Physiological adaptation mechanism of 4-age wintering larvae of <i>Monochamus alternatus</i> (Coleoptera: Cerambycidae) in response to low temperature stress

Liu Jiaqi; Zhao Lijuan; Liu Lei; Huang Ruifen; Han Youzhi; Zhang Zhiwei; Cui Shaopeng; Gao Ruihe

doi:10.12171/j.1000-1522.20220438

Journal of Beijing Forestry University > 2024 > 46(4): 74-83. > DOI: 10.12171/j.1000-1522.20220438

Liu Jiaqi, Zhao Lijuan, Liu Lei, Huang Ruifen, Han Youzhi, Zhang Zhiwei, Cui Shaopeng, Gao Ruihe. Physiological adaptation mechanism of 4-age wintering larvae of Monochamus alternatus (Coleoptera: Cerambycidae) in response to low temperature stress[J]. Journal of Beijing Forestry University, 2024, 46(4): 74-83. DOI: 10.12171/j.1000-1522.20220438

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Physiological adaptation mechanism of 4-age wintering larvae of Monochamus alternatus (Coleoptera: Cerambycidae) in response to low temperature stress

Liu Jiaqi^{1, 2,},
Zhao Lijuan^{1, 2},
Liu Lei^{1, 2},
Huang Ruifen³,
Han Youzhi¹,
Zhang Zhiwei^{1, 2},
Cui Shaopeng^{1, 2},
Gao Ruihe^{1, 2, ,}

1.
College of Forestry, Shanxi Agricultural University, Taigu 030800, Shanxi, China
2.
Shanxi Dangerous Forest Pest Inspection and Identification Center, Taigu 030800, Shanxi, China
3.
Biological Disaster Prevention and Control Center of National Forestry andGrassland Administration, Shenyang 110034, Liaoning, China

More Information

Received Date: October 31, 2022
Revised Date: May 31, 2023
Accepted Date: August 10, 2023
Available Online: August 15, 2023

Graphical Abstract

Abstract

Abstract

Objective
This paper aims to determine the physiological adaptation mechanism of the 4-age wintering larva of M. alternatus to low temperature stress.
Method
The 4-age wintering larva of M. alternatus were collected from Dexing, Jiangxi Province of eastern China in mid-March of the 2021, and seven temperature gradients of 25 ℃ (control), 4, 0, −5, −10, −15 and −20 ℃ and three time gradients of 30 min, 1 h and 3 h were set, the cold tolerance, content of cold-resistant substances, activity of antioxidant enzymes and the damage of midgut under low temperature stress were determined.
Result
(1) Near the supercooling point (−9.27 ± 0.90) ℃, the 50% lethal time was about 1.9 h (114.97 min), and the 99% lethal time was about 2.8 d (4 061.86 min). Under low temperature stress below the supercooling point (−15 ℃), the lethal time of 50% and 99% was about 1/4 of that under low temperature treatment of −10 ℃. (2) After low temperature stress treatment, the water content of the 4-age wintering larvae did not change significantly, the fat content and glycogen mass concentration were significantly decreased after 3 h at 0 ℃ (P < 0.05), and the sorbitol mass concentration was significantly increased after 1 h at −5 ℃ (P < 0.05), and significantly decreased after 1 h at −20 ℃ (P < 0.05). (3) After low temperature stress treatment, the activities of antioxidant enzymes (SOD, POD, CAT) were significantly increased (P < 0.05). (4) The midgut of the 4-age wintering larvae of M. alternatus was decomposed after −15 ℃ treatment for 3 h, and vacuoles appeared between cells. After −20 ℃ treatment for 3 h, the midgut cells were ruptured, and the cell vacuoles escaped and filled the whole intestinal cavity, and the vacuoles disappeared.
Conclusion
This study clarified the physiological response of oxidative stress of the fourth instar overwintering larvae to low temperature stress, which could enrich and improve the study on cold tolerance mechanism of M. alternatus.
- Monochamus alternatus,
- wintering larvae,
- cold resistant substances,
- antioxidant enzyme system

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References (50)

References

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Physiological adaptation mechanism of 4-age wintering larvae of Monochamus alternatus (Coleoptera: Cerambycidae) in response to low temperature stress