The molecular marker system—SRAP (sequencerelated amplified plymorphism) was used to study the genetic relationship to provide a theoretical basis for parent selection, cultivar identification and intellectual property protection of Salvia splendens. Genetic relationship of 24 S. splendens cultivars was analyzed using SRAP marker. Twentyfour primer pairs selected from 88 primer pairs amplified 306 polymorphic bands with an average of 12.8 polymorphic bands per primer pair. UPGMA clustering suggested that 24 cultivars could be divided into three groups as the genetic distance was 0.72. The first group mainly included S. splendens cultivars from Beijing Institute of Landscape Architecture, and foreign cultivars were in the second group, both ‘Vista White’ and ‘Vista Salmon’were clustered in the third group in view of different color from other flowers. In conclusion, there is good consistency between genetic diversity of germplasm resources of S. splendens using SRAP marker and germplasm sources.