Abstract: In this study, ethanol was prepared by fermentation of immobilized yeast, with enzymatic hydrolysate of Tara residues as raw material, and the process was optimized by single factor and orthogonal experiments. The optimal experiment conditions were the concentration of substrate sugar 30%, dosage of yeast 1.5 g, and mass ratio of sodium alginate to yeast 1∶2.0. The impact of various factors on ethanol yield was ranked from high to low as: the mass ratio of sodium alginate to yeast concentration of substrate sugar yeast dosage. Under the optimum conditions, the ethanol yield was 76.74% and utilization of substrate sugar was 97.18%. Taking into account the cost and yield of ethanol production, the immobilized yeast was reused three times.