转抗鞘翅目害虫基因银腺杨的获得及其抗虫性的初步研究

Production of Populus alba×Pglandulosa with a coleopterous insect resistant gene and analysis of insect resistance

摘要: 培育抗鞘翅目害虫的杨树新品种是防治光肩星天牛等高危害蛀干害虫的有效方法之一.该研究通过根癌农杆菌介导法将抗鞘翅目害虫基因(Cry3A)导入银腺杨基因组中,获得了再生植株.PCR及Southern点杂交的分析结果表明,外源基因已整合到杨树基因组中.室内抗虫生物学鉴定表明,与对照相比,转基因株系BGA-5对光肩星天牛具有一定的毒杀和显著的生长抑制作用,其杀虫率达30%,对幼虫的生长抑制率达78.46%;其他转基因株系对光肩星天牛幼虫无明显的毒杀和生长抑制作用.

Abstract: Production of new poplar varieties is one of the effective methods of preventing trees from damage by stem-borers,such as Anoplophora glabripennis. In this study,transgenic poplar plants containing the Cry3A gene were obtained via an Agrobacterium-mediated method.The transgenic nature of these plants was confirmed by PCR amplification and Southern dot hybridization.The toxicity of these plants to Anoplophora glabripennis larvae has been assessed on two years old selected plants under laboratory conditions.The results indicated that the transgenic line BGA-5 was toxic to A.glabripennis larvae with 30% mortality and could inhibit the growth of A.glabripennis larvae with a 78.46% growth inhibition rate,while others did not show any toxic effect and growth inhibition affecting the larvae.