Ploidy level and contrast analysis of the traits for superior trees of Populus tomentosa Carr. in gene pool
-
摘要: 以山东冠县毛白杨基因库内的优树为对象,采用流式细胞仪对基因库内469个毛白杨无性系的染色体倍性进行检测,并对检测出来的毛白杨天然三倍体和邻近的同种源二倍体对照的生长、形质和叶片气孔大小等性状变异进行研究。结果表明:在基因库内共检测出28个毛白杨天然三倍体无性系,占基因库无性系总数的6.0%,包括24个雌性三倍体无性系、4个雄性三倍体无性系,其中北京2个、河北12个、山东1个、山西2个、陕西11个,分别占基因库无性系总数的0.4%、2.6%、0.2%、0.4%和2.3%;毛白杨天然三倍体在生长、形质和叶片气孔特性等性状上存在共性,与二倍体相比,具有生长量大、侧枝粗、气孔大而稀疏等特点;天然三倍体无性系和二倍体无性系在胸径、材积、侧枝粗、气孔长度、气孔宽度、气孔密度等均存在极显著差异,树高存在显著差异,且不同天然三倍体无性系间的叶片气孔长度、气孔宽度、气孔密度间的差异也达到了极显著性水平;此外,毛白杨天然三倍体群体中也有部分个体表现出类似二倍体的特征,其中存在一些在生长等性状表现较差的无性系,表明天然三倍体无性系并非株株均优,毛白杨天然三倍体应用时同样有必要进行选择。Abstract: We used the flow cytometry to determine the ploidy level of 469 clones of Populus tomentosa in a gene pool located at Guan County of Shandong Province and analyzed the variations in growth, form quality and stomatal traits for identified natural triploid clones and their neighboring diploid clones of P. tomentosa from the same provenance. Twenty-eight natural triploid clones of P. tomentosa (24 female and 4 male clones) were determined, accounting for 6.0% of the total clones in the pool. Among those triploid clones, 2 were derived from Beijing, 12 from Hebei, 1 from Shandong, 2 from Shanxi and 11 from Shaanxi, which accounted for 0.4%, 2.6%, 0.2%, 0.4% and 2.3% of the total clones in the pool, respectively. There were similar characteristics such as growth, form quality and stomatal traits among natural triploid clones of P. tomentosa. Compared with diploid clones, triploid clones had characteristics of faster growth, thicker branch diameter, and bigger and sparser stomas. There were extremely significant differences between natural triploid and diploid control clones of P. tomentosa in diameter at breast height (DBH), volume of timber, branch diameter, stomatal length, stomatal width and stomatal density, and significant differences in height. Extremely significant differences in stomatal traits among all identified natural triploid clones were also observed. However, some of natural triploid clones of P. tomentosa had similar characteristics with diploid clones; some of them had worse growth traits. Our results indicated that not each of the natural triploid clones of P. tomentosa was superior. Therefore selection is still necessary while applying natural triploid clones of P. tomentosa.
-
Key words:
- Populus tomentosa /
- natural triploid /
- growth trait /
- stomatal traits /
- genetic variation
-
[1] NILSSON-EHLE H. Note regarding the gigas form of Populus tremula found in nature[J]. Hereditas, 1936, 21:372-382. [2] KANG X Y. Advances in researches on polyploid breeding of forest trees[J]. Journal of Beijing Forestry University,2003,25(4):70-74. [3] VAN DILLEWIJIN C. Cytology and breeding of Populus [J]. Ned Boschb Tijdschr, 1939, 12: 470-481. [4] KANG X Y. Some understandings on polyploid breeding of poplars[J]. Journal of Beijing Forestry University,2010,32(5):149-153. [5] GURREIRO M G. The silvicultural improvement of Populus[J]. Publ Serv Flor Aquic, Portugal, 1944, 11(1-2): 53-117. [6] XIAO Y,YANG Y D, XIA W, et al. Significance of polyploidy evolutionary in flowering plants[J]. Guangdong Agricultural Sciences,2013,40(16):127-130. [7] ZHU Z T, KANG X Y,ZHANG Z Y. Studies on selection of natural triploids of Populus tomentosa[J]. Scientia Silvae Sinicae,1998,34(4):22-31. [8] VAN BUIJTENEN J P, JORANSON P N, EINSPAHR D W. Naturally occurring triploid quaking aspen Populus tremuloides in the United States[M]. Albuquerque: Proc Soc Amer For, 1957: 62-64. [9] KANG X Y. Chromosome count and shape of poplar[J]. Journal of Gansu Agricultural University,1996,31(1):25-29. [10] SEITZ F W. Results of selfing and crossing using pollen of the Dillingen androgynous grey poplar[J]. Abstr in Z Forstgenet, 1954, 3(6): 141. [11] KANG X Y, WANG J. Poplar polyploid induction technology research[M]. Beijing:Science Press,2010. [12] MANZOS A M. Fast-growing form of Populus balsamifera obtained by pollinating female flowers with fractionated pollen of the same species[J]. Doklady Akademii Nauk SSSR, 1960, 131(2): 433-435. [13] CHEN Z S. The establishment of binomial stock volume tables of poplar[J]. Forest Research, 1989,2(1):78-83. [14] EVERY A D, WIENS D. Triploidy in Utah aspen[J]. Madroño, 1971, 21(3): 138. [15] EINSPANHR D W. Colchicine treatment of newly formed embryos of quaking aspen[J]. Forest Science, 1965, 11(4): 456-459. [16] LIU M Z, PAN X L. Apply nail polish removal plant leaf stomatal slice preparation[J]. Bulletin of Biology,2006,40(10):44. [17] ZHU Z T, LIN H B, KANG X Y. Studies on allotriploid breeding of Populus tomentosa B301 clones[J]. Scientia Silvae Sinicae,1995,31(6):499-505. [18] 康向阳.林木多倍体育种研究进展[J].北京林业大学学报,2003,25(4):70-74. [19] CHEN C B, QI L W, ZHANG S G, et al. The karyptype analysis of triploid poplar[J]. Journal of Wuhan Botanical Research, 2004,22(6):565-567. [20] 康向阳.关于杨树多倍体育种的几点认识[J].北京林业大学学报,2010,32(5):149-153. [21] 肖勇,杨耀东,夏薇,等.多倍体在植物进化中的意义[J].广东农业科学,2013,40(16):127-130. [22] ZHANG S G, CHEN C B, HAN S Y, et al. Chromosome numbers of some Populus taxa from China[J]. Journal of Systematics and Evolution,2005,43(6):539-544. [23] WANG J.Techniques of polyploid induction in Populus spp. (Section Tacamahaca)[D].Beijing: Beijing Forestry University, 2009. [24] ZHU Z T, ZHANG Z Y.The status and advances of genetic improvement of Populus tomentosa Carr.[J]. Journal of Beijing Forestry University: English Edition, 1997, 6(1): 1-7. [25] SHI L, ZHANG P D, MAO Y K, et al. Polyploid induction of Populus hybrid through zygotic chromosome doubling with high-temperature treatment[J]. Acta Agriculturae Nucleatae Sinica,2012,26(8):1118-1123. [26] 朱之悌,康向阳,张志毅.毛白杨天然三倍体选种研究[J].林业科学,1998,34(4):22-31. [27] 康向阳.杨树染色体数目和形态观察[J].甘肃农业大学学报,1996,31(1):25-29. [28] KANG X Y. Cytogenetics and selection of triploids of Populus tomentosa[M].Beijing:China Environmental Sciences Press, 2002. [29] 康向阳,王君.杨树多倍体诱导技术研究[M].北京:科学出版社,2010. [30] 陈章水.杨树二元立木材积表的编制[J].林业科学研究,1989,2(1):78-83. [31] 刘明智,潘晓玲.指甲油涂抹撕取法制取植物叶气孔装片[J].生物学通报,2006,40(10):44. [32] JOHNSSON H. Development of triploid and diploid Populus tremula during the juvenile period[J]. Z Forstgenet, 1953, 2(4): 73-77. [33] EINSPHAHR D W. Production and utilization of triploid hybrid aspen [Populus tremuloides, Populus tremula][J]. Iowa State Journal of Research, 1984,58:401-409. [34] WEISGERBER H,RAU H M, GARTNER E J, et al. 25 years of forest tree breeding in Hesse[J]. Allgemeine Forstzeitschrift, 1980,26:665-712. [35] 朱之悌,林惠斌,康向阳.毛白杨异源三倍体B301等无性系选育的研究[J].林业科学,1995,31(6):499-505. [36] 陈成彬,齐力旺,张守攻,等.三倍体杨树核型分析[J].武汉植物学研究,2004,22(6):565-567. [37] 张守攻,陈成彬,韩素英,等.中国部分杨属植物的染色体数目[J].植物分类学报,2005,43(6):539-544. [38] 王君.青杨派树种多倍体诱导技术研究[D].北京:北京林业大学,2009. [39] 石乐,张平冬,毛彦科,等.高温处理诱导染色体加倍获得白杨杂种多倍体[J].核农学报,2012,26(8):1118-1123. [40] 康向阳.毛白杨细胞遗传与三倍体选育[M].北京:中国环境科学出版社,2002. -
点击查看大图
计量
- 文章访问数: 469
- HTML全文浏览量: 62
- PDF下载量: 12
- 被引次数: 0
下载:
