神农架4个珙桐居群遗传多样性的RAPD分析

RAPD analysis for the genetic diversity of four poplulations of davidia involucrata Baill.in Shengnoongjia area, hubei Province

摘要: 用随机扩增多态DNA(RAPD)技术对神农架地区4个居群28个珙桐( Davidia involurata Baill.)个体进行了遗传多样性分析.16个10 bp寡核苷酸引物共检测到87个位点，多态位点49个，多态百分率56.3% ,而居群平均多态位点百分率为30.2%. 用POPGENE对数据进行了分析，结果显示:居群的平均Nei's基因多样性为0.111 8,Shannon's遗传多样性信息指数为0.165 1，总的居群基因多样性为0.169 2,基因分化系数为0.339 2.这表明神农架地区珙桐的遗传多样性较低，其遗传变异以居群内遗传变异为主.

Abstract: Twenty-eight individuals of four Davidia involucrata Baill. populations in Shennongia area, Hubei Province were analyzed by using random amplified polymorphic DNA (RAPD) markers to determine the genetic variation among and within the populations:Eighty-seven loci were detected using sixteen 10 \mathrmbp random primers, of which 49 were exhibited polymorphism. The average percentage of polymorphic loci (PPL) was 30.2 \%, while that of polymorphic loci (PPL) was 56.3 \%. Data were analyzed by the software POPGENE and results showed that the average Nei's genetic diversity was 0.1118, Shannon's genetic diversity information index was 0.1651, genetic diversity of all populations was 0.1692 and Gst was 0.3392. The results indicate that D. involucrata in this area has relatively low genetic diversity and most of differentiation is within populations.