ObjectiveThe objective of this study is to analyze the genetic similarity, genetic relationship and geographical distribution characteristics of some wild Lilium species native to China. And this study provides useful information for molecularly assisted breeding of Lilium spp.
Method11 core SSR primers were used to scan 69 accessions of 25 wild species and 2 varieties in Lilium spp. by capillary electrophoresis platform and 69 samples were evaluated via the software Powermarker ver.3.25. The genetic similarity matrix and phylogenetic tree were constructed by NTsys 2.11 for cluster analysis. Principal coordinate analysis was performed using NTSYS-2.11.
ResultBased on the analysis of genetic parameters of SSR markers, the results showed that the SSR loci were polymorphism. The genetic similarity coefficient of 69 samples varied from 0.289 7 to 0.981 3. Among the different species, the genetic similarity coefficient of L. sulphureum and L. sargentiae was the largest, with an average of 0.953 3; while the genetic similarity coefficient of L. lancifolium and L. amoenum, L. lancifolium and L. taliense was the smallest, with an average of 0.289 7. Cluster analysis showed that 69 materials were divided into 5 groups, Group I consisted of the Cardiocrinum giganteum. The second group mainly consisted of the sect. Sinomartagon and the sect. Leucolirion, as well as the sect. Martagon and the sect. Lophophorum. Group III, IV and V are mainly composed of the sect. Sinmartagon, and including the Nomocharis forrestii. The main coordinate analysis showed that the Lilium species in Sichuan, Chongqing and Yunnan regions mostly gathered together. The Lilium species in Shanxi, Hubei, Hunan and Henan provinces mostly gathered together. The Lilium species in Liaoning Province and Inner Mongolia Autonomous Region mostly gathered together.
ConclusionThis study shows that subsect. Leucolirion a is obviously far away from subsect. Leucolirion b, but L. longiflorum and L. formosanum from subsect. Leucolirion a are closely related with sect. Sinomartagon. Besides, the Cardiocrinum giganteum is obviously far away from the Lilium spp. The relationship between Nomocharis forrestii and Liliun spp. is close, supporting the view that the Nomocharis is classified into the Lilium. At the same time, this study proves that with the increase of regional difference, the genetic similarity coefficient between Lilium in different provenances was decreased, which leading to their evolutionary differences. Different species of Llilium in the same provenance have the same spatial response to the environment, and they may have a trend towards convergence evolution.