Structure and density distribution of wooden floor to a large extent determine its physical and mechanical properties. The internal structure of three kinds of wooden floors，including solid wood, poplar scrimber and moso bamboo scrimber，was detected using computed tomography（CT）, and their density characteristics were also presented in this paper. The density variation was poplar scrimber moso bamboo scrimber solid wood flooring; and VDP of poplar scrimber presented substantial upward trend after declining slightly from the wooden floor surface to bottom, while solid wood flooring and moso bamboo scrimber had not obvious change rules. It was found that CT could be used to detect the structure and density of wooden floor. As an advanced non-destructive technique, CT will be applicable to quality detection for wooden floor and benefit to the study on process optimization of serimber flooring.