ObjectiveAnalysis of genetic structure of Pinus tabuliformis provenance can provide a theoretical basis for the exploration of the origin and trace to the source of Pinus tabuliformis artificial forest.
MethodIn this paper, SSR molecular markers were used to analyze the genetic structure and geographic system of the five Pinus tabuliformis populations in Shanxi Province of northern China. The test methods were analyzed with eight pairs of polymorphic primers and four differentially significant primers for chi-square detection.
ResultGenetic variation in Guandi Mountain was the most abundant, and that in Zhongtiao Mountain was the lowest. Eight pairs of primer analysis showed that the differentiation of five mountains was smaller(FST=0.032 8)and four pairs of primers analysis showed that there was a moderate degree of differentiation in five mountain populations(FST=0.051 9). There was a significant correlation between the genetic distance and geographical distance of the five mountain populations; the genetic diversity parameters had a negative correlation trend with temperature in January. The ratio of annual mean precipitation and temperature in January had a positive correlation trend with genetic diversity parameters.
ConclusionThe study reveals that there are relatively small genetic differentiation and rich genetic diversity among the geographical populations of Pinus tabuliformis in the main mountains of Shanxi Province. SSR markers can effectively display the genetic structure characteristics of various populations, and the limited difference marked markers can be more effectively applied to the population genetic analysis of Pinus tabuliformis. The relative level of water and heat of habitat meteorological factors has a dominant influence on the genetic diversity of population, and a relatively high ratio of water and heat is conducive to maintaining a higher genetic diversity of population.