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    赵丽红, 周菲, 官民晓, 刘闯, 赵鑫, 刘雪梅. 白桦雌花发育前期和中期差异基因表达分析[J]. 北京林业大学学报, 2015, 37(4): 104-112. DOI: DOI:10.13332/j.1000-1522.20140301
    引用本文: 赵丽红, 周菲, 官民晓, 刘闯, 赵鑫, 刘雪梅. 白桦雌花发育前期和中期差异基因表达分析[J]. 北京林业大学学报, 2015, 37(4): 104-112. DOI: DOI:10.13332/j.1000-1522.20140301
    ZHAO Li-hong, ZHOU Fei, GUAN Min-xiao, LIU Chuang, ZHAO Xin, LIU Xue-mei. Analysis of differentially expressed transcripts in early and middle development of Betula platyphylla female inflorescence[J]. Journal of Beijing Forestry University, 2015, 37(4): 104-112. DOI: DOI:10.13332/j.1000-1522.20140301
    Citation: ZHAO Li-hong, ZHOU Fei, GUAN Min-xiao, LIU Chuang, ZHAO Xin, LIU Xue-mei. Analysis of differentially expressed transcripts in early and middle development of Betula platyphylla female inflorescence[J]. Journal of Beijing Forestry University, 2015, 37(4): 104-112. DOI: DOI:10.13332/j.1000-1522.20140301

    白桦雌花发育前期和中期差异基因表达分析

    Analysis of differentially expressed transcripts in early and middle development of Betula platyphylla female inflorescence

    • 摘要: 白桦为雌雄同株的单性花树种,在木本植物花发育研究中有着重要作用。本文旨在初步确定白桦雌花发育过程中的关键时期大孢子发生(前期)和配子体发育(中期)过程中基因表达及调控的差异,同时挖掘与雌花发育相关的基因。首先,运用细胞学方法确定试材雌花的发育时期。其次,采用抑制消减杂交(SSH)技术,分别以白桦前期和中期雌花序的cDNA作为tester和driver,建立正、反向抑制消减杂交cDNA文库:BHHQ和BHHZ。共获得1406个高质量的EST序列,其中787个与数据库中的已知核酸或蛋白序列有较高同源性, 47个可能与花发育相关。GO分析结果表明,这些基因34.96%参与生物合成,16.45%参与细胞组件,48.59%参与分子功能。另外,选择6个与花发育相关EST进行qRT-PCR分析,结果显示这些基因在不同组织中都有一定表达。其中bhhq.408,bhhq.435,bhhz.359,bhhz.215在生殖器官中的表达明显高于营养器官;bhhq.254,bhhq.190在绝大多数组织中均有一定表达,说明这些基因不仅参与生殖器官的发育,也在营养器官中发挥一定的作用。

       

      Abstract: Betula platyphylla is a monoecious tree species with unisexual flowers, which plays an important role in the study of floral development of woody plants. This study aims to initially identify gene expression and regulation during the critical periods of female flower development in B. platyphylla, megasporogenesis (prophase) and gametophyte development (metaphase), and to discover genes related to female floral development. Firstly, we ascertained the prophase and metaphase in female floral development by the cytological method. Secondly, the establishment of forward and reverse suppression subtractive hybridization (SSH) cDNA libraries BHHQ and BHHZ were carried out with the cDNA of prophase and metaphase female inflorescence as tester and driver respectively, using SSH technique. A total of 1406 high quality EST sequences were obtained, 787 of them having high homologies with the nucleic acid or protein sequences in databases and 47 genes possibly associating with flower development. The results of GO analysis showed that 34.96% of these genes are mainly involved in biosynthesis process, 16.45% in cellular component and 48.59% in molecular function. Six flower development-related genes were analyzed by qRT-PCR analysis and the results showed that each of these genes has some expression in different tissues. Among them, the expression level of bhhq.408,bhhq.435,bhhz.359, and bhhz.215 in reproductive organs was significantly higher than that in other organs, respectively; both bhhq.254 and bhhq.190 have some expression in most tissues, confirming that these genes are not only involved in the development of reproductive organs, but also play some roles in vegetative organs.

       

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