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    刘剑锋, 张彧楚, 刘挺, 塞拉尔·唐瑟, 程云清. 榛实象甲高致病力菌株筛选及侵染过程的显微观察[J]. 北京林业大学学报, 2017, 39(3): 32-37. DOI: 10.13332/j.1000-1522.20160322
    引用本文: 刘剑锋, 张彧楚, 刘挺, 塞拉尔·唐瑟, 程云清. 榛实象甲高致病力菌株筛选及侵染过程的显微观察[J]. 北京林业大学学报, 2017, 39(3): 32-37. DOI: 10.13332/j.1000-1522.20160322
    LIU Jian-feng, ZHANG Yu-chu, LIU Ting, Celal Tuncer, CHENG Yun-qing. Screening of a highly pathogenic strain against hazelnut weevil and microscopic observation on its infection process[J]. Journal of Beijing Forestry University, 2017, 39(3): 32-37. DOI: 10.13332/j.1000-1522.20160322
    Citation: LIU Jian-feng, ZHANG Yu-chu, LIU Ting, Celal Tuncer, CHENG Yun-qing. Screening of a highly pathogenic strain against hazelnut weevil and microscopic observation on its infection process[J]. Journal of Beijing Forestry University, 2017, 39(3): 32-37. DOI: 10.13332/j.1000-1522.20160322

    榛实象甲高致病力菌株筛选及侵染过程的显微观察

    Screening of a highly pathogenic strain against hazelnut weevil and microscopic observation on its infection process

    • 摘要: 榛实象甲是目前我国榛园中导致产量损失的首要害虫,生产中以化学防治为主,易导致果品化学残留超标问题。本研究的目的在于筛选榛实象甲的高毒力菌株,以期为其生物学防治提供科学依据。本研究使用2株绿僵菌(CGMCC No.3.7986、3.4607)、1株白僵菌(CGMCC No. 12108)接种榛实象甲成虫,检测其侵染榛实象甲累积死亡率随时间的变化,并用解剖镜与扫描电镜观察了白僵菌侵染榛实象甲成虫的过程。结果表明,白僵菌菌株12108侵染榛实象甲后,在处理后的第4天与第6天累积死亡率分别达到91.67%和100%,远高于同期绿僵菌3.7986的6.67%和17.5%及绿僵菌3.4607的10.0%和22.5%;白僵菌菌株12108的LT50和LT90值分别为2.56和4.42d,远小于绿僵菌3.7986的11.40和17.70d及绿僵菌3.4607的8.80和12.80d;在侵染开始时,白僵菌菌株12108一般从口器、触角、胸足基部、胸足关节等有缝隙的部位开始生长,接种后10d时,该虫表面被浓密的菌丝所覆盖,胸腔内部也充满菌丝,体表开始形成大量分生孢子。以上研究结果确认白僵菌菌株12108对榛实象甲有强致病力,这为榛实象甲生物农药的开发提供了重要依据。

       

      Abstract: Hazelnut weevil is the primary pest causing yield loss of hazelnut orchard in China. In practice, chemical spray is the major method of controlling hazelnut weevil. However, frequent chemical spray may cause excessive residual of hazelnut products. The aim of present study is to screen a highly pathogenic strain against nut weevil and provide scientific evidence for its biocontrol. Metarhizium anisopliae var. acridum 3.7986, M. anisopliae 3.4607 and Beauveria bassiana subsp. palomenae (CGMCC No. 12108) were used to infect nut weevil adults, and the time course of accumulated lethal ratio of nut weevil adults was analyzed after inoculation. It was shown that, after inoculated with B. bassiana 12108, the accumulated lethal ratio of nut weevil adults reached 91.67% and 100% on the fourth and sixth day, respectively, which was much higher than in inoculated with M. anisopliae 3.4607 (6.67% and 17.5%), and M. anisopliae 3.4607 (10.0% and 22.5%). LT50 and LT90 of B.bassiana 12108 were 2.56 and 4.42 days, respectively, which were much shorter than that of M. anisopliae 3.7986 (11.40 and 17.70 days) and M. anisopliae 3.4607 (8.80 and 12.80 days). Moreover, the process of B. bassiana infecting nut weevil adults was observed using dissecting microscope and scanning electronic microscope. We found that, at the early stage of infection, B. bassiana 12108 began to grow from joint of mouthparts, antennae, legs and joint base of chest foot. On the 10th day after inoculation, the hazelnut weevil was covered by dense hyphae, and its chest cavity was filled with dense hyphae as well. Simultaneously, a large amount of spores were formed on the body surface.Taken together, our results indicate that B. bassiana 12108 is highly virulence against hazelnut weevil, and provides scientific evidence for further developing of biological pesticide controlling hazelnut weevil.

       

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