Root nitrogen uptake and its relationship with root morphological and chemical traits in <i>Pinus koraiensis</i> at different ages

Ren Hao; Gao Guoqiang; Ma Yaoyuan; Li Zuwang; Gu Jiacun

doi:10.12171/j.1000-1522.20200385

Journal of Beijing Forestry University > 2021 > 43(10): 65-72. > DOI: 10.12171/j.1000-1522.20200385

Ren Hao, Gao Guoqiang, Ma Yaoyuan, Li Zuwang, Gu Jiacun. Root nitrogen uptake and its relationship with root morphological and chemical traits in Pinus koraiensis at different ages[J]. Journal of Beijing Forestry University, 2021, 43(10): 65-72. DOI: 10.12171/j.1000-1522.20200385

Citation:

PDF (833 KB)

Root nitrogen uptake and its relationship with root morphological and chemical traits in Pinus koraiensis at different ages

Key Laboratory of Sustainable Forest Ecosystem Management of Ministry of Education,School of Forestry, Northeast Forestry University, Harbin 150040, Heilongjiang, China

More Information

Received Date: December 06, 2020
Revised Date: May 11, 2021
Available Online: May 21, 2021
Published Date: October 29, 2021

Graphical Abstract

Abstract

Abstract

Objective The objective was to determine the root nitrogen uptake and its relationship with root morphological and chemical traits in Korean pine (Pinus koraiensis) at different ages, and to advance our understanding in the linkage between root resource acquisition strategy and tree ontogeny.
Method We sampled young ((14 ± 1) years), middle-aged ((48 ± 3) years) and mature ((217 ± 4) years) individuals of Korean pine in a mixed broadleaved Korean pine forest at Liangshui National Nature Reserve, Heilongjiang Province of northeastern China. Root nitrogen uptake was measured in situ with a short-term ¹⁵N labeling hydroponic experiment, and root morphological and chemical traits were also determined concurrently.
Result Root ammonium, glycine and total nitrogen uptake rate of Korean pine decreased gradually with the increase of age, while no significant change in nitrate uptake rate was found. Across all ages, the contributions of different forms of nitrogen to total uptake ranked in the order of ammonium (62%−65%) > glycine (25%−32%) > nitrate (4%−12%). The contribution of nitrate to total nitrogen uptake increased with the increase of age, but no clear patterns were shown in ammonium and glycine. The percentages of glycine as molecular absorbed by roots were very similar across young, middle-aged and mature individuals, with the corresponding values of 78%, 81% and 80%, respectively. With tree age increased, root diameter increased significantly, showing negative correlations with uptake rates of ammonium, glycine and total nitrogen (significant correlation only found in glycine), but positive correlation with nitrate. By contrast, specific root length and specific root surface area decreased with increasing age, both of them were positively correlated with uptake rates of ammonium, glycine and total nitrogen (significant correlation only found in glycine), but negatively correlated with nitrate. Root tissue density and chemical traits did not show significant changes among tree ages, and exhibited very weak relationships with nitrogen uptake rates.
Conclusion With the increase of tree age, root nitrogen uptake rate and preference of P. koraiensis are significantly changed, which may be related to the alternation of root morphological traits.
- nitrogen uptake,
- glycine,
- tree age,
- fine root,
- mixed broadleaved Korean pine forest

FullText(HTML)

References (35)

References

[1]	Lebauer D S, Treseder K K. Nitrogen limitation of net primary productivity in terrestrial ecosystems is globally distributed[J]. Ecology, 2008, 89(2): 371−379. doi: 10.1890/06-2057.1
[2]	董雯怡, 聂立水, 李吉跃, 等. 应用¹⁵N示踪研究毛白杨苗木对不同形态氮素的吸收及分配[J]. 北京林业大学学报, 2009, 31(4):97−101. doi: 10.3321/j.issn:1000-1522.2009.04.017 Dong W Y, Nie L S, Li J Y, et al. Effects of nitrogen forms on the absorption and distribution of nitrogen in Populus tomentosa seedlings using the technique of ¹⁵N tracing[J]. Journal of Beijing Forestry University, 2009, 31(4): 97−101. doi: 10.3321/j.issn:1000-1522.2009.04.017
[3]	Britto D T, Kronzucker H J. Ecological significance and complexity of N-source preference in plants[J]. Annals of Botany, 2013, 112(6): 957−963. doi: 10.1093/aob/mct157
[4]	霍常富, 孙海龙, 范志强, 等. 根系氮吸收过程及其主要调节因子[J]. 应用生态学报, 2007, 18(6):1356−1364. doi: 10.3321/j.issn:1001-9332.2007.06.032 Huo C F, Sun H L, Fan Z Q, et al. Physiological processes and major regulating factors of nitrogen uptake by plant roots[J]. Chinese Journal of Applied Ecology, 2007, 18(6): 1356−1364. doi: 10.3321/j.issn:1001-9332.2007.06.032
[5]	Liese R, Lübbe T, Albers N W, et al. The mycorrhizal type governs root exudation and nitrogen uptake of temperate tree species[J]. Tree Physiology, 2018, 38(1): 83−95. doi: 10.1093/treephys/tpx131
[6]	Hong J T, Ma X X, Yan Y, et al. Which root traits determine nitrogen uptake by alpine plant species on the Tibetan Plateau?[J]. Plant and Soil, 2018, 424(1−2): 63−72. doi: 10.1007/s11104-017-3434-3
[7]	Song M H, Zheng L L, Suding K N, et al. Plasticity in nitrogen form uptake and preference in response to long-term nitrogen fertilization[J]. Plant and Soil, 2015, 394(1−2): 215−224. doi: 10.1007/s11104-015-2532-3
[8]	Leduc S D, Rothstein D E. Plant-available organic and mineral nitrogen shift in dominance with forest stand age[J]. Ecology, 2010, 91(3): 708−720. doi: 10.1890/09-0140.1
[9]	Jagodzinski A M, Ziółkowski J, Warnkowska A, et al. Tree age effects on fine root biomass and morphology over chronosequences of Fagus sylvatica, Quercus robur and Alnus glutinosa stands[J/OL]. PLoS One, 2016, 11(2): e148668 [2020−11−16]. https://doi.org/10.1371/journal.pone.0148668.
[10]	Hishi T, Tateno R, Fukushima K, et al. Changes in the anatomy, morphology and mycorrhizal infection of fine root systems of Cryptomeria japonica in relation to stand ageing[J]. Tree Physiology, 2016, 37: 61−70.
[11]	Liu M, Xu F Z, Xu X L, et al. Age alters uptake pattern of organic and inorganic nitrogen by rubber trees[J]. Tree Physiology, 2018, 38: 1685−1693.
[12]	李常诚, 李倩茹, 徐兴良, 等. 不同林龄杉木氮素的获取策略[J]. 生态学报, 2016, 36(9):2620−2625. Li C C, Li Q R, Xu X L, et al. Nitrogen acquisition strategies of Cunninghamia lanceolate at different ages[J]. Acta Ecologica Sinica, 2016, 36(9): 2620−2625.
[13]	Zhang Z L, Li N, Xiao J, et al. Changes in plant nitrogen acquisition strategies during the restoration of spruce plantations on the eastern Tibetan Plateau, China[J]. Soil Biology and Biochemistry, 2018, 119: 50−58. doi: 10.1016/j.soilbio.2018.01.002
[14]	翟明普, 沈国舫. 森林培育学[M]. 3版. 北京: 中国林业出版社, 2016. Zhai M P, Shen G F. Silviculture [M]. 3rd ed. Beijing: China Forestry Publishing House, 2016.
[15]	Warren C R, Adams P R. Uptake of nitrate, ammonium and glycine by plants of Tasmanian wet eucalypt forests[J]. Tree Physiology, 2007, 27(3): 413−419. doi: 10.1093/treephys/27.3.413
[16]	Liu M, Li C C, Xu X L, et al. Organic and inorganic nitrogen uptake by 21 dominant tree species in temperate and tropical forests[J]. Tree Physiology, 2017, 37(11): 1515−1526. doi: 10.1093/treephys/tpx046
[17]	Lipson D, Näsholm T. The unexpected versatility of plants: organic nitrogen use and availability in terrestrial ecosystems[J]. Oecologia, 2001, 128(3): 305−316. doi: 10.1007/s004420100693
[18]	Warren C R. Potential organic and inorganic N uptake by six Eucalyptus species[J]. Functional Plant Biology, 2006, 33(7): 653−660. doi: 10.1071/FP06045
[19]	Wei L L, Chen C R, Xu Z H, et al. Direct uptake and rapid decrease of organic nitrogen by Wollemia nobilis[J]. Biology and Fertility of Soils, 2013, 49(8): 1247−1252. doi: 10.1007/s00374-013-0818-2
[20]	Guo D L, Xia M X, Wei X, et al. Anatomical traits associated with absorption and mycorrhizal colonization are linked to root branch order in twenty-three Chinese temperate tree species[J]. New Phytologist, 2008, 180(3): 673−683. doi: 10.1111/j.1469-8137.2008.02573.x
[21]	Gu J C, Xu Y, Dong X Y, et al. Root diameter variations explained by anatomy and phylogeny of 50 tropical and temperate tree species[J]. Tree Physiology, 2014, 34(4): 415−425. doi: 10.1093/treephys/tpu019
[22]	Geßler A, Kreuzwieser J, Dopatka T, et al. Diurnal courses of ammonium net uptake by the roots of adult beech (Fagus sylvatica) and spruce (Picea abies) trees[J]. Plant and Soil, 2002, 240(1): 23−32. doi: 10.1023/A:1015831304911
[23]	Mckane R B, Johnson L C, Shaver G R, et al. Resource-based niches provide a basis for plant species diversity and dominance in arctic tundra[J]. Nature, 2002, 415: 68−71. doi: 10.1038/415068a
[24]	Pregitzer K S, Deforest J L, Burton A J, et al. Fine root architecture of nine north American trees[J]. Ecological Monographs, 2002, 72(2): 293−309. doi: 10.1890/0012-9615(2002)072[0293:FRAONN]2.0.CO;2
[25]	张韫, 崔晓阳. 白桦幼苗NH₄⁺/NO₃⁻吸收特征的研究[J]. 北京林业大学学报, 2011, 33(3):26−30. Zhang Y, Cui X Y. NH₄⁺/NO₃⁻ absorption characteristics of Betula platyphylla seedlings[J]. Journal of Beijing Forestry University, 2011, 33(3): 26−30.
[26]	Zhu F F, Dai L M, Hobbie E A, et al. Uptake patterns of glycine, ammonium, and nitrate differ among four common tree species of northeast China[J]. Frontiers in Plant Science, 2019, 10: 1−11.
[27]	Rosenvald K, Ostonen I, Uri V, et al. Tree age effect on fine-root and leaf morphology in a silver birch forest chronosequence[J]. European Journal of Forest Research, 2013, 132(2): 219−230. doi: 10.1007/s10342-012-0669-7
[28]	Jagodzinski A M, Kalucka I. Fine roots biomass and morphology in a chronosequence of young Pinus sylvestris stands growing on a reclaimed lignite mine spoil heap[J]. Dendrobiology, 2010, 64: 19−30.
[29]	曾凡鹏, 迟光宇, 陈欣, 等. 辽东山区不同林龄落叶松人工林土壤−根系C∶N∶P生态化学计量特征[J]. 生态学杂志, 2016, 35(7):1819−1825. Zeng F P, Chi G Y, Chen X, et al. The stoichiometric characteristics of C, N and P in soil and root of larch (Larix spp.) plantation at different stand ages in mountainous region of eastern Liaoning Province, China[J]. Chinese Journal of Ecology, 2016, 35(7): 1819−1825.
[30]	Robinson D, Hodge A, Fitter A. Constraints on the form and function of root systems[M]//de Kroon H, Visser E J W, ed. Root ecology. Berlin: Springer, 2003: 1−31.
[31]	Kong D L, Wang J J, Zeng H, et al. The nutrient absorption-transportation hypothesis: optimizing structural traits in absorptive roots[J]. New Phytologist, 2017, 213(4): 1569−1572. doi: 10.1111/nph.14344
[32]	Guo D L, Mitchell R J, Hendricks J J. Fine root branch orders respond differentially to carbon source-sink manipulations in a longleaf pine forest[J]. Oecologia, 2004, 140(3): 450−457. doi: 10.1007/s00442-004-1596-1
[33]	Ma Z Q, Guo D L, Xu X L, et al. Evolutionary history resolves global organization of root functional traits[J]. Nature, 2018, 555: 94−97. doi: 10.1038/nature25783
[34]	Bowsher A W, Miller B J, Donovan L A. Evolutionary divergences in root system morphology, allocation, and nitrogen uptake in species from high- versus low-fertility soils[J]. Functional Plant Biology, 2016, 43(2): 129−140. doi: 10.1071/FP15162
[35]	Comas L H, Eissenstat D M. Patterns in root trait variation among 25 co-existing North American forest species[J]. New Phytologist, 2009, 182(4): 919−928. doi: 10.1111/j.1469-8137.2009.02799.x

[1]	Li Chengyu, Fang Jiaying, Wang Qihang, Zeng Lingshun, Mu Jun. Expansion pretreatment enhancing dye adsorption performance of cork biochar and its mechanism[J]. Journal of Beijing Forestry University, 2025, 47(2): 163-174. DOI: 10.12171/j.1000-1522.20240273
[2]	Yang Xin, Zhang Fangda, Huang Yanhui, Fei Benhua. Tensile and bending properties of radial slivers of Moso bamboo[J]. Journal of Beijing Forestry University, 2022, 44(3): 140-147. DOI: 10.12171/j.1000-1522.20210333
[3]	Li Jianlong, Chen Sheng, Li Haichao, Zhang Xun, Xu Duxin, Shi Menghua, Xu Feng. Relationship between cell wall ultrastructure and mechanical properties of balsa wood[J]. Journal of Beijing Forestry University, 2022, 44(2): 115-122. DOI: 10.12171/j.1000-1522.20210410
[4]	WANG Cui-cui, ZHANG Shuang-bao, XIAN Yu, WANG Dan-dan, GAO Jie, CHENG Hai-tao. Properties of plant fibers and their composites modified in situ with calcium carbonate[J]. Journal of Beijing Forestry University, 2016, 38(3): 95-101. DOI: 10.13332/j.1000-1522.20150297
[5]	GUO Kai-li, GAO Jia-rong, MA Lan, LIU Guo-hua, WANG Bing, YI Yang, WANG Shu, ZHANG Teng-fei. Distribution and tensile mechanical properties of Salix × aureo-pendula root system in soil bioengineering revetment[J]. Journal of Beijing Forestry University, 2015, 37(8): 90-96. DOI: 10.13332/j.1000-1522.20150022
[6]	DU Yu-liang, CHEN Ye, LIU Cai-hong, YIN Zeng-fang. Molecular regulation mechanism of vascular pattern formation in plant[J]. Journal of Beijing Forestry University, 2014, 36(3): 142-150. DOI: 10.13332/j.cnki.jbfu.2014.03.023
[7]	TIAN Gen-lin, JIANG Ze-hui, YU Yan, WANG Han-kun, AN Xiao-jing. Toughness mechanism of bamboo by insitu tension.[J]. Journal of Beijing Forestry University, 2012, 34(5): 144-147.
[8]	ZHANG Shuang-yan, FEI Ben-hua, YU Yan, CHENG Hai-tao, WANG Chuan-gui. Influence of lignin content on tensile properties of single wood fiber.[J]. Journal of Beijing Forestry University, 2012, 34(1): 131-134.
[9]	WANG Ge, CHEN Hong, YU Yan, CHENG Hai-tao, TIAN Gen-lin, CHEN Xiao-meng. Fine characterization techniques of physical and mechanical properties of bamboo fiber in cell level.[J]. Journal of Beijing Forestry University, 2011, 33(4): 143-148.
[10]	MENG Xi, WANG Ruo-han, XIE Lei, LONG Ru, MOU Shu-lin, ZHANG Zhi-xiang. Flowering dynamics and dichogamous mechanism in Magnolia grandiflora[J]. Journal of Beijing Forestry University, 2011, 33(4): 63-69.

Cited By

Cited by

Periodical cited type(13)

1.	聂靖，陆驰，欧光龙，胥辉. 基于Landsat8 OLI遥感因子的思茅松地上生物量二阶抽样估测. 林业资源管理. 2022(06): 68-75 .
2.	阳帆，白星雯. 森林资源监测地面固定样地优化研究. 林业资源管理. 2022(06): 76-81 .
3.	王伟，杨净，高显连，曾伟生. 2020年全球森林资源评估遥感调查方法和思考. 林业资源管理. 2021(06): 1-5 .
4.	曹飞，穆宝慧，徐丹，高乾，孙建欣，孙浩，孙中平. 遥感技术在环境变化监测中的应用进展. 环境与可持续发展. 2020(02): 96-99 .
5.	辛成锋. 新一轮森林资源二类调查技术要点——以广东省茂名地区为例. 湖南林业科技. 2019(02): 72-76 .
6.	马炜，张阳武，周天元，蒋亚芳. 基于空间抽样调查的宁夏全区和吴忠市湿地面积估测. 湿地科学. 2019(04): 384-390 .
7.	刘谦，张煜星，王雪军，王少杰，杨英，I Nengah Suratijaya，Dewayany Sutrisno，Ita Carolita. 东南亚国家森林资源年度遥感监测设计——以印度尼西亚为例. 林业资源管理. 2018(03): 113-120 .
8.	蒋仟，林辉，严恩萍，罗攀. 基于SPOT5遥感影像分类的抽样技术研究. 西南林业大学学报(自然科学). 2018(03): 145-150 .
9.	陈宗铸，杨琦，雷金睿，陈小花，李苑菱. 基于激光雷达数据的热带森林冠高模型生成及平均树高估计. 中南林业科技大学学报. 2018(07): 1-7 .
10.	张煜星，王雪军，黄国胜，党永峰，陈新云. 森林面积多阶遥感监测方法. 林业科学. 2017(07): 94-104 .
11.	陆月报. 提高森林采伐调查设计精度和效率探讨. 农技服务. 2017(06): 93-94 .
12.	葛宏立，孟源源. 森林面积不同抽样估计方法的无偏性及有效性分析与证明. 林业资源管理. 2016(04): 47-52 .
13.	孟源源，葛宏立. 块状与带状森林的面积抽样估计计算机模拟. 林业资源管理. 2016(02): 49-55 .

Other cited types(9)

Get Citation

PDF

XML

Article views (1286) PDF downloads (142) Cited by(22)

Turn off MathJax

Article Contents

Abstract

References

Root nitrogen uptake and its relationship with root morphological and chemical traits in Pinus koraiensis at different ages