牡丹遗传作图最适F1分离群体的选择

蔡长福; 刘改秀; 成仿云; 吴静; 钟原; 李敏

doi:10.13332/j.1000-1522.20140134

牡丹遗传作图最适F1分离群体的选择

doi: 10.13332/j.1000-1522.20140134

1.
1 北京林业大学园林学院,国家花卉工程技术研究中心
2.
2 福建省龙岩市林业科学研究所 3 洛阳国家牡丹园

基金项目:

“十二五”国家科技支撑计划课题(2012BAD01B0704)

详细信息

作者简介:
第一作者: 蔡长福。主要研究方向:园林植物遗传育种。Email: caizhf@163.com 地址:100083 北京市清华东路35号北京林业大学园林学院。责任作者: 成仿云,博士,教授。主要研究方向:园林植物资源与育种。Email: chengfy8@263.net 地址:同上。

第一作者: 蔡长福。主要研究方向:园林植物遗传育种。Email: caizhf@163.com 地址:100083 北京市清华东路35号北京林业大学园林学院。责任作者: 成仿云,博士,教授。主要研究方向:园林植物资源与育种。Email: chengfy8@263.net 地址:同上。

Selecting optimal F1 segregation population for genetic linkage mapping in tree peony

1.
1 National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, P. R. China
2.
2 Research Institute of Forestry, Longyan, Fujian, 364000, P. R. China

摘要: 以3株‘凤丹’植株M24、M49、M68为母本,分别以中原牡丹‘红乔’、日本牡丹‘花王’和‘黑龙锦’为父本,采用控制授粉杂交方式,制备了3个规模较大的F1杂交分离群体(个体数量分别为366、233、197)。采用简单重复序列(SSR)标记技术,对这3个分离群体亲本进行多态性检测,结果表明,‘凤丹’M24×‘红乔’分离群体亲本间的多态性水平最高,19对SSR引物共检测到27个多态性位点,亲本间遗传距离为0.707 0;因此,选取了该分离群体作为构建牡丹遗传图谱的作图群体。在此基础上,利用SSR标记技术对作图群体中随机抽取的195株子代个体进行了基因型检测,结果显示19对SSR引物在作图群体中有15对具有多态性,其中13对引物在P0.01水平上符合孟德尔期望分离比,占多态性标记总数的86.7%;测量分析了这195株子代个体的苗高、地径、当年生枝长、复叶长、复叶宽和叶柄长等6个表型性状,结果显示这6个表型性状在作图群体中变异明显,表型值的变异系数均超过15%。综上所述,‘凤丹’M24×‘红乔’F1分离群体适合作为构建牡丹遗传连锁图谱的作图群体。
- 牡丹 /
- SSR /
- 作图群体 /
- 遗传图谱
Abstract: We established three large F1 segregation populations of tree peony (Paeonia Sect. Moutan) through controlled hybridization, using three seeding individuals of P. ostti ‘Fengdan’ as female parents, labeled as M24, M49 and M68, and using P.×suffruticosa Zhongyuan Group ‘Hongqiao’, P.×suffruticosa Japan Group ‘Huawang’ and ‘Hei Longjin’ as male parents, correspondingly. The number of progeny of the three populations is 366, 233 and 197, respectively. We detected the levels of polymorphism between parents of the three segregation populations by employing 19 pairs of simple sequence repeat (SSR) primers. The results showed that, 19 pairs of SSR primers could produce the most informative polymorphism loci of 27 between the ‘Fengdan’ M24 and ‘Hongqiao’ among the parents of three segregation populations. The genetic distance between ‘Fengdan’ M24 and ‘Hongqiao’ was 0.707 0, the farthest among the parents of three segregation populations. Therefore, the segregation population of ‘Fengdan’ M24 × ‘Hongqiao’ was selected for the construction of genetic linkage map in tree peony. On this basis, segregations of genotype in randomly selected 195 progeny from mapping population were investigated using SSR marker. Of 19 pairs of SSR primers, 15 pairs showed the polymorphisms in mapping population, and 13 pairs (86.7%) of them segregated in an expected Mendelian ratio (P0.01). Furthermore, hereditary variations in plant height, ground diameter, current year branch length, compound leaf length, compound leaf width, and petiole length of those 195 progeny were measured and analyzed. The results indicated that the differences of the 6 phenotypic traits of mapping population were significant, and their variation coefficients were all greater than 15%. All above lead to the conclusion that segregation population of ‘Fengdan’ M24 × ‘Hongqiao’ was optimal for the construction of genetic linkage map in tree peony.
- Paeonia Sect. Moutan /
- SSR /
- mapping population /
- genetic linkage map

[1]	CHENG F Y, CHEN D Z. Breeding of new varieties in flare tree peony and varieties classification in tree peonies[J]. Journal of Beijing Forestry University, 1998, 20(2): 27-32.
[2]	CHENG F Y. Advances in the breeding of tree peonies and a cultivar system for the cultivar group[J]. International Journal for Plant Breeding, 2007, 1(2): 89-104.
[3]	XIAO J J. A study on the crossing compatibility and hybrid abortion of Paeonia[D]. Beijing: Beijing Forestry University, 2010.
[4]	ANDERSON J A, CHURCHIL G A, AUTRUQUE J E. Optimizing parental selection for genetic linkage maps[J]. Genome, 1993, 36(1): 181-185.
[5]	WANG Y L. Cross-breeding in tree peony and fertility research of intersectional hybrids[D]. Beijing: Beijing Forestry University, 2009.
[6]	成仿云,陈德忠. 紫斑牡丹新品种选育及牡丹品种分类研究[J]. 北京林业大学学报, 1998, 20(2): 27-32.
[7]	WU J, CHENG F Y, ZHANG D. Utilizing ‘High Noon’ in the crossing breeding of tree peonies and early identification of some hybrids by AFLP markers[J]. Acta Botanica Boreali-Occidentalia Sinica, 2013, 33(8): 1551-1557.
[8]	肖佳佳. 芍药属杂交亲和性及杂种败育研究[D]. 北京: 北京林业大学, 2010.
[9]	王越岚. 牡丹的杂交育种及组间杂种育性的研究[D]. 北京: 北京林业大学, 2009.
[10]	LIU G X, WANG H G, LI M, et al. A study of hybrid seeding of F1 progeny in tree peonies[J]. Modern Agricultural Science and Technology, 2012(22): 155-176.
[11]	吴静,成仿云,张栋. ‘正午’牡丹的杂交利用及部分杂种AFLP鉴定[J]. 西北植物学报, 2013, 33(8): 1551-1557.
[12]	PANG L Z, CHENG F Y, ZHONG Y, et al. Phenotypic analysis of association population for flare tree peony[J]. Journal of Beijing Forestry University, 2012, 34(6): 115-120.
[13]	XU Y B, ZHU L H. Molecular quantitative genetics[M]. Beijing: China Agriculture Press, 1994: 291.
[14]	刘改秀,王海歌,李敏,等. 牡丹杂种F1代育苗研究[J]. 现代农业科技, 2012(22): 155-176.
[15]	庞利铮,成仿云,钟原,等. 紫斑牡丹关联分析群体的表型分析[J]. 北京林业大学学报, 2012, 34(6): 115-120.
[16]	SU M H. Breeding technology of Paeonia suffruticosa and analysis of genetic diversity of F1 progenies of Paeonia suffruticosa[D]. Tai'an: Shandong Agricultural University, 2013.
[17]	HOU X G, GUO D L, CHENG S P, et al. Development of thirty new polymorphic microsatellite primers for Paeonia suffruticosa[J]. Biologia Plantarum, 2011, 55(4): 708-710.
[18]	HAN X, CHENG F Y, XIAO J J, et al. Crosses of Paeonia ostii ‘Feng Dan Bai’ as maternal parents and an analysis on the potential in tree peony breeding[J]. Journal of Beijing Forestry University, 2014, 36(4): 121-125.
[19]	HOMOLKA A, BERENYI M, BURG K, et al. Microsatellite markers in the tree peony,Paeonia suffruticosa (Paeoniaceae)[J]. American Journal of Botany, 2010, 97(6): e42-e44.
[20]	MIAO M J, LI C Q, SI J. SSR markers and their application in horticulture plant breeding[J]. Journal of Changjiang Vegetables, 2010(10): 1-5.
[21]	ZHANG D Q, ZHANG Z Y, SONG W, et al. Optimizing segregation population selection for genetic linkage maps in Populus tomentosa[J]. Journal of Beijing Forestry University, 2003, 25(4): 21-24.
[22]	WANG J X, XIA T, ZHANG J M, et al. Isolation and characterization of fourteen microsatellites from a tree peony (Paeonia suffruticosa)[J]. Conservation Genetics, 2009, 10(4): 1029-1031.
[23]	YU H P, CHENG F Y, ZHONG Y, et al. Development of simple sequence repeat (SSR) markers from Paeonia ostii to study the genetic relationships among tree peonies (Paeoniaceae)[J]. Scientia Horticulturae, 2013, 164: 58-64.
[24]	LIU Y, CAI M, HE D, et al. Construction of F1 segregation population for genetic linkage maps in Lagerstroemia[J]. Journal of Northeast Forestry University, 2013, 41(9): 72-75.
[25]	WANG X Q. Studies on genetic diversity of Paeonia delavayi in Shangri-la[D]. Beijing: Beijing Forestry University, 2009.
[26]	LIU K J, MUSE S V. PowerMarker: an integrated analysis environment for genetic marker analysis[J]. Bioinformatics, 2005, 21(9): 2128-2129.
[27]	LI Z Y, ZHANG H Y. Morphological variation and diversity in populations of Paeonia lutea[J]. Journal of Northwest Forestry University, 2011, 26(4): 117-122.
[28]	VAN OOIJEN J W. JoinMap 4: software for the calculation of genetic linkage maps in experimental populations[DB/CD]. Wageningen: Plant Research International, 2006.
[29]	HELENTJARIS T. A genetic linkage map for maize based on RFLPs[J]. Trends in Genetics, 1987, 3: 217-221.
[30]	LI Y, WANG D W, LI Z Q, et al. Establishment of mapping population in Eucammia ulmoides[J]. Journal of Northwest Forestry University, 2012, 27(2): 62-65.
[31]	HE D, TANG W, LIU Y, et al. Linkage analysis of phonotypic traits of Lagerstroemia caudata and L. indica F1 population uing SSR markers[J]. Journal of Beijing Forestry University, 2012, 34(6): 121-125.
[32]	徐云碧,朱立煌. 分子数量遗传学[M]. 北京: 中国农业出版社, 1994: 291.
[33]	YUAN J H, CORNILLE A, GIRAUD T, et al. Independent domestications of cultivated tree peonies from different wild peony species[J]. Molecular Ecology, 2014, 23(1): 82-95.
[34]	苏美和. 牡丹育种技术及杂交一代遗传多样性的研究[D]. 泰安: 山东农业大学, 2013.
[35]	韩欣,成仿云,肖佳佳,等. 以‘凤丹白’为母本的杂交及其育种潜力分析[J]. 北京林业大学学报, 2014, 36(4): 121-125.
[36]	苗明军,李成琼,司军. SSR标记及其在园艺植物育种中的应用[J]. 长江蔬菜, 2010(10): 1-5.
[37]	MOGHADDAMH H, LEUS L, DE RIEK J, et al. Construction of a genetic linkage map with SSR, AFLP and morphological markers to locate QTLs controlling pathotype-specific powdery mildew resistance in diploid roses[J]. Euphytica, 2012, 184(3): 413-427.
[38]	SUN L D, YANG W R, ZHANG Q X, et al. Genome-wide characterization and linkage mapping of simple sequence repeats in mei (Prunus mume Sieb. et Zucc.)[J]. PLoS ONE, 2013, 8(3): e59562.
[39]	YAGI M, YAMAMOTO T, ISOBE S, et al. Identification of tightly linked SSR markers for flower type in carnation (Dianthus caryophyllus L.)[J]. Euphytica, 2014, 198(2): 175-183.
[40]	YAGI M, YAMAMOTO T, ISOBE S, et al. Construction of a reference genetic linkage map for carnation (Dianthus caryophyllus L.)[J]. BMC Genomics, 2013, 14(1): 734.
[41]	GAI S P, ZHANG Y X, MU P, et al. Transcriptome analysis of tree peony during chilling requirement fulfillment:assembling, annotation and markers discovering[J]. Gene, 2012, 497(2): 256-262.
[42]	ZHANG J J, SHU Q Y, LIU Z A, et al. Two EST-derived marker systems for cultivar identification in tree peony[J]. Plant Cell Reports, 2012, 31(2): 299-310.
[43]	张德强,张志毅,宋婉,等. 毛白杨遗传作图最适分离群体的选择[J]. 北京林业大学学报, 2003, 25(4): 21-24.
[44]	ZHANG D Q, ZHANG Z Y, YANG K, et al. Genetic mapping in (Populus tomentosa× Populus bolleana) and P. tomentosa Carr. using AFLP markers[J]. Theoretical and Applied Genetics, 2004, 108(4): 657-662.
[45]	ZHANG F, CHEN S, CHEN F, et al. SRAP-based mapping and QTL detection for inflorescence-related traits in chrysanthemum (Dendranthema morifolium)[J]. Molecular Breeding, 2011, 27(1): 11-23.
[46]	HIBRAND-SAINT OYANT L, CRESPEL L, RAJAPAKSE S, et al. Genetic linkage maps of rose constructed with new microsatellite markers and locating QTL controlling flowering traits[J]. Tree Genetics Genomes, 2007, 4(1): 11-23.
[47]	HE D, LIU Y, CAI M, et al. The first genetic linkage map of crape myrtle (Lagerstroemia) based on amplification fragment length polymorphisms and simple sequence repeats markers[J]. Plant Breeding, 2014, 133(1): 138-144.
[48]	刘阳,蔡明,贺丹,等. 紫薇遗传作图F1分离群体的选择[J]. 东北林业大学学报, 2013, 41(9): 72-75.
[49]	王晓琴. 香格里拉滇牡丹遗传多样性研究[D]. 北京: 北京林业大学, 2009.
[50]	李宗艳,张海燕. 黄牡丹表型变异及多样性研究[J]. 西北林学院学报, 2011, 26(4): 117-122.
[51]	李煜,王大玮,李周岐,等. 杜仲遗传作图群体的建立[J]. 西北林学院学报, 2012, 27(2): 62-65.
[52]	贺丹,唐婉,刘阳,等. 尾叶紫薇与紫薇F1代群体主要表型性状与SSR标记的连锁分析[J]. 北京林业大学学报, 2012, 34(6): 121-125.

[1]	毛秀红, 朱士利, 李善文, 华辉, 田书勇, 仲伟国, 董玉峰, 安新民. 基于荧光SSR标记的毛白杨核心种质构建 . 北京林业大学学报, 2020, 42(7): 40-47. doi: 10.12171/j.1000-1522.20190413
[2]	吴钰滢, 周璇, 徐庭亮, 常征, 易星湾, 高华北, 赵红霞, 王佳, 程堂仁, 张启翔, 潘会堂. 现代月季品种‘赞歌’和粉团蔷薇杂交后代鉴定与评价 . 北京林业大学学报, 2019, 41(3): 124-133. doi: 10.13332/j.1000-1522.20180255
[3]	洪文娟, 郝兆祥, 刘康佳, 罗华, 毕润霞, 苑兆和, 宗世祥, 王君. 基于石榴全基因组序列的SSR标记开发及鉴定 . 北京林业大学学报, 2019, 41(8): 38-47. doi: 10.13332/j.1000-1522.20190167
[4]	潘丽芹, 李纪元, 李绍翠, 范正琪, 殷恒福, 何丽波. 基于山茶转录组的SSR标记开发及亲缘关系分析 . 北京林业大学学报, 2019, 41(7): 111-120. doi: 10.13332/j.1000-1522.20190101
[5]	杨晨阳, 于超, 马玉杰, 罗乐, 潘会堂, 张启翔. 基于SSR标记和单拷贝核基因的蔷薇属植物系统发生分析 . 北京林业大学学报, 2018, 40(12): 85-96. doi: 10.13332/j.1000-1522.20180088
[6]	修宇, 吴国栋, 陈德忠, 赵孝庆, 唐文思, 王华芳. 牡丹绿化油用品种繁殖栽培技术 . 北京林业大学学报, 2017, 39(1): 112-118. doi: 10.13332/j.1000-1522.20160045
[7]	刘建鑫, 杨柳慧, 魏冬霞, 于晓南. 芍药属组内组间杂交及部分后代核型分析与SSR鉴定 . 北京林业大学学报, 2017, 39(4): 72-78. doi: 10.13332/j.1000-1522.20160181
[8]	刘建鑫, 于晓南. 芍药与牡丹远缘杂交花粉萌发与花粉管生长行为观察 . 北京林业大学学报, 2016, 38(9): 80-86. doi: 10.13332/j.1000-1522.20150439
[9]	程祥, 张梅, 毛建丰, 钮世辉, 马俊, 李伟, 李悦. 有限种群油松种子园的遗传多样性与交配系统 . 北京林业大学学报, 2016, 38(9): 8-15. doi: 10.13332/j.1000-1522.20150486
[10]	韩欣, 成仿云, 肖佳佳, 王越岚, 张栋, 王莹, 钟原. 以凤丹白'为母本的杂交及其育种潜力分析 . 北京林业大学学报, 2014, 36(4): 121-125. doi: 10.13332/j.cnki.jbfu.2014.04.022
[11]	丁晓六, 刘佳, 赵红霞, 王晶, 罗乐, 潘会堂, 张启翔. 现代月季和玫瑰杂交后代的鉴定与评价 . 北京林业大学学报, 2014, 36(5): 123-130. doi: 10.13332/j.cnki.jbfu.2014.05.005
[12]	陈凌娜, 马庆国, 张俊佩, 周贝贝, 裴东. 核桃BES-SSR 的开发及在遗传多样性分析中的应用 . 北京林业大学学报, 2014, 36(6): 24-29. doi: 10.13332/j.cnki.jbfu.2014.06.008
[13]	高平, 刘玉英, 成仿云, 刘改秀, 李敏. 基于专用目标的中原牡丹品种评价与筛选 . 北京林业大学学报, 2013, 35(4): 106-111.
[14]	范付华, 杨勇胜, 李庆宏, 邓勇, 文晓鹏. 利用ISSR分析技术鉴定贵州枇杷新种质的研究 . 北京林业大学学报, 2012, 34(4): 52-57.
[15]	秦英英, 韩海荣, 康峰峰, 赵琦. 基于SSR 标记的山西省辽东栎自然居群遗传多样性分析 . 北京林业大学学报, 2012, 34(2): 61-65.
[16]	贺丹, 唐婉, 刘阳, 蔡明, 潘会堂, 张启翔. 尾叶紫薇与紫薇F1代群体主要表型性状与SSR标记的连锁分析 . 北京林业大学学报, 2012, 34(6): 121-125.
[17]	吴根松, 孙丽丹, 张启翔, 潘会堂, 蔡明. 基于近缘物种SSR引物和EST-SSR序列的梅花SSR引物开发 . 北京林业大学学报, 2011, 33(5): 103-108.
[18]	李萍, 成仿云, 张颖星. 防褐剂对牡丹组培褐化发生、组培苗生长和增殖的作用 . 北京林业大学学报, 2008, 30(2): 71-76.
[19]	徐向舟, 周成理, 王清奎, 王顺忠, 雷加富, 耿玉清, 徐基良, 王旭, 宋颖琦, 李瑞, 姚洪军, 马尔妮, 任琴, 何亚平, 齐实, 王尚德, 白新祥, 刘秀萍, 惠刚盈, 余雁, 武广涛, 崔国发, 费本华, 张克斌, 徐海, 刘大庆, 石玉杰, 杨莉, 杨谦, 费世民, 汪思龙, 陈丽华, 俞国胜, 王飞, 康向阳, 云琦, 史军义, 周国逸, 赵广杰, 白翠霞, 胡可, 戴思兰, 李代丽, 张恒明, 赵铁蕊, 宋维峰, 胡艳波, 王百田, 李忠, 孙阁, 张红武, 徐秉玖, 秦跟基, 冯宗炜, 孙阁, 张波, 胡永建, 陈晓鸣, 蒋俊明, 高荣孚, 易传辉, 杨晓晖, 代力民, 张德强, 朱金兆, 王戈, 瞿礼嘉, 陈秀明, 王亮生, 董占地, 陈华君, 张慧, 王树森, 陈峻崎, 王庆礼, 朱明东, 石雷, 乔锋, 肖玉保, 金幼菊, 余英, 武波, 闫俊华, 陈晓阳, 杨海龙, 杨俊杰, 唐森强, 赵辉, 李镇宇, 杨莉, SteveMcNulty. 牡丹矮化品种亲缘关系的AFLP分析 . 北京林业大学学报, 2006, 28(5): 73-77.
[20]	盖颖, 王盛萍, 李云成, 王岩, 贺庆棠, 张金凤, 罗菊春, 冶民生, 蒋佳荔, 侯旭, 谢响明, 何磊, 张文娟, 张学俭, 高鹏, 李绍才, 朱妍, 孙宇瑞, 李永慈, 柳新伟, 李吉跃, 何静, 马道坤, 吴玉英, 吕建雄, 冯仲科, 成仿云, 崔保山, 康向阳, 张志强, 王文棋, 廖学品, 关文彬, 昌明, 孙海龙, 申卫军, 陆佩玲, 张华丽, 唐守正, 张桂莲, 关毓秀, 路婷, 赵广杰, 于晓南, 何权, 王军辉, 石碧, 静洁, 孙阁, 史剑波, 李小飞, 吴斌, 张平冬, 蒋湘宁, 杨志荣, 赵燕东, 蒲俊文, 孙晓霞, 彭少麟, 陈永国, 马克明, 张满良, 汪燕, 王尚德, 胡文忠, 余新晓, 刘国华, 林威, 汪西林. 赤霉素和生根粉对牡丹促成栽培影响的初步研究 . 北京林业大学学报, 2006, 28(1): 84-87.

点击查看大图

计量

文章访问数: 971
HTML全文浏览量: 112
PDF下载量: 10
被引次数: 0

姓名
邮箱
手机号码
标题
留言内容
验证码

留言板

牡丹遗传作图最适F1分离群体的选择

doi: 10.13332/j.1000-1522.20140134

Selecting optimal F1 segregation population for genetic linkage mapping in tree peony

计量

牡丹遗传作图最适F1分离群体的选择

doi: 10.13332/j.1000-1522.20140134

1. 1 北京林业大学园林学院,国家花卉工程技术研究中心

2. 2 福建省龙岩市林业科学研究所 3 洛阳国家牡丹园

English Abstract

Selecting optimal F1 segregation population for genetic linkage mapping in tree peony

1. 1 National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, P. R. China

2. 2 Research Institute of Forestry, Longyan, Fujian, 364000, P. R. China

全文HTML

目录

留言板

牡丹遗传作图最适F1分离群体的选择

doi: 10.13332/j.1000-1522.20140134

Selecting optimal F1 segregation population for genetic linkage mapping in tree peony

计量

出版历程

牡丹遗传作图最适F1分离群体的选择

doi: 10.13332/j.1000-1522.20140134

1. 1 北京林业大学园林学院,国家花卉工程技术研究中心 2. 2 福建省龙岩市林业科学研究所 3 洛阳国家牡丹园

English Abstract

Selecting optimal F1 segregation population for genetic linkage mapping in tree peony

1. 1 National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, P. R. China 2. 2 Research Institute of Forestry, Longyan, Fujian, 364000, P. R. China

全文HTML

目录

1. 1 北京林业大学园林学院,国家花卉工程技术研究中心

2. 2 福建省龙岩市林业科学研究所 3 洛阳国家牡丹园

1. 1 National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, P. R. China

2. 2 Research Institute of Forestry, Longyan, Fujian, 364000, P. R. China