金叶榆ISSR 识别卡构建及8 份榆属植物材料的亲缘关系分析
Construction of ISSR identification card of Ulmus pumila Jinye' and analysis of genetic diversity of 8 Ulmus materials.
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摘要: 采用ISSR 分子标记技术对8 份(品种、类型)榆属植物材料进行遗传多样性分析,从78 个引物中筛选出12 个具有扩增多态性的引物用于ISSR-PCR 扩增,共扩增出74 条带,其中多态性条带63 条,占总扩增条带的85%。 用NTSYSpc2-10e 软件对ISSR 扩增结果进行分析,结果表明,供试材料具有较丰富的遗传多样性。利用扩增条带 存在赋值1,否则赋值0 的数据分析方法建立金叶榆ISSR 识别卡;利用UPGMA 法进行聚类分析,将8 份(品种、类 型)植物材料按亲缘关系远近分为4 类。Abstract: Inter-simple sequence repeat (ISSR) marker was used to analyze genetic diversity of 8 improved breeds of Ulmus spp. Twelve primers screened out from 78 primers were used for ISSR-PCR. A total of 74 bands were generated, of which 63 bands appeared to be polymorphic, occupied 85% of total. The amplification results of ISSR were analyzed by NTSYSpc 2-10e software, and indicated that there actually existed high genetic diversity at molecular level among the 8 Ulmus materials. The method of amplified band assignment in 1,otherwise 0 was used to establish a ISSR identification card. These 8 improved breeds of Ulmus materials were divided into four groups with ISSR marker by UPGMA method.