Abstract: In order to calculate the information about the procreation biology and make a base for genetic improvement for tetraploid Robinia pseudoacacia,the development of megaspore and microspore genesis and the formation of female and male gametophytes were researched.The results were as the followings:the anther was 4-sporangia and its anther wall formation conformed to the dicotyledonous type.And also,the tapetum was of the glandular type and its most cells were a 1-nucleate and of cytokinesis at meiosis of microspore mother cells and were simultaneous,and tetrads were tetrahedral.Besides,mature pollen grains were 2-celled.In addition,the development of embryo sac was of the polygonum type.The mature ovules were campylotropous with dual-integument and crassinucellate.The megaspore at chalazal end became a functional megaspore.There were eight nuclears in the mature embryo sac.