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    胡晓丽, 周春江, 岳良松. 三倍体毛白杨无性系的AFLP分子标记鉴定[J]. 北京林业大学学报, 2006, 28(2): 9-14.
    引用本文: 胡晓丽, 周春江, 岳良松. 三倍体毛白杨无性系的AFLP分子标记鉴定[J]. 北京林业大学学报, 2006, 28(2): 9-14.
    shu
    HU Xiao-li, ZHOU Chun-jiang, YUE Liang-song. Molecular identification of Populus tomentosa triploid clones by AFLP[J]. Journal of Beijing Forestry University, 2006, 28(2): 9-14.
    Citation: HU Xiao-li, ZHOU Chun-jiang, YUE Liang-song. Molecular identification of Populus tomentosa triploid clones by AFLP[J]. Journal of Beijing Forestry University, 2006, 28(2): 9-14.
    shu

    三倍体毛白杨无性系的AFLP分子标记鉴定

    Molecular identification of Populus tomentosa triploid clones by AFLP

      摘要
    • 摘要: 利用AFLP标记技术采用MseⅠ+3/EcoRⅠ+2引物组合,对12个三倍体毛白杨和二倍体毛白杨无性系进行了分析鉴定.从38对引物组合中选出12对扩增条带较多的引物组合,多态性比例最高的是M-CAT/E-TC,多态性水平达到32.4%,最低的M-CTT/E-TT只有15.7%,证明供试材料之间的亲缘关系较近;UPGMA聚类分析结果与供试材料的系谱关系以及形态鉴别结果一致,表明凡亲本相同或形态差异小的无性系具有较高的相似系数;利用3个引物组合的8条多态性条带构建了12个供试材料的指纹图谱,可以作为三倍体毛白杨无性系鉴别以及品种保护的依据.

       

      Abstract: Twelve Populus tomentosa triploids and diploids were identified by AFLP molecular technology using a MseⅠ+3/EcoRⅠ+2 primer combination.Twelve primers with high amplified bands were selected from 38 primer combinations,including primer M-AT/E-TC with the highest ratio of polymorphic sites(32.4%) as well as primer M-TT/E-TT with the lowest ratio of polymorphic sites(15.7%).The genetic relationships among the 12 materials are relatively close.The dendrogram result of 12 clones constructed with a UPGMA method is consistent with the pedigree relationship and morphological identification,which indicates that high similarity coefficients exist in the clones with the same parents or with small morphological differences.A fingerprinting map of 12 clones has been constructed by 8 polymorphic bands selected from 3 primer combinations,which can identify the true Populus tomentosa triploids and serve as the basis for species protection.

       

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