Optimization of extraction technology of ginsenosides from ginseng leaves by response surface method
-
摘要: 为优化人参叶总皂苷提取工艺,采用单因素试验,考察温度、时间、液料比对人参叶中总皂苷得率的影响。再采用3因素3水平的响应面分析法确定人参叶总皂苷提取的优化工艺,同时建立人参叶总皂苷提取的二次项数学模型,并验证其可靠性。结果表明:热水浸提法提取人参叶总皂苷的最佳条件为温度100 ℃、时间4.2 h、液料比16。在最佳条件下,人参叶总皂苷得率为人参叶质量的14.23％。Abstract: In order to optimize the extraction process of total ginsenosides from ginseng leaves, firstly we utilized one-factor experiment to investigate the influences of the factors (temperature, treatment time, and liquid -material ratio) on the extraction yield of total ginsenosides. Then we determined the optimal extraction process by the response surface methodology with 3 factors and 3 levels, and established the quadratic mathematical model for the ginsenosides extraction, and finally verified its reliability. The optimal extraction conditions for the hot water extraction of total ginsenosides were as follows: temperature 100 ℃, treatment time 4.2 h, liquid-material ratio 16. Under this condition, the yield of total ginsenosides from ginseng leaves was 14.23% of ginseng leaves’ mass.
-
Keywords:
- ginseng leaves /
- ginsenosides /
- extraction /
- response surface methodology
-
-
[1] 张春枝,安利佳,金凤燮.人参皂苷生理活性的研究进展[J].食品与发酵工业,2002,28(4):70-73. [1] ZHANG C Z, AN L J, JIN F X. Progress on the research of physiological function of ginsenosides[J]. Food and Fermentation Industries, 2002, 28(4): 70-73.
[2] 赵文莉,张馨,王伟,等.人参茎叶总皂甙抗疲劳作用的体内实验研究[J].卫生研究,2009,38(2):184-187. [2] ZHAO W L, ZHANG X, WANG W, et al. Experimental study for the anti-fatigue effect of ginseng general ginsenosides P. E. in vivo[J]. Journal of Hygiene Research, 2009, 38(2): 184-187.
[3] 张晓文,宋清,徐志伟.人参茎叶总皂苷对心理应激荷瘤小鼠肿瘤生长及化疗效果的影响[J].现代食品科技,2010,26(4):348-350. [3] ZHANG X W, SONG Q, XU Z W. Influences of ginseng stem and leave saponin on tumor growth of tumor-bearing mouse on psychological stress status and the chemo-therapy effect[J]. Modern Food Science and Technology, 2010, 26(4): 348-350.
[4] LÜ Y, ZHU P D, FENG X M, et al. The effect of total saponin of panax ginseng and panaxquinquefoliuml saponin on hematopoiesis in anemic mice[J]. Journal of Chengdu University of Traditional Chinese, 2005, 28(2): 33-42.
[4] 吕艳,祝彼得,冯雪梅,等.人参总皂甙和西洋参茎叶总皂甙对贫血小鼠造血影响的比较[J].成都中医药大学学报, 2005,28(2):33-42. [5] 张晶,陈全成,弓晓杰,等.不同提取方法对人参皂苷提取率的影响[J].吉林农业大学学报,2003,25(1):71-73. [5] ZHANG J, CHEN Q C, GONG X J, et al. Effect of different methods on extracting ratios of ginsenosides[J]. Journal of Jilin Agricultural University, 2003, 25(1): 71-73.
[6] 赵一晖,张培军,战英,等.紫外分光光度法测定人参叶茶中人参总皂苷的方法学研究[J].中国卫生工程学,2012,11(1):63-64. [6] ZHAO Y H, ZHANG P J, ZHAN Y, et al. Determination of ginsenosides in ginseng leaf tea by ultraviolet spectrophotometry[J]. Chinese Journal of Public Health Engineering, 2012, 11(1): 63-64.
[7] 于杰,白志川,付超美.人参茎叶总皂甙提取精制工艺研究[J].西南农业大学学报,2004,26(4):479-482. [7] YU J, BAI Z C, FU C M. Research of the technology of extraction and puprification of total saponin from the stem and leaf of Panax Ginseng[J]. Journal of Southwest Agricultural University, 2004, 26(4): 479-482.
[8] 白雪莲,岳田利,章华伟,等.响应曲面法优化微波辅助提取苹果渣多酚工艺研究[J].中国食品学报,2010,10(4):169-177. [8] BAI X L, YUE T L, ZHANG H W, et al. Optimization of microwave-assisted extraction technology of polyphenols from apple pomace by response surface method [J]. Journal of Chinese Institute of Food Science and Technology, 2010, 10(4): 169-177.
[9] YUE X Q, BAO H Y, YU N, et al. Study on response surface extraction process of yolk protein from egg yolk powder[J]. Food Research and Development, 2011, 32(4): 48-52.
[9] 岳喜庆,鲍宏宇,于娜,等.响应面法优化卵黄蛋白质提取工艺[J].食品研究与开发,2011, 32(4):48-52. [10] 王尉,徐春明,曹学丽,等.响应面法优化微波辅助提取桑叶多糖的工艺研究[J].北京工商大学学报,2009,27(4):6-9. [10] WANG W, XU C M, CAO X L, et al. Optimization of microwave-assisted extraction of polysaccharide from Morus alba L. by response surface methodology[J]. Journal of Beijing Technology and Business University, 2009, 27(4): 6-9.
[11] 张泽志,高霞,林钰,等.用响应面法优化微波提取花生壳总黄酮工艺[J].河南工业大学学报,2010,31(4):32-36. [11] ZHANG Z Z, GAO X, LIN Y, et al. Optimization of microwave-assisted extraction of total flavones from peanuthull by response surface analysis[J]. Journal of Henan University of Technology, 2010, 31(4): 32-36.
[12] FRANCIS F, SABU A, NAMPOOTHIRI K M, et al. Use of response surface methodology for optimizing process parameters for the production of α-amylase by Aspergillus oryzae[J].Biochemical Engineering Journal, 2003, 15(2): 107-115.
[13] ELIBOL M. Optimization of medium composition for actinorhodin production by Streptomyces coelicolor A3(2) with response surface methodology[J]. Process Biochemistry, 2004, 39(9): 1057-1062.
[14] KALIL S J, MAUGERI F, RODRIGUES M I. Response surface analysis and simulation as a tool for bioprocess design and optimization[J]. Process Biochemistry, 2000, 35(6): 539-550.
-
期刊类型引用(11)
1. 邢艺缤，王馨悦，王慕尧，齐欣，崔承弼. 人参不定根总皂苷的提取工艺优化及其抗氧化与抗疲劳作用. 食品工业科技. 2024(06): 193-201 . 
百度学术
2. 段玺，巨红叶，焦莹，唐志书，宋逍. 基于响应面法优化的倒卵叶五加多糖的超声波提取工艺研究. 河南中医. 2019(01): 137-141 . 百度学术
3. 吴冕，孙墨珑. 核桃楸外果皮总蒽醌的提取及抗氧化性研究. 森林工程. 2019(03): 48-53 . 百度学术
4. 黄文静，孙晓春，姚绍嫦，潘春柳，龚明霞，何海旺. 基于PB-CCD设计的黄芪茎叶总皂苷提取工艺优化及其抗氧化活性研究. 基因组学与应用生物学. 2019(08): 3690-3697 . 百度学术
5. 薛思慧，张枫源，向福，张晓红，项俊. 刺楸叶总皂苷的酶法提取及其抗氧化活性评价. 中国酿造. 2018(02): 142-147 . 百度学术
6. 金银萍，逄世峰，焉石，王博，郭靖，王英平. Box-Behnken法优化不同类型人参皂苷提取工艺. 吉林农业大学学报. 2017(02): 162-168+175 . 百度学术
7. 刘成禹，侯万超，李森林，李赛男. 人参叶和车前草配伍的体内抗氧化活性评价研究. 时珍国医国药. 2017(12): 2817-2820 . 百度学术
8. 张语迟，李赛男，刘春明，唐英，李森林. 人参叶提取物的提取工艺及抗氧化活性评价研究. 中华中医药学刊. 2017(02): 326-329 . 百度学术
9. 赵玉红，林洋，张智，王振宇. 碱溶酸沉法提取黑木耳蛋白质研究. 食品研究与开发. 2016(16): 32-36 . 百度学术
10. 邵青，贺晓龙，张昊，王延峰. 响应面法优化山丹鳞茎皂甙超声提取工艺的研究. 延安大学学报(自然科学版). 2016(01): 58-62 . 百度学术
11. 陈斯玮，王芳，章卓，张丹. Box-Behnken响应面法优化川佛手挥发油的超临界CO_2萃取工艺. 中国药房. 2016(28): 3973-3975 . 百度学术
其他类型引用(12)
计量
- 文章访问数: 1472
- HTML全文浏览量: 109
- PDF下载量: 26
- 被引次数: 23
下载:
