• Scopus
  • Chinese Science Citation Database (CSCD)
  • A Guide to the Core Journal of China
  • CSTPCD
  • F5000 Frontrunner
  • RCCSE
Advanced search
Chen Xiaomeng, Wang Yuechen, Chai Xuying, Li Aining, Wang Yonglin. Deletion mutations and its phenotypic analysis of two-component genes in Lonsdalea populi[J]. Journal of Beijing Forestry University, 2021, 43(9): 25-37. DOI: 10.12171/j.1000-1522.20210007
Citation: Chen Xiaomeng, Wang Yuechen, Chai Xuying, Li Aining, Wang Yonglin. Deletion mutations and its phenotypic analysis of two-component genes in Lonsdalea populi[J]. Journal of Beijing Forestry University, 2021, 43(9): 25-37. DOI: 10.12171/j.1000-1522.20210007

Deletion mutations and its phenotypic analysis of two-component genes in Lonsdalea populi

More Information
  • Received Date: January 07, 2021
  • Revised Date: April 04, 2021
  • Available Online: April 11, 2021
  • Published Date: October 14, 2021
  •   Objective  The bacterial canker of Populus euramericana is caused by the Gram-negative bacterium Lonsdalea populi. The rapid spread of the disease has seriously threatened the growth and development of P. euramericana and has caused great economic losses to the plantation. Two-component system (TCS) is the important signal transduction mechanism of Lonsdalea populi. Now, how the two-component system of poplar bacterial canker regulates the pathogenic process is still lack of systematic research. Therefore, the large-scale deletion mutation and mutant phenotype analysis of TCS in this study will provide genetic materials for further study on the pathogenic mechanism of poplar bacterial canker.
      Method  In this study, 28 two-component gene deletion mutants of poplar canker pathogen strain L. populi N-5-1 were constructed by parental association, the differences in pathogenicity, growth, motility, biofilm formation and resistance of these mutants were analyzed by phenotypic analysis, and the regulation of two-component system coding genes on the pathogenicity of these mutants was studied.
      Result  In this study, 36 recombinant vectors of two-component coding genes were constructed and 28 deletion mutants were obtained. Phenotypic analysis showed that 18 genes encoding TCS were involved in virulence of L. populi N-5-1. Among them, the pathogenicity of 8 mutants had obviously disappeared. In addition, the deletion mutants regulating motility and biofilm-forming ability and those deficient in stress response (metal ions, salt ions, antibiotic stress, etc.) were also screened.
      Conclusion  In this study, five two-component genes significantly affecting the pathogenicity of L. populi were obtained, providing genetic material for future studies on the pathogenic mechanism of L. populi.
  • [1]
    贺伟, 任飞娟, 郭利民, 等. 欧美杨溃疡病的病原鉴定[J]. 林业科学, 2009, 45(6):104−108, 181. doi: 10.3321/j.issn:1001-7488.2009.06.018

    He W, Ren F J, Guo L M, et al. Pathogen identification of poplar canker[J]. Forestry Science, 2009, 45(6): 104−108, 181. doi: 10.3321/j.issn:1001-7488.2009.06.018
    [2]
    Li Y, He W, Ren F, et al. A canker disease of Populus × euramericana in China caused by Lonsdalea quercina subsp. populi[J]. Plant Disease, 2014, 98: 368−378. doi: 10.1094/PDIS-01-13-0115-RE
    [3]
    Toth T, Lakatos T, Koltay A. Lonsdalea quercina subsp. populi subsp nov., isolated from bark canker of poplar trees[J]. International Journal of Systematic and Evolutionary Microbiology, 2013, 63: 2309−3101. doi: 10.1099/ijs.0.042911-0
    [4]
    Jansson S, Douglas C J. Populus: a model system for plant biology[J]. Annual Review Plant Biology, 2007, 58: 435−458. doi: 10.1146/annurev.arplant.58.032806.103956
    [5]
    Schaller G E. Histidine kinases and the role of two-component systems in plants[J]. Advances in Botanical Research, 2000, 32: 109−148.
    [6]
    West A H, Stock A M. Histidine kinases and response regulator proteins in two-component signaling systems[J]. Tends in Biochemical Sciences, 2001, 26: 369−376. doi: 10.1016/S0968-0004(01)01852-7
    [7]
    Tang J, Liu Y N, Barber C E, et al. Genetic and molecular analysis of a cluster of rpf genes involved in positive regulation of synthesis of extracellular enzymes and polysaccharide in Xanthomonas campestris pathovar campestris[J]. Molecular & General Genetics, 1991, 226(3): 409−417.
    [8]
    Fishman M R, Zhang J, Bronstein P A, et al. Ca2+ -induced two-component system CvsSR regulates the type III secretion system and the extracytoplasmic function sigma factor AlgU in Pseudomonas syringae pv. tomato DC3000[J/OL]. Journal of Bacteriology, 2018, 200: e00538−17 [2021−01−13]. https://jb.asm.org/content/200/5/e00538-17.
    [9]
    Xia C, Jian Z, Ming L C, et al. The effect of the potential PhoQ his tidine kinase inhibitors on Shigella flexneri virulence[J/OL]. PLoS ONE, 2011, 6(8): e23100 [2021−01−14]. https://doi.org/110.1371/journal.pone.0023100.
    [10]
    Yang F, Tian F, Sun L, et al. A novel two-component system PdeK/PdeR regulates c-di-GMP turnover and virulence of Xanthomonas oryzae pv. oryzae[J]. Mol Plant Microbe Interact, 2012, 25(10): 1361−1369. doi: 10.1094/MPMI-01-12-0014-R
    [11]
    Yang R L, Deng C Y, Wei J W, et al. A large-scale mutational analysis of two-component signaling systems of Lonsdalea quercina revealed that KdpD-KdpE regulates bacterial virulence against host poplar trees[J]. Molecular Plant-Microbe Interactions, 2018, 31: 724−736. doi: 10.1094/MPMI-10-17-0248-R
    [12]
    Zheng Z, Deng C, He W, et al. The two-component system DcuS-DcuR is involved in virulence and stress tolerance in the poplar canker bacterium Lonsdalea populi[J]. Phytopathology, 2020, 110(10): 1763−1772.
    [13]
    郑泽洋, 李爱宁, 常聚普, 等. 欧美杨细菌性溃疡病菌双组分系统lqp0812-lqp0813基因功能研究[J]. 植物病理学报, 2020, 50(3):301−310.

    Zheng Z Y, Li A N, Chang J P, et al. Functional analysis of two-component lqp0812-lqp0813 gene in poplar bacterial canker[J]. Plant Pathology, 2020, 50(3): 301−310.
    [14]
    Nowak A, Tyski S. The role of two-component regulatory systems of Gram-positive cocci in biofilm formation[J]. Postepy Mikrobiologii, 2012, 51: 265−276.
    [15]
    Kravchenko U, Gogoleva N, Kalubaka N, et al. The PhoPQ two-component system is the major regulator of cell surface properties, stress responses and plant-derived substrate utilisation during development of Pectobacterium versatile-host plant pathosystems[J/OL]. Frontiers in Microbiology, 2021: 1: 621391 [2021−01−19]. https://doi.org/10.3389/fmicb.2020.621391.
    [16]
    Sébastien B G, Edwige M, Lacroix J M. The two-component system CpxAR is essential for virulence in the phytopathogen bacteria Dickeya dadantii EC3937[J]. Environmental Microbiology, 2016, 17(11): 4415−4428.
    [17]
    Gooderham W J, Hancock R E W. Regulation of virulence and antibiotic resistance by two-component regulatory systems in Pseudomonas aeruginosa[J]. Fems Microbiology Reviews, 2009, 33: 279−294. doi: 10.1111/j.1574-6976.2008.00135.x
    [18]
    Baek J. Transcriptome analysis of phosphate starvation response in Escherichia coli[J]. Journal of Microbiology & Biotechnology, 2007, 17(2): 244.
    [19]
    Howery K E, ClemMer K M, Rather P N. The Rcs regulon in Proteus mirabilis: implications for motility, biofilm formation, and virulence[J]. Current Genetics, 2016, 62: 775−789. doi: 10.1007/s00294-016-0579-1
    [20]
    Wang Q, Zhao Y, Mcclelland M, et al. The RcsCDB signaling system and swarming motility in Salmonella enterica serovar : dual regulation of flagellar and SPI-2 virulence genes[J]. Journal of Bacteriology, 2007, 189: 8447−8457. doi: 10.1128/JB.01198-07
    [21]
    Alon U. Response regulator output in bacterial chemotaxis[J]. The EMBO Journal, 1998, 17(15): 4238−4248. doi: 10.1093/emboj/17.15.4238
    [22]
    Bertrand J J, West J T, Engel J N. Genetic analysis of the regulation of type IV pilusfunction by the Chp chemosensory system of Pseudomonas aeruginosa[J]. Journal of Bacteriology, 2010, 192: 994−1010. doi: 10.1128/JB.01390-09
    [23]
    Blus-Kadosh I, Zilka A, Yerushalmi G, et al. The effect of pstS and phoB on quorum sensing and swarming motility in Pseudomonas aeruginosa[J/OL]. PLoS One, 2013, 8(9): e74444 [2021−01−06]. https//joumals.plos.org/pkosone/article?id=10.1371/journal.pone.0074444.
    [24]
    Zhang B, Zhang Y, Liang F, et al. An extract produced by Bacillus sp. BR3 influences the function of the GacS/GacA two-component system in Pseudomonas syringae pv. tomato DC3000[J]. Frontiers in Microbiology, 2019, 10: 2005. doi: 10.3389/fmicb.2019.02005
    [25]
    Bereswill S, Geider K. Characterization of the rcsB gene from Erwinia amylovora and its influence on exoploysaccharide synthesis and virulence of the fire blight pathogen[J]. Journal of Bacteriology, 1997, 179(4): 1354−1361. doi: 10.1128/JB.179.4.1354-1361.1997
    [26]
    Birgit M P. Involvement of two-component signaling on bacterial motility and biofilm development[J/OL]. Journal of Bacteriology, 2017, 199(18): e00259−17 [2020−12−16]. https://jb.asm.org/content/199/18/e00259-17.
    [27]
    Goulart C L, Barbosa L C, Bisch P M, et al. Catalases and PhoB/PhoR system independently contribute to oxidative stress resistance in Vibrio cholerae O1[J]. Microbiology, 2016, 162(11): 1955−1962. doi: 10.1099/mic.0.000364
    [28]
    Tran T K, Han Q Q, Shi Y, et al. A comparative proteomic analysis of Salmonella typhimurium under the regulation of the RstA/RstB and PhoP/PhoQ systems[J]. Biochimica et Biophysica Acta, 2016, 1864(12): 1686−1695. doi: 10.1016/j.bbapap.2016.09.003
    [29]
    Hu L Z, Zhang W P, Zhou M T, et al. Analysis of Salmonella PhoP/PhoQ regulation by dimethyl-SRM-based quantitative proteomics[J]. Biochimica et Biophysica Acta, 2016, 1864(1): 20−28. doi: 10.1016/j.bbapap.2015.10.003
    [30]
    Zheng D, Xue B, Shao Y, et al. Activation of PhoBR under phosphate-rich conditions reduces the virulence of Xanthomonas oryzae pv. Oryza[J]. Molecular Plant Pathology, 2018, 19(9): 2066−2076. doi: 10.1111/mpp.12680
  • Related Articles

    [1]Li Zhu, Jiang Jiali, Lü Jianxiong. Tensile creep characteristics of compression wood and normal wood under different temperatures and loads[J]. Journal of Beijing Forestry University, 2024, 46(12): 138-145. DOI: 10.12171/j.1000-1522.20240277
    [2]Wu Haoyang, Niu Jianzhi, Wang Di, Qiu Qihuang, Yang Tao, Yang Shujian. Characteristics of the macropore structure of ice-marginal landforms in the Liaodong Mountain Area of northeastern China and its influence on soil aggregate stability and soil erodibility[J]. Journal of Beijing Forestry University, 2023, 45(6): 69-81. DOI: 10.12171/j.1000-1522.20220283
    [3]LI Jin-ke, DENG Wen-hong, CHEN Shao-liang. Gel permeation chromatography (GPC)high performance liquid chromatographic (HPLC) determination of cytokinin in plant tissues.[J]. Journal of Beijing Forestry University, 2012, 34(6): 155-159.
    [4]QIU Er-fa, XU Fei, WANG Cheng, DONG Jian-wen, WU Yong-shu, . Population distribution and structure characteristics of village roadside forest in Fujian Province, eastern China[J]. Journal of Beijing Forestry University, 2012, 34(6): 68-74.
    [5]KONG Ying, SUN Ming, PAN Hu-tang, ZHANG Qi-xiang. Advances in metabolism and regulation of floral scent.[J]. Journal of Beijing Forestry University, 2012, 34(2): 146-154.
    [6]NIAN Hong-li, LI He, CAO Dong-dong, CAO Jian-kang, JIANG Wei-bo. Determination of phenolic compounds in jujube peels at different maturity stages by high performance liquid chromatography.[J]. Journal of Beijing Forestry University, 2011, 33(1): 139-143.
    [7]WANG Wei, ZHENG Xiao-xian, NING Yang-cui. Structural characteristics of typical water conservation forests in mountain areas of Beijing.[J]. Journal of Beijing Forestry University, 2011, 33(1): 60-63.
    [8]HU Xiao-dan, ZHANG De-quan, SUN Ai-dong, WANG Jian-zhong, LIU Yu-jun. Separation of perilla ketone by high speed countercurrent chromatography[J]. Journal of Beijing Forestry University, 2007, 29(5): 170-172. DOI: 10.13332/j.1000-1522.2007.05.031
    [9]GAO Lin, WANG Nai-kang, GAO Yong. Fuzzy control on air-suction seeding system in the seedling production line[J]. Journal of Beijing Forestry University, 2007, 29(4): 75-79. DOI: 10.13332/j.1000-1522.2007.04.018
    [10]CHANG De-long, SONG Zhan-qian, HUANG Wen-hao, HU Wei-hua, LI Fu-hai, ZHANG Quan-lai. Impacts of fungi on chemical components and physical structure of Paulownia elongata wood[J]. Journal of Beijing Forestry University, 2006, 28(3): 145-149.
  • Cited by

    Periodical cited type(17)

    1. 高杰. 天然林保护对生态系统服务功能的影响. 农业灾害研究. 2024(02): 232-234 .
    2. 力佳琪,麦强盛,王俊超. 玉白顶自然保护区森林生态价值评估. 农业与技术. 2024(18): 67-71 .
    3. 潘丰十,牛香,郭珂. 呼伦贝尔市典型生态产品禀赋与价值化实现路径优化. 林业科学. 2024(12): 146-157 .
    4. 严雨桐,陈花丹,游巍斌,刘进山,蔡昌棠,何东进. 基于能值分析的天宝岩泥炭沼泽生态系统服务价值评估. 生态与农村环境学报. 2023(03): 335-343 .
    5. 李保杰,褚帅,顾和和. 淮海经济区生态系统服务价值时空分异特征研究. 地域研究与开发. 2023(02): 167-172 .
    6. 魏媛,吴长勇,洪林. 碳中和导向下贵州省森林资源生态价值评估及生态补偿研究. 自然资源情报. 2023(04): 44-50 .
    7. 赵玉堂. 普达措国家公园森林生态系统服务价值评估与分析. 林业调查规划. 2023(03): 208-213 .
    8. 邓紫君,刘鑫,祖浩然,苏闪闪,陈颖,罗俊毅,闫文德,张翔,王明旭. 湖南省森林型国家级自然保护区森林生态系统服务功能价值评估. 湖南林业科技. 2023(04): 72-80 .
    9. 李连强,杨会侠,丁国泉,李虹谕,白荣芬,王品. 辽宁仙人洞国家级自然保护区森林生态服务物质量评估及权衡与协同. 北京林业大学学报. 2023(09): 83-94 . 本站查看
    10. 白晓航,施佳颖. 黑龙江丰林国家级自然保护区红松+紫椴+硕桦群系优势树种生态位特征与种间联结分析. 园林. 2023(10): 14-21 .
    11. 李超,谢飞,苏学威,罗传文. 凉水国家级自然保护区森林生态系统服务功能评估. 中国林副特产. 2023(06): 17-18 .
    12. 党俊. 移植栽培技术在自然保护区天然林保护工程生态修复中的应用. 环境保护与循环经济. 2023(12): 68-71 .
    13. 张颖,刘平辉,朱传民,张林颖. 基于NPP的抚州市生态系统服务功能重要性评价. 贵州农业科学. 2022(02): 133-140 .
    14. 胡建忠. 对我国系统种植开发沙棘的回顾与建议. 防护林科技. 2022(04): 75-77 .
    15. 王晓康. 山西省关帝山国有林区森林生态系统服务功能价值估算研究. 中国农学通报. 2022(23): 49-55 .
    16. 任志华,秦磊. 黑龙江省乡村振兴战略实施下的乡村发展策略. 规划师. 2022(09): 139-144 .
    17. 张卫民. 中国自然保护地生态资产核算框架研究. 自然保护地. 2021(02): 22-30 .

    Other cited types(15)

Catalog

    Article views (1189) PDF downloads (68) Cited by(32)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return