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    郑丹, 李晓敏, 何静, 雷建都. 响应曲面法优化白木香叶总黄酮提取工艺及其纯化[J]. 北京林业大学学报, 2017, 39(8): 104-110. DOI: 10.13332/j.1000-1522.20160343
    引用本文: 郑丹, 李晓敏, 何静, 雷建都. 响应曲面法优化白木香叶总黄酮提取工艺及其纯化[J]. 北京林业大学学报, 2017, 39(8): 104-110. DOI: 10.13332/j.1000-1522.20160343
    ZHENG Dan, LI Xiao-min, HE Jing, LEI Jian-du. Optimization of extraction process for total flavonoids from leaves of Aquilaria sinensis by response surface method and purification technology[J]. Journal of Beijing Forestry University, 2017, 39(8): 104-110. DOI: 10.13332/j.1000-1522.20160343
    Citation: ZHENG Dan, LI Xiao-min, HE Jing, LEI Jian-du. Optimization of extraction process for total flavonoids from leaves of Aquilaria sinensis by response surface method and purification technology[J]. Journal of Beijing Forestry University, 2017, 39(8): 104-110. DOI: 10.13332/j.1000-1522.20160343

    响应曲面法优化白木香叶总黄酮提取工艺及其纯化

    Optimization of extraction process for total flavonoids from leaves of Aquilaria sinensis by response surface method and purification technology

    • 摘要: 以白木香叶为原料,利用乙醇水溶液浸提法,用芦丁为对照品,硝酸铝作显色剂,测定提取液在510 nm波长处的吸光度,来检测白木香叶总黄酮的提取率。单因素实验考察了乙醇体积分数、提取温度、液料比和提取时间4个因素对白木香叶总黄酮提取得率的影响,在此基础上,通过响应曲面法优化得到白木香叶总黄酮提取的最佳工艺条件。利用大孔吸附树脂对白木香叶总黄酮进行分离纯化,以饱和吸附量、吸附率和解吸率作为评价指标,比较了D101、AB-8和S-8 3种极性不同的大孔吸附树脂对白木香叶总黄酮的分离纯化效果,得到了分离纯化效果最佳的树脂,并研究了其吸附时间与吸附率的关系。结果表明:1)白木香叶总黄酮最佳提取条件为80%的乙醇、提取温度65 ℃、液料比20 mL/g、提取时间3 h,此条件下,白木香叶总黄酮得率为4.83%;2)非极性或弱极性的大孔吸附树脂更适合白木香叶总黄酮的分离纯化;3)D101大孔吸附树脂对白木香叶总黄酮具有较好的纯化效果,总黄酮纯度提高到纯化前的2.8倍。

       

      Abstract: The leaves of Aquilaria sinensis were extracted by ethanol solution, with rutin as reference substance and nitrate as chromogenic agent, the absorbance of the extract in the 510 nm wave was determined. In the single factor experiment, the effects of ethanol concentration, extraction temperature, solid-liquid ratio and extraction time on the extraction rate of total flavonoids were studied. On the basis of single factor experiment, the optimum conditions were studied by the response surface methodology. The total flavonoids in leaves of Aquilaria sinensis were separated and purified by macroporous adsorption resin. By comparing the saturated adsorption capacity, adsorption rate and desorption rate of three kinds of resins D101, AB-8, and S-8, the resin with the best separation and purification effect was obtained, and the relationship between the adsorption time and the adsorption rate was studied. The results showed that: 1) the optimal extraction conditions were 80% ethanol, 65 ℃, liquid to solid ratio of 20:1 (mL/g) and 3 hours as extraction time. Under this condition, the yield of total flavonoids was 4.83%. 2) Non-polar and low- polar macroporous adsorption resin were more suitable for the separation and purification of total flavonoids from the leaves of Aquilaria sinensis. 3) D101 macroporous adsorptive resin had best adsorption ability. The purity of flavones increased to 2.8 times.

       

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