牡丹遗传作图最适F1分离群体的选择

蔡长福; 刘改秀; 成仿云; 吴静; 钟原; 李敏

doi:10.13332/j.1000-1522.20140134

牡丹遗传作图最适F1分离群体的选择

doi: 10.13332/j.1000-1522.20140134

1.
1 北京林业大学园林学院,国家花卉工程技术研究中心
2.
2 福建省龙岩市林业科学研究所 3 洛阳国家牡丹园

基金项目:

“十二五”国家科技支撑计划课题(2012BAD01B0704)

详细信息

作者简介:
第一作者: 蔡长福。主要研究方向:园林植物遗传育种。Email: caizhf@163.com 地址:100083 北京市清华东路35号北京林业大学园林学院。责任作者: 成仿云,博士,教授。主要研究方向:园林植物资源与育种。Email: chengfy8@263.net 地址:同上。

第一作者: 蔡长福。主要研究方向:园林植物遗传育种。Email: caizhf@163.com 地址:100083 北京市清华东路35号北京林业大学园林学院。责任作者: 成仿云,博士,教授。主要研究方向:园林植物资源与育种。Email: chengfy8@263.net 地址:同上。

Selecting optimal F1 segregation population for genetic linkage mapping in tree peony

1.
1 National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, P. R. China
2.
2 Research Institute of Forestry, Longyan, Fujian, 364000, P. R. China

摘要: 以3株‘凤丹’植株M24、M49、M68为母本,分别以中原牡丹‘红乔’、日本牡丹‘花王’和‘黑龙锦’为父本,采用控制授粉杂交方式,制备了3个规模较大的F1杂交分离群体(个体数量分别为366、233、197)。采用简单重复序列(SSR)标记技术,对这3个分离群体亲本进行多态性检测,结果表明,‘凤丹’M24×‘红乔’分离群体亲本间的多态性水平最高,19对SSR引物共检测到27个多态性位点,亲本间遗传距离为0.707 0;因此,选取了该分离群体作为构建牡丹遗传图谱的作图群体。在此基础上,利用SSR标记技术对作图群体中随机抽取的195株子代个体进行了基因型检测,结果显示19对SSR引物在作图群体中有15对具有多态性,其中13对引物在P0.01水平上符合孟德尔期望分离比,占多态性标记总数的86.7%;测量分析了这195株子代个体的苗高、地径、当年生枝长、复叶长、复叶宽和叶柄长等6个表型性状,结果显示这6个表型性状在作图群体中变异明显,表型值的变异系数均超过15%。综上所述,‘凤丹’M24×‘红乔’F1分离群体适合作为构建牡丹遗传连锁图谱的作图群体。
- 牡丹 /
- SSR /
- 作图群体 /
- 遗传图谱
Abstract: We established three large F1 segregation populations of tree peony (Paeonia Sect. Moutan) through controlled hybridization, using three seeding individuals of P. ostti ‘Fengdan’ as female parents, labeled as M24, M49 and M68, and using P.×suffruticosa Zhongyuan Group ‘Hongqiao’, P.×suffruticosa Japan Group ‘Huawang’ and ‘Hei Longjin’ as male parents, correspondingly. The number of progeny of the three populations is 366, 233 and 197, respectively. We detected the levels of polymorphism between parents of the three segregation populations by employing 19 pairs of simple sequence repeat (SSR) primers. The results showed that, 19 pairs of SSR primers could produce the most informative polymorphism loci of 27 between the ‘Fengdan’ M24 and ‘Hongqiao’ among the parents of three segregation populations. The genetic distance between ‘Fengdan’ M24 and ‘Hongqiao’ was 0.707 0, the farthest among the parents of three segregation populations. Therefore, the segregation population of ‘Fengdan’ M24 × ‘Hongqiao’ was selected for the construction of genetic linkage map in tree peony. On this basis, segregations of genotype in randomly selected 195 progeny from mapping population were investigated using SSR marker. Of 19 pairs of SSR primers, 15 pairs showed the polymorphisms in mapping population, and 13 pairs (86.7%) of them segregated in an expected Mendelian ratio (P0.01). Furthermore, hereditary variations in plant height, ground diameter, current year branch length, compound leaf length, compound leaf width, and petiole length of those 195 progeny were measured and analyzed. The results indicated that the differences of the 6 phenotypic traits of mapping population were significant, and their variation coefficients were all greater than 15%. All above lead to the conclusion that segregation population of ‘Fengdan’ M24 × ‘Hongqiao’ was optimal for the construction of genetic linkage map in tree peony.
- Paeonia Sect. Moutan /
- SSR /
- mapping population /
- genetic linkage map

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牡丹遗传作图最适F1分离群体的选择

doi: 10.13332/j.1000-1522.20140134

Selecting optimal F1 segregation population for genetic linkage mapping in tree peony

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牡丹遗传作图最适F1分离群体的选择

doi: 10.13332/j.1000-1522.20140134

1. 1 北京林业大学园林学院,国家花卉工程技术研究中心

2. 2 福建省龙岩市林业科学研究所 3 洛阳国家牡丹园

English Abstract

Selecting optimal F1 segregation population for genetic linkage mapping in tree peony

1. 1 National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, P. R. China

2. 2 Research Institute of Forestry, Longyan, Fujian, 364000, P. R. China

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留言板

牡丹遗传作图最适F1分离群体的选择

doi: 10.13332/j.1000-1522.20140134

Selecting optimal F1 segregation population for genetic linkage mapping in tree peony

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牡丹遗传作图最适F1分离群体的选择

doi: 10.13332/j.1000-1522.20140134

1. 1 北京林业大学园林学院,国家花卉工程技术研究中心 2. 2 福建省龙岩市林业科学研究所 3 洛阳国家牡丹园

English Abstract

Selecting optimal F1 segregation population for genetic linkage mapping in tree peony

1. 1 National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, P. R. China 2. 2 Research Institute of Forestry, Longyan, Fujian, 364000, P. R. China

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目录

1. 1 北京林业大学园林学院,国家花卉工程技术研究中心

2. 2 福建省龙岩市林业科学研究所 3 洛阳国家牡丹园

1. 1 National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, P. R. China

2. 2 Research Institute of Forestry, Longyan, Fujian, 364000, P. R. China