ObjectiveThe fingerprint of the relative Quercus species was constructed and the genetic structure was analyzed, it provides an important tool for the identification and classification in relative Quercus species in order to classify and identify the relative Quercus species.
Method23 germplasms of relative Quercus were used as the materials in this study, SSR primers were selected by 4 accessions of genetically distant germplasms, 9 pairs of primers with clear amplification bands, high polymorphism and stable repeatability were selected and used for PCR amplification of the 23 germplasms, PCR products labeled fluorescent were detected using capillary electrophoresis technology, a strategy of combining primer pair with molecular weight for fingerprint construction was developed and applied to the 23 germplasms, the software of NTsys and STRUCTURE were used in clustering analysis and population genetic structure analysis.
ResultThe 78 fragments were amplified by 9 pairs of SSR primers, the number of alleles at each locus was between 4 and 14, with an average of 8.67 alleles for each pair of primers, polymorphism information content values for the primer pairs ranged from 0.58 to 0.82, with an average of 0.73. Clustering analysis showed that Quercus liaotungensis and Quercus monglica were respectively divided into two groups, Quercus coccinea and Quercus rubra were clustered into a group, population genetic structure analysis showed that 23 germplasms for the three groups, genetic structure analysis and clustering analysis results were consistent.
ConclusionThe 23 germplasms are divided into Quercus liaotungensis group, Quercus monglica group, Quercus coccinea-Quercus rubra mixed group, Quercus liaotungensis and Quercus monglica are two separate taxonomies, the results provide a theoretical basis for Quercus classification, variety identification and protection of intellectual property centers.