，为“鄂选1号”山桐子的推广应用奠定了基础。

Abstract:

Objective As a newly developed woody oil tree species, Idesia polycarpa has broad development prospects. Idesia polycarpa “Exuan 1”, as a newly identified new variety, has the characteristics of high and stable yield, strong adaptability and so on. The demand for high-quality seedlings of Idesia polycarpa “Exuan 1” is increasing, so it is urgent to build an efficient breeding system to meet the wide application of Idesia polycarpa “Exuan 1” in the industry.

Method Based on the optimization of explant disinfection method, hormone combination of adventitious bud induction medium, hormone combination of adventitious bud proliferation medium and hormone combination of tissue culture seedling rooting medium, tissue culture technology was used to construct a tissue culture and breeding system of Idesia polycarpa “Exuan 1”. In this study, we systematically studied the disinfection methods of the explants of Idesia polycarpa “Exuan 1” and the effects of plant hormones on the process of bud induction, proliferation and rooting.

Result (1) Using 75% ethanol treatment for 30 s combined with 0.1% HgCl₂ treatment for 8 min was the best disinfection method for the explants of Idesia polycarpa “Exuan 1”. (2) 1/2MS + 0.03 mg/L TDZ + 1.5 mg/L 6-BA + 0.05 mg/L NAA medium combination was suitable for adventitious bud induction of Idesia polycarpa “Exuan 1”. (3) The combination of 1/2MS + 0.2 mg/L TDZ + 2.0 mg/L 6-BA + 0.05 mg/L NAA medium was suitable for adventitious bud proliferation of tissue culture of Idesia polycarpa “Exuan 1”. (4) The combination of 1/2MS + 0.3 mg/L NAA + 0.5 mg/L IAA medium was suitable for rooting induction of tissue culture seedlings of Idesia polycarpa “Exuan 1”. Under this condition, the rooting rate of tissue culture seedlings reached 98%.

Conclusion In this study, we construct a rapid propagation system of Idesia polycarpa “Exuan 1”, which lays a foundation for the popularization and application as well as further research on Idesia polycarpa “Exuan 1” at the molecular level.

“鄂选1号”山桐子组培繁育体系构建

Construction of tissue culture breeding system of Idesia polycarpa “Exuan 1”