Abstract: In order to establish an effective regeneration system of Lespedeza formosa, the effects of 6-BA,2,4-D,NAA and seedling age on adventitious buds regeneration,proliferation and rooting of L.formosa were studied.The results showed that 6-BA and 2,4-D could evidently accelerate adventitious buds regeneration on cotyledonary node of L.formosa,the optimum regeneration medium was MS+2,4-D(1.0 mg/L)+6-BA 0.5 mg/L+NAA 0.5 mg/L.There were not obvious differences in different seedling ages,but the older cotyledonary node was more sensitive to 6-BA.6-BA affected obviously the differentiation of adventitious buds in segment proliferation,MS+6-BA 1.0 mg/L +NAA 0.1 mg/L was a optimum proliferation medium.It was not 6-BA but NAA that play an important role in rooting,and the optimum rooting medium was MS+6-BA 0.15 mg/L+NAA 0.5 mg/L.