To understand the genetic and molecular mechanisms of floral development in Populus tomentosa，a fulllength cDNA sequence of PtPCPlike gene was isolated from a male floral bud cDNA library of P.tomentosa，with the method of bacteriophage absorption by filter paperPCR.The sequencing results indicated that the 595 bp sequence contained a complete open reading frame, which encoded 91 amino acid residuals. BLAST analysis revealed that the gene had a similar sequence domain with Arabidopsis pollen coat protein genes, therefore it was named as PtPCPlike.RTPCR showed that PtPCPlike had higher expression abundance in male poplar floral buds and roots, and a lower abundance in female floral buds. A plant expression vector carrying 35S∶∶PtPCPlike was constructed and transformed into tobacco and poplar via Agrobacterium mediated method, a batch of positive transformation plants were obtained. Expression pattern of PtPCPlike in different tobacco lines was examined using qRTPCR. Results showed that PtPCPlike was expressed higher in all transgenic lines than in wide type, and significant expression differences were seen among different transgenic lines.