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    符群, 吴桐, 廖声玲, 王梦丽. 刺玫果醇提物制备及其主要活性成分与抗氧化相关性[J]. 北京林业大学学报, 2019, 41(8): 131-137. DOI: 10.13332/j.1000-1522.20180227
    引用本文: 符群, 吴桐, 廖声玲, 王梦丽. 刺玫果醇提物制备及其主要活性成分与抗氧化相关性[J]. 北京林业大学学报, 2019, 41(8): 131-137. DOI: 10.13332/j.1000-1522.20180227
    Fu Qun, Wu Tong, Liao Shengling, Wang Mengli. Preparation of alcohol extract of Rosa davurica and the correlations between its main active components and antioxidant[J]. Journal of Beijing Forestry University, 2019, 41(8): 131-137. DOI: 10.13332/j.1000-1522.20180227
    Citation: Fu Qun, Wu Tong, Liao Shengling, Wang Mengli. Preparation of alcohol extract of Rosa davurica and the correlations between its main active components and antioxidant[J]. Journal of Beijing Forestry University, 2019, 41(8): 131-137. DOI: 10.13332/j.1000-1522.20180227

    刺玫果醇提物制备及其主要活性成分与抗氧化相关性

    Preparation of alcohol extract of Rosa davurica and the correlations between its main active components and antioxidant

    • 摘要:
      目的通过对北方林区刺玫果醇提物的制备及其主要活性成分进行研究,为刺玫果的进一步合理利用提供参考。
      方法采用高剪切乳化技术辅助乙醇提取刺玫果活性成分,在单因素试验基础上,以醇提物得率为指标,采用响应面法对主要工艺参数进行优化。对刺玫果醇提取物中总多酚、总黄酮、槲皮素和绿原酸进行提取,与DPPH·、OH·和·ABTS+的清除能力利用Pearson法做相关性研究。
      结果回归模型较好的反映了刺玫果黄酮醇提物与料液比、剪切转速、剪切时间的关系;确定最佳提取工艺为:料液比为1:25 g/mL,剪切转速为18 000 r/min,剪切时间为3 min,得率为(14.94 ± 0.47)%,回归模型的失拟值不显著,说明该回归模型模拟较好。绿原酸对DPPH·清除能力最强,在0.150 μg/mL时清除率达到80.84%;总黄酮对OH·清除能力最强,在0.484 μg/mL时清除率为82.32%;槲皮素对·ABTS+清除能力最强,在4.7 μg/mL时清除率达到91.32%。总黄酮与DPPH·、·OH、·ABTS+这3种自由基清除率相关系数最大,并与这3者清除能力的相关系数分别为 0.886、0.976、0.989(P < 0.01)。
      结论采用高剪切乳化技术辅助乙醇提取的刺玫果醇提物得率比普通超声辅助醇提法效果更好,醇提物得率增加了(2.11 ± 0.51)%,且不破坏刺玫果主要成分活性,刺玫果中总黄酮含量与抗氧化能力相关性最强。

       

      Abstract:
      ObjectiveIn this paper, the preparation of alcohol extract of Rosa davurica of the northern forest area and its main active ingredients were studied to provide reference for further rational utilization of Rosa davurica.
      MethodHigh-shear emulsification technology was used to assist ethanol extraction of active constituents of Rosa davurica. Based on the single factor experiment, the main process parameters were optimized by the response surface method based on the yield of alcohol extract. The total polyphenols, total flavonoids, quercetin, and chlorogenic acid contents in the extracts of Rosa davurica were determined, and the scavenging capacities of DPPH·, OH·, and ·ABTS+ were studied using the Pearson method.
      ResultThe regression model better reflected the relationship between the extract of flavonoids and the ratio of material to liquid, shearing speed and shear time. The optimal extraction process was as follows: the ratio of material to liquid was 1:25 g/mL, shearing speed for 18 000 r/min, the shear time was 3 min, and the yield was (14.94 ± 0.47)%. The regression model was not significant, indicating that the regression model was better. Chlorogenic acid had the highest DPPH· scavenging capacity, and the clearance rate reached 80.84% at 0.150 μg/mL; the total flavonoids had the strongest OH· scavenging capacity, and the clearance rate was 82.32% at 0.484 μg/mL; quercetin had the strongest ·ABTS+ scavenging ability. The clearance rate reached 91.32% at 4.7 μg/mL. The correlation coefficients between total flavonoids and DPPH·, OH·, ·ABTS+ free radical scavenging capacity were the highest, and the correlation coefficients between total flavonoids and DPPH·, OH·, ·ABTS+ free radical scavenging capacity were 0.886, 0.976 and 0.989, respectively (P < 0.01), indicating that the content of total flavonoids in Rosa davurica had the strongest correlation with antioxidant capacity.
      ConclusionThe high-shear emulsification technology assisted ethanol extraction of ethanol extracts was better than ordinary ultrasonic-assisted alcohol extraction, and the alcohol extract increased by (2.11 ± 0.51)%, and the activity of main constituents of Rosa davurica was not destroyed. The total flavonoid content in Rosa davurica has the strongest correlation with antioxidant capacity.

       

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