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    月季未成熟胚再生植株的研究

    Plant regeneration from immature embryos of roses

    • 摘要: 为了建立月季遗传转化的再生体系,该试验以‘漂多斯’、‘蓝丝带’、‘索奴拉’3个月季品种的未成熟胚为外植体,获得了月季再生植株.结果表明,在MS+ZT 0.5 mg/L、MS+ZT 4.0 mg/L+NAA 1.0 mg/L、MS+ZT 0.5 mg/L+NAA0.5 mg/L培养基上,‘漂多斯’、‘蓝丝带’、‘索奴拉’能够诱导出再生芽,再生率分别为33.3%、33.3%、16.7%;再生芽在不含植物生长调节剂的MS基本培养基上伸长、展叶,在MS+IBA 0.5 mg/L的培养基上能得到生根的再生植株.随着月季未成熟胚贮藏时间的延长,不仅污染率增加,而且胚成熟后多发育成胚芽;贮藏时间不宜超过90 d.这种以未成熟胚为外植体获得再生植株的途径,可为遗传转化提供有效的受体系统.

       

      Abstract: In order to obtain rose regeneration system for genetic transformation,the immature embryos of Rosa ‘Peaudouce’,‘Blue Ribbon’ and ‘Sonora’ were taken as explants,and regenerated plants were obtained.Adventitious buds were induced in MS+ZT 0.5 mg/L for ‘Peaudouce’,MS+ZT 4.0 mg/L+NAA 1.0 mg/L for ‘Blue Ribbon’ and MS+ZT 0.5 mg/L+NAA 0.5 mg/L for ‘Sonora’,and the regeneration rates were 33.3%,33.3% and 16.7% respectively.The regenerated buds grew into shoots in the MS medium without plant growth regulators,and they rooted in MS+IBA 0.5 mg/L.The contamination rate increased and the matured embryos prefer to develop into plumules when the storing time prolonged;the longest time for storing was less than 90 days.The results indicate that the plantlet regeneration system from immature embryos can be a proper receptor system for genetic transformation in roses.

       

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