With alkali lignin and PVA as raw material, glyoxal as crosslinker,the alkali lignin-PVA crosslinked blend slow-release pesticide film was prepared by tape casting. The chemical structure of the film was analyzed by FT-IR. The gas permeability and mechanical properties were characterized. The results showed that alkali lignin had been crosslinked with PVA successfully. Compared with non-acetaniprid crosslinked film, oxygen transmission of the acetaniprid slow-release film increased, its carbon dioxide transmission reduced, and its tensile strength and elongation at break decreased. The optimum preparation conditions of acetaniprid slow-release film were the mass ratio of alkali lignin and PVA 3∶7, glyoxal dosage 1.5 mL, acetaniprid dosage 0.5 g, and the release amount of acetaniprid 31.8%. The water solving method indicated that there is certain effect of potion proportion and temperature on the cumulative release rate of acetaniprid-loaded film.