Expression analysis of carbonic anhydrase gene family in Populus simonii × P nigra
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摘要: 本文克隆了小黑杨CA家族的6个基因成员,分别命名为PsnCA1、PsnCA2、PsnCA3、PsnCA4、PsnCA5和PsnCA6,其中PsnCA4和PsnCA6属于α碳酸酐酶家族,PsnCA1、PsnCA3和PsnCA5属于β碳酸酐酶家族,而PsnCA2属于LbeatH超基因家族。系统进化分析表明,亲缘关系较近的是PsnCA2、PsnCA4和PsnCA6,而PsnCA1、PsnCA3和PsnCA5聚为一大类。通过实时荧光RT-PCR技术研究了小黑杨根、茎、叶中PsnCA基因的表达模式,结果表明:除PsnCA6外,其余5个基因在叶部的表达量显著高于根和茎。在叶部表达量最高的为PsnCA1,在茎部和根部表达最高的为PsnCA3。PsnCA在各部位的表达均受暗处理的影响,其中各基因在根部的表达变化最大,其次为茎和叶。Abstract: In this study, we isolated six members of the CA family of genes, namely PsnCA1,PsnCA2,PsnCA3,PsnCA4,PsnCA5 and PsnCA6 respectively in Populus simonii×P. nigra.Of which PsnCA4 and PsnCA6 belonged to the family of α carbonic anhydrase,and PsnCA1,PsnCA3 and PsnCA5 belonged to the family of β carbonic anhydrase,and PsnCA2 belonged to LbeatH super gene family. The results of phylogenetic classification showed that PsnCA1, PsnCA3 and PsnCA5 were clustered into one category, while PsnCA2, PsnCA4 and PsnCA6 belonged to another category. Real-time PCR was used to investigate the PsnCA gene expression pattern in roots, stems and leaves, and the results shows: except PsnCA6, the remaining five gene expression levels in the leaves were significantly higher than the roots and stems. The highest expression in the leaves was PsnCA1, and the highest expression in the stems and roots was PsnCA3. PsnCA expression governed by the dark treatment in various tissues, the largest gene expression changed in roots,followed by the stems and leaves.
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[1] SMITHS K S,JAKUBZICK C,WHITTAM T S,et al.Carbonic anhydrase is an ancient enzyme widespread in prokaryotes[J]. PNAS,1999,96:15184-15189.
[1] JIANG C Y,MA X L,SHEN X Y,et al.Plant progress in research on plant carbonic anhydrase[J]. Plant Physiology Journal,2013,49(6):545-550.
[2] TERASHIMA I,ONO K.Effects of HgCl2 on CO2 dependence of leaf photosynthesis: evidence indicating involvement of aquaporinsin CO2 diffusion across the plasma membrane[J].Plant and Cell Physiology,2002,43(1):70-78.
[2] YU L J,WU Y,LI W,et al.Study on stability of extracellular carbonic anhydrase from soil bacteria in karst areas of Southwest China[J].Life Science Research,2004,8(4):365-370.
[3] YANG X,LIU Z H,CAO J H ,et al.Study on the relationship between photosynthesis of maize and the zinc content and carbonic anhydrase activity in karst and non-karst areas[J].Carsologica Sinica,2008,27(2):103-107.
[3] JEBANATHIRAJAH J A,COLEMAN J R.Association of carbonic anhydrase with a Calvin cycle enzyme complex in Nicotianatabacum[J].Plant,1999,204:177-182.
[4] MORONEY J V,BARTLETT S G,SAMUELSSON G.Carbonic anhydrases in plant and algae[J].Plant Cell and Environment,2001,24:141-153.
[4] ZHANG K X,ZHAO G Y,WEI Z G,et al.Comparative analysis of two cDNA libraries from Populus simonii×P. nigra tension wood[J].Journal of Beijing Forestry University,2011,33(3):8-13.
[5] WANG Y C,YANG C P,JIANG J.Method of extracting RNA quickly from syringa oblata and Acer negundo[J].Journal of Northeast Forestry University,2001,29(6):90-91.
[5] GULIEV N M,BAIRAMOV S M,ALIEV D A.Functional organization of carbonic anhydrase in higher plants[J].Plant Physiol,1992,39:537-544.
[6] WANG Q,FRISTED R,YU X,et al.The γ-carbonic anhydrase subcomplex of mitochondrial complex I is essential for development and important for photomorphogenesis of Arabidopsis[J]. Plant Physiol,2012,160:1373-1383.
[6] SUN G C, LIN Z F, LIN G Z.The Carboxylation rate of rubisco and activity of carbonic anhydrase in plants from a subtropical forest grown at different light intensity[J].Journal of Wuhan Botanical Research,2001,19(4):304-310.
[7] PANDEY N,SHARMA C P.Zinc deficiency effect on photosynthesis and transpiration in safflower and its reversal on making up the deficiency[J].Indian J Exp Biol,1989,27:376-377.
[8] SHIRAIWA Y,KIUYAMA M.Role of carbonic anhydrase and identification of the active species of inorganic carbon utilized for photosynthesis in Characorallina[J].Plant Cell Physiol,1989,30(4):581-587.
[9] JACOBSON B S,FONG F,HEATH R L.Carbonic anhydrase of spinach[J].Plant Physiol,1975,55:468-474.
[10] 蒋春云,马秀灵,沈晓艳,等.植物碳酸酐酶的研究进展[J].植物生理学报,2013,49(6):545-550. [11] TSUZUKI M,MIYACHI S,EDWARDS G E.Localization of carbonic anhydrase in Mesophy II cells of terrestrial C3 plants in relation to CO2 assimilation[J].Plant Cell Physiol,1985,26:881-891.
[12] 余龙江,吴云,李为,等.西南岩溶区土壤细菌胞外碳酸酐酶的稳定性研究[J].生命科学研究,2004,8(4):365-370. [13] 杨霄,刘再华,曹建华,等.岩溶区和非岩溶区玉米光合作用与锌含量和碳酸酐酶关系的对比研究[J].中国岩溶,2008,27(2):103-107. [14] 张凯旋,赵桂媛,魏志刚,等.小黑杨应拉木上下侧cDNA文库的比较分析[J].北京林业大学学报,2011,33(3):8-13. [15] 王玉成,杨传平,姜静.紫丁香、糖槭总RNA的快速提取方法[J].东北林业大学学报,2001,29(6):90-91. [16] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression da-tausing real-time quantitative PCR and the 2( -DeltaDeltaC(T)) Method[J]. Methods,2001,25:402-408.
[17] 孙谷畴,林植芳,林桂珠.不同光强下生长的几种亚热带森林树木Rubisco羧化速率和碳酸酐酶的活性[J].武汉植物学研究,2001,19(4):304-310. [18] MERCAD J M,CARMONA R,NIELL F X.Affinity for inorganic carbon of Gracilaria fanuistiptata cultured at low and high irradiance[J].Planta,2000,210(5):758-764.
[19] JI B H,LI X,JIAO D M.Some physiological function and adaptive regulation of carbonic anhydrase under photoinhibition condition in rice[J].Chinese J Rice Sci,1997,11(4):238-240.
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