非洲桃花心木不同地理群体遗传多样性分析

Analysis of genetic diversity from different geographical populations in Khaya senegalensis

摘要: 应用SRAP 分子标记技术,对来自非洲的5 个不同地理群体非洲桃花心木的遗传多样性和遗传分化进行了分析。结果表明:筛选出28 对SRAP 引物组合用于群体遗传分析,共扩增出条带77 条,其中多态性条带59 条,多态性水平为76.62%;5 个群体遗传距离在0.036 1 ~ 0.131 7 之间,总的Nei爷s 基因多样性指数为0.209 3,总的 Shannon爷s 信息指数分别为0.330 0,遗传变异主要来自群体内个体间(70.04%),群体基因流(Nm)为1.185 8。

Abstract: The genetic diversity and differentiations among five geographic populations of Khaya senegalensis were analyzed using sequence-related amplified polymorphism ( SRAP ) markers in this study. 28 primers were chosen from 200 for SRAP polymorphic analysis. The results showed that 59 bands were polymorphic, making up 76.62% of the total 77 amplified bands. The genetic distance among different K. senegalensis populations was 0.036 1 - 0.131 7. The general Nei's genetic diversity index was 0.209 3. The general Shannon's information index was 0.330 0. Genetic variation was mainly from individuals within groups (70.04%), and the gene flow between populations (Nm) was 1.185 8.