Abstract: In order to optimize the extraction process of total ginsenosides from ginseng leaves, firstly we utilized one-factor experiment to investigate the influences of the factors (temperature, treatment time, and liquid -material ratio) on the extraction yield of total ginsenosides. Then we determined the optimal extraction process by the response surface methodology with 3 factors and 3 levels, and established the quadratic mathematical model for the ginsenosides extraction, and finally verified its reliability. The optimal extraction conditions for the hot water extraction of total ginsenosides were as follows: temperature 100 ℃, treatment time 4.2 h, liquid-material ratio 16. Under this condition, the yield of total ginsenosides from ginseng leaves was 14.23% of ginseng leaves’ mass.