紫丁香多酚提取工艺优化及抗氧化活性的研究

赵青; 吕肖斐; 朱晓冉; 张娜; 郭庆启

doi:10.13332/j.1000-1522.20140174

紫丁香多酚提取工艺优化及抗氧化活性的研究

doi: 10.13332/j.1000-1522.20140174

1.
1 东北林业大学林学院;
2.
2 哈尔滨商业大学食品工程学院

基金项目:

教育部东北林业大学大学生创新训练计划项目(201310225093)、东北林业大学卓越农林人才教育培训计划改革试点项目(41110211)、黑龙江省应用技术研究与开发计划项目(GC13B215)。

详细信息

作者简介:
赵青。主要研究方向:食用植物资源。Email: 15642766453@qq.com 地址:150040黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院。责任作者: 郭庆启,博士,讲师。主要研究方向:天然产物化学。Email:qingqiguo@nefu.edu.cn 地址同上。

赵青。主要研究方向:食用植物资源。Email: 15642766453@qq.com 地址:150040黑龙江省哈尔滨市香坊区和兴路26号东北林业大学林学院。责任作者: 郭庆启,博士,讲师。主要研究方向:天然产物化学。Email:qingqiguo@nefu.edu.cn 地址同上。

Optimization of extraction technology for lilac polyphenols and their antioxidant activity.

1.
1School of Forestry, Northeast Forestry University, Harbin, Heilongjiang, 150040, P.R. China;
2.
2 College of Food Engineering, Harbin University of Commerce, Harbin, Heilongjiang, 150086, P.R. China.

摘要: 以紫丁香为原料,对紫丁香多酚的提取及抗氧化活性进行了研究,得到最适提取工艺条件为:乙醇浓度55%、料液比1∶50、浸提温度70 ℃、浸提时间50 min,此工艺条件下紫丁香多酚的得率为(1.73±0.05)mg/g。采用生物活性追踪法研究发现紫丁香多酚中抗氧化活性最强组分的极性为弱极性,当这部分样液质量浓度为70 μg/mL时,总还原能力为0.64±0.01,DPPH自由基的清除能力为(79.44±0.67)%。研究发现经人工胃液处理后,紫丁香多酚抗氧化活性最强组分的抗氧化能力上升,而经人工肠液处理后其抗氧化能力下降。
- 紫丁香 /
- 多酚 /
- 提取 /
- 抗氧化活性
Abstract: We extracted polyphenols from lilac (Syringa oblate) and tested their antioxidant activity. The optimal extraction conditions were found to be ethanol concentration of 55%, material/liquid ratio of 1∶50, extraction temperature of 70 ℃ and extraction time of 50 min. Under these conditions, the yield of lilac polyphenols was (1.73±0.05) mg/g. With the biological activity tracking method it was found that the polarity of the strongest antioxidant fraction in lilac polyphenols was weak, the total reducing capacity was 0.64±0.01 and DPPH free radical scavenging capacity was (79.44±0.67)% when the concentration of the sample solution was 70 μg/mL. The study revealed that the capacity of the strongest antioxidant fraction of lilac polyphenols was increased after the treatment by artificial gastric solution, while decreased after the treatment by artificial intestinal solution.
- lilac /
- polyphenol /
- extraction /
- antioxidation activity

[1]	WANG L J. Technology for extracting chlorophyll from leaves of Syringa oblata L. by ultrasonic-assisted method[J]. Transactions of the CSAE, 2009,25(Suppl.1): 171-174.
[2]	王立娟. 超声波辅助提取紫丁香叶叶绿素的工艺[J]. 农业工程学报,2009,25(增刊1):171-174.
[3]	HUI R H, LI T C, HOU D Y. Analysis of volatile components from flower and leaf of Syringa oblate Lindl. by GC/MS[J]. Journal of Chinese Mass Spectrometry Society, 2002,23(4): 210-213.
[4]	回瑞华,李铁纯,侯冬岩. GC/MS分析紫丁香花与叶中的挥发性化学成分[J]. 质谱学报,2002,23(4):210-213.
[5]	WANG D D, LIU S Q, CHEN Y J, et al. Studies on the active constituents of Syringa oblata Lindl [J]. Acta Pharmaceutica Sinica, 1982,17(12): 951-952.
[6]	王丹丹,刘盛泉,陈英杰,等. 紫丁香有效成分的研究[J]. 药学学报,1982,17(12):951-952.
[7]	陈文学,豆海港,仇厚援. 丁香提取物抗氧化研究[J]. 食品研究与开发,2006,27(1):149-152.
[8]	CHEN W X, DOU H G, QIU H Y. Research of clove extraction antioxidant properties[J]. Journal of Food Research and Development, 2006,27(1): 149-152.
[9]	WEI Z J. Studies on the chemical constituents of Syringa oblate Lindl. from the leaf and its pharmacological activities[D]. Changchun:Jilin University, 2004.
[10]	MISHRA R K,SINGH S K. Safety assessment of Syzygium aromaticum flower bud (clove) function in mice[J]. Food and Chemical Toxicology,2008,46(10):3333-3338.
[11]	RODRIGUES T G,FEMANDES A,SOUSA J P B, et al. In vitro and in vivo effects of clove on pro-inflammatory cytokines production by macrophages[J]. Natural Product Research, 2009,23(4):319-326.
[12]	CAI E B, ZHANG C X, ZHU Q G,et al. Optimization of extraction process based on central composite design and response surface methodology for syringing in the peels of Syringa reticulate[J]. Chin J Pharm Anal,2011,31(9):1808-1811.
[13]	JIN Y, SUN A D. Studies on the structure and biological activity of plant polyphenols[J]. Food and Nutrition in China, 2005(9): 27-29.
[14]	SINGH A K,DHAMANIGI S S,ASAD M. Anti-stress activity of hydro-alcoholic extract of Eugenia caryophyllus buds(clove)[J]. India Journal of Pharmacology,2009,41(1):28-31.
[15]	魏征骥. 紫丁香化学成分及其生物活性的研究[D]. 长春:吉林大学,2004.
[16]	JIANG S H, WANG S Y, MA H L,et al. Extracting technology and antioxidant activity of bioactive components from clove[J]. Transactions of the Chinese Society for Agriculture Machinery, 2010,41(1): 132-138.
[17]	蔡恩博,张崇禧,朱启光,等. 星点设计:效应面法优选暴马丁香树皮中紫丁香苷的提取工艺[J]. 药物分析杂志,2011,31(9):1808-1811.
[18]	GB/T8313 Determination of total polyphenols and catechins content in tea[S]. Beijing: Standards Press of Press, 2008.
[19]	金莹,孙爱东. 植物多酚的结构及生物学活性的研究[J]. 中国食物与营养,2005(9):27-29.
[20]	GUO Q Q, ZHANG N, LI M Y, et al. Relationship between polyphenols and antioxidation in larch pine cones and altitude[J]. Journal of Beijing Forestry University, 2013,35(1): 59-63.
[21]	GUO Q Q, ZHANG N, LIU Y B, et al. Microwave and ultrasonic effects on the extraction and antioxidation of larch bark polyphenols[J]. Journal of Beijing Forestry University, 2015,37(1): 134-138.
[22]	KUMAR R,TAYADE A,CHAURASIA O P,et al. Evaluation of anti-oxidant activities and total phenol and flavonoid content of the hydro-alcoholic extracts of Rhodiola sp [J]. Pharmacognosy Journal,2010,2(11):431-435.
[23]	SU X Y, WANG Z Y. Polyphenol extraction from Pinus koraiensis seed putamina and its antioxidant activities[J]. Transactions of the CSAE, 2009,25(Suppl.1): 198-203.
[24]	LAVINIA A,GIANCARLO C,LUCA G,et al. Ultrasound-assisted extraction of clove buds using batch-and flow-reactors: a comparative study on a pilot scale[J]. Innovative Food Science and Emerging Technologies,2013, 20:167-172.
[25]	GUO Q Q, ZHANG N, LI M Y,et al. Relationship between polyphenols and antioxidation in larch pine cones and growing slope aspect[J]. Journal of Beijing Forestry University, 2014,36(1): 62-65.
[26]	江慎华,王书源,马海乐,等. 丁香活性物质提取工艺优化与抗氧化活性研究[J]. 农业机械学报,2010,41(1):132-138.
[27]	JIANG S H,WANG C L,CHEN Z Q, et al. Antioxidants properties of the extract and subfractions from old leaves of Toona sinensisroem(Meliaceae)[J]. Journal of Food Biochemistry,2009,33(3):425-441.
[28]	Chinese Pharmacopoeia Commission. Pharmacopoeia of People's Republic China 2010 edition:2[M]. Beijing: China Medical Science and Technology Press, 2010.
[29]	GB/T8313茶叶中茶多酚和儿茶素类含量的检测方法[S].北京:中国标准出版社,2008.
[30]	WANG L L. Extraction of polyphenols from leaves of Syringa oblate Lindl. and evaluation of their antioxidative property[D].Harbin: Northeast Agriculture University,2007.
[31]	LI J J, HUANG K Y, GONG J L, et al. Study on microwave extraction of total flavonoids from Cinnamomum wilsonii leaves[J]. Journal of Food Research and Development, 2007,28(8): 71-75.
[32]	郭庆启,张娜,李梦云,等. 落叶松松塔成分和抗氧化能力与海拔的相关性研究[J]. 北京林业大学学报,2013,35(1):59-63.
[33]	ZHOU Y, DU X F, JIANG S H,et al. Comparison of ultrasound and microwave-assisted extractions of bioactive components from lotus leaf and effect of the artificial gastric juice immersion on the antioxidant activities[J]. Science and Technology of Food Industry, 2012,33(17): 106-113.
[34]	郭庆启,张娜,刘奕兵,等. 微波和超声波对落叶松树皮多酚浸提及其抗氧化效果影响[J]. 北京林业大学学报,2015,37(1):134-138.
[35]	JIANG S H, CAI Z P, LIAO L,et al. Extraction of antioxidants from clove and effect of artificial gastrointestinal juice immersion on its antioxidant properties[J]. Transactions of the Chinese Society for Agriculture Machinery, 2012,43(7): 149-154.
[36]	苏晓雨,王振宇. 红松种子壳多酚物质的提取及抗氧化特性[J]. 农业工程学报,2009,25(增刊1):198-203.
[37]	郭庆启,张娜,李梦云,等. 落叶松松塔多酚含量、抗氧化能力与生长坡向的相关性[J]. 北京林业大学学报,2014,36(1):62-65.
[38]	国家药典委员会. 中华人民共和国药典:2部[M]. 北京:中国医药科技出版社,2010.
[39]	MADUREIRA A R,AMORIM M,GOMES A M,et al. Protective effect of whey cheese matrix on probiotic strains exposed to simulated gastrointestinal conditions[J]. Food Research International,2011,44(1):465-470.
[40]	王丽丽. 紫丁香叶多酚物质的提取及抗氧化性能研究[D]. 哈尔滨:东北农业大学,2007.
[41]	李娇娟,黄克瀛,龚建良,等. 微波法提取川桂叶总黄酮工艺条件的研究[J]. 食品研究与开发,2007,28(8):71-75.
[42]	周勇,杜先锋,江慎华,等. 荷叶活性物质超声、微波辅助提取比较及人工胃液处理对其抗氧化活性的影响[J]. 食品工业科技,2012,33(17):106-113.
[43]	江慎华,蔡志鹏,廖亮,等. 丁香抗氧化活性物质提取及人工胃肠液对其活性的影响[J]. 农业机械学报,2012,43(7):149-154.

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紫丁香多酚提取工艺优化及抗氧化活性的研究

doi: 10.13332/j.1000-1522.20140174

Optimization of extraction technology for lilac polyphenols and their antioxidant activity.

计量

紫丁香多酚提取工艺优化及抗氧化活性的研究

doi: 10.13332/j.1000-1522.20140174

1. 1 东北林业大学林学院;

2. 2 哈尔滨商业大学食品工程学院

English Abstract

Optimization of extraction technology for lilac polyphenols and their antioxidant activity.

1. 1School of Forestry, Northeast Forestry University, Harbin, Heilongjiang, 150040, P.R. China;

2. 2 College of Food Engineering, Harbin University of Commerce, Harbin, Heilongjiang, 150086, P.R. China.

全文HTML

目录

留言板

紫丁香多酚提取工艺优化及抗氧化活性的研究

doi: 10.13332/j.1000-1522.20140174

Optimization of extraction technology for lilac polyphenols and their antioxidant activity.

计量

出版历程

紫丁香多酚提取工艺优化及抗氧化活性的研究

doi: 10.13332/j.1000-1522.20140174

1. 1 东北林业大学林学院; 2. 2 哈尔滨商业大学食品工程学院

English Abstract

Optimization of extraction technology for lilac polyphenols and their antioxidant activity.

1. 1School of Forestry, Northeast Forestry University, Harbin, Heilongjiang, 150040, P.R. China; 2. 2 College of Food Engineering, Harbin University of Commerce, Harbin, Heilongjiang, 150086, P.R. China.

全文HTML

目录

1. 1 东北林业大学林学院;

2. 2 哈尔滨商业大学食品工程学院

1. 1School of Forestry, Northeast Forestry University, Harbin, Heilongjiang, 150040, P.R. China;

2. 2 College of Food Engineering, Harbin University of Commerce, Harbin, Heilongjiang, 150086, P.R. China.