Extraction of total flavonoids from <i>Cronartium orientale</i> aeciospores by response surface methodology and its antioxidant activity in vitro

WENG Yue-tai; XUE Yu; XU Shuo; ZHANG Guo-liang; LIU Yue; DI Xue-ying

doi:10.13332/j.1000-1522.20160269

Journal of Beijing Forestry University > 2017 > 39(3): 38-47. > DOI: 10.13332/j.1000-1522.20160269

WENG Yue-tai, XUE Yu, XU Shuo, ZHANG Guo-liang, LIU Yue, DI Xue-ying. Extraction of total flavonoids from Cronartium orientale aeciospores by response surface methodology and its antioxidant activity in vitro[J]. Journal of Beijing Forestry University, 2017, 39(3): 38-47. DOI: 10.13332/j.1000-1522.20160269

Citation:

PDF (5625 KB)

Extraction of total flavonoids from Cronartium orientale aeciospores by response surface methodology and its antioxidant activity in vitro

School of Forestry, Northeast Forestry University, Harbin, Heilongjiang, 150040, P. R. China

More Information

Received Date: August 26, 2016
Revised Date: November 16, 2016
Published Date: February 28, 2017

Graphical Abstract

Abstract

Abstract

To explore the optimum extraction process of total flavoniods from Cronartium orientale aeciospores and evaluate the antioxidant activity, ethanol solution was used to extract the total flavonoids and NaNO_2-Al(NO₃)₃ method was established to measure the total flavonoids rutin equivalent. Based on the single-factor tests, the extraction conditions of total flavonoids from Cronartium orientale aeciospores were optimized by response surface methodology. Meanwhile, Vitamin C was used to be a positive control on assaying and evaluating bioactivities of the total flavonoids, including total antioxidant capacity, total reducing power, capacities of scavenging 1, 1-diphenyl-2-picrylhydrazyl free radicals, scavenging hydroxyl free radical, and scavenging superoxide anion. The results showed that: 1)The optimum extraction conditions were as follows: extraction temperature 65℃, solid-liquid ratio 1:120, extraction time 38min, ethanol concentration 60%. Under the optimal conditions, the extraction rate of total flavonoids was 1.382%, which was close to the predicted value 1.416%, with 2.46% deviation. 2) In addition, total flavonoids from Cronartium orientale aeciospores possessed high total antioxidant capacity like Vc. 3) The reducing power of the total flavonoids and dose-effect relationship between reducing power and concentration were higher and clearer than Vc. 4) The total flavonoids on DPPH· scavenging capacity was lower than Vc, and the IC₅₀ on DPPH· scavenging of the flavonoids and Vc were 0.0492 and 0.0335mg/mL, respectively. 5) The flavonoids on ·OH scavenging was significant, much higher than Vc, and the IC₅₀ was 0.0129mg/mL. When the concentration was 0.0235mg/mL, the clearance could reach up to 84.94%. 6) O^2-· scavenging capacity of the total flavonoids is still unknown by xanthine oxidase method because of the interference factor in extraction solution. Reliable extraction conditions were obtained by response surface methodology and excellent antioxidant activity in vitro of the total flavonoids was verified by comparing with Vc. This could provide theoretical bases for development and utilization of rust fungi and changing concept of forest pest control.
- Cronartium orientale aeciospore,
- total flavonoids,
- free radical,
- antioxidation

FullText(HTML)

References (25)

References

[1]	薛煜, 邵力平, 马泽芳, 等.兴凯湖松瘤锈病病、健组织中主要有机成分变化及其对结实的影响[J].东北林业大学学报, 1992, 20(2): 30-34. http://www.cnki.com.cn/Article/CJFD1992-DBLY199202004.htm XUE Y, SHAO L P, MA Z F, et al. Change in main organic matter contents between infected and healthy tissues and the effect of Xinkai Lake pine rust to fruiting ability[J].Journal of Northeast Forestry University, 1992, 20(2): 30-34. http://www.cnki.com.cn/Article/CJFD1992-DBLY199202004.htm
[2]	KANEKO S. Cronartium orientale, sp. nov., segregation of the pine gall rust in eastern Asia from Cronartium quercuum[J]. Mycoscience, 2000, 41(2): 115-122. doi: 10.1007/BF02464319
[3]	邵力平, 薛煜, 王振华, 等.兴凯湖松瘤锈病损失量的研究[J].东北林业大学学报, 1989, 17(2): 1-5. http://d.old.wanfangdata.com.cn/Conference/280810 SHAO L P, XUE Y, WANG Z H, et al. Study on the losses of Xingkai Lake pine gall rust[J].Journal of Northeast Forestry University, 1989, 17(2): 1-5. http://d.old.wanfangdata.com.cn/Conference/280810
[4]	PIETTA P G. Flavonoids as antioxidants[J]. Journal of Natural Products, 2000, 63(7): 1035-1042. doi: 10.1021/np9904509
[5]	RICE-EVANS C. Flavonoids antioxidants[J]. Current Medicinal Chemistry, 2001, 8(7): 797-807. doi: 10.2174/0929867013373011
[6]	BIRT D F, JEFFERY E. Flavonoids[J]. Advances in Nutrition: An International Review Journal, 2013, 4(5): 576-577. doi: 10.3945/an.113.004465
[7]	YAO L H, JIANG Y M, SHI J, et al. Flavonoids in food and their health benefits[J]. Plant Foods for Human Nutrition, 2004, 59(3): 113-122. doi: 10.1007/s11130-004-0049-7
[8]	RAMAN S T, GANESHAN A K, CHEN C, et al. In vitro and in vivo antioxidant activity of flavonoid extracted from mulberry fruit (Morus alba L.)[J]. Pharmacognosy Magazine, 2016, 12(46): 128-133. doi: 10.4103/0973-1296.177910
[9]	NAEEM I, TASKEEN A, MUBEEN H, et al. Characterization of flavonols present in barks and needles of Pinus wallichiana and Pinus roxburghii[J]. Asian Journal of Chemistry, 2010, 22(1): 41-44. https://www.researchgate.net/publication/281535179_Characterization_of_Flavonols_Present_in_Barks_and_Needles_of_Pinus_wallichiana_and_Pinus_roxburghii
[10]	MAIMOONA A, NAEEM I, SADDIQE Z, et al. Analysis of total flavonoids and Phenolics in different fractions of bark and needles of Pinus roxburghii and Pinus wallichiana [J].Journal of Medicinal Plant Research, 2011, 5(21):5216-5220.
[11]	冯慧英, 樊金拴, 刘滨, 等.巴山冷杉黄酮的提取鉴定及其抗氧化性分析[J].食品工业, 2016, 37(1): 63-67. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=spgy201601016 FENG H Y, FAN J S, LIU B, et al.Extraction and identification of flavonoids from Abies fargesii and its antioxidant activity[J].The Food Industry, 2016, 37(1): 63-67. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=spgy201601016
[12]	李石清, 张春椿, 年慧慧, 等.南方红豆杉枝叶中黄酮组分的含量测定及抗氧化活性研究[J].甘肃中医学院学报, 2012, 29(3): 28-31. doi: 10.3969/j.issn.1003-8450.2012.03.010 LI S Q, ZHANG C C, NIAN H H, et al. Study on antioxidant activity and determination of different flavonoids components in branches and leaves of Taxus mairei (Lemee et Levl.)[J].Journal of Gansu University College of Traditional Chinese Medicine, 2012, 29(3): 28-31. doi: 10.3969/j.issn.1003-8450.2012.03.010
[13]	王飞, 樊金拴, 冯慧英, 等.青扦针叶总黄酮超声提取及抗氧化活性[J].西北林学院学报, 2016, 31(1): 243-249. doi: 10.3969/j.issn.1001-7461.2016.01.42 WANG F, FAN J S, FENG H Y, et al. Ultrasound assisted extraction and antioxidant activities of total flavonoids in the needles of Picea wilsonii[J].Journal of Northwest Forestry University, 2016, 31(1): 243-249. doi: 10.3969/j.issn.1001-7461.2016.01.42
[14]	KOZARSKI M S, KLAUS A S, NIKSIC M P, et al. Polysaccharides of higher fungi: biological role, structure and antioxidative activity[J]. Hemijska Industrija, 2014, 68(3): 305-320. doi: 10.2298/HEMIND121114056K
[15]	张文州, 许嵘.食药用真菌多糖的研究进展[J].食品工业科技, 2014, 35(15): 395-399. http://d.old.wanfangdata.com.cn/Periodical/smdhx200202022 ZHANG W Z, XU R. Research progress in edible-medicinal fungi polysaccharide[J].Science and Technology of Food Industry, 2014, 35(15): 395-399. http://d.old.wanfangdata.com.cn/Periodical/smdhx200202022
[16]	FU L, XU B, XU X, et al. Antioxidant capacities and total phenolic contents of 56 wild fruits from south China[J]. Molecules, 2010, 15(12): 8602-8617. doi: 10.3390/molecules15128602
[17]	FREINBICHLER W, BIANCHI L, COLIVICCHI M A, et al. The detection of hydroxyl radicals in vivo[J]. Journal of Inorganic Biochemistry, 2008, 102(5-6): 1329-1333. doi: 10.1016/j.jinorgbio.2007.12.017
[18]	陈士明, 陆亚蒙, 严小敏, 等.超氧阴离子自由基的产生及其与天然药物的作用[J].复旦学报(自然科学版), 1991, 30(1): 31-36. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=HY000001731385 CHEN S M, LU Y M, YAN X M, et al. Generation of superoxide anion radical and the effect on natural medicines[J].Journal of Fudan University(Natural Science), 1991, 30(1): 31-37. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=HY000001731385
[19]	ROBAK J, GRYGLEWSKI R J. Flavonoids are scavengers of superoxide anions[J]. Biochemical Pharmacology, 1988, 37(5): 837-841. doi: 10.1016/0006-2952(88)90169-4
[20]	PUPPO A, RIGAUD J, JOB D. Role of superoxide anion in leghemoglobin autoxidation[J]. Plant Science Letters, 1981, 22(4): 353-360. doi: 10.1016/0304-4211(81)90081-X
[21]	闫琳娜.松针落叶总黄酮的提取、纯化及抗氧化性能分析[D].吉林: 吉林大学, 2009. YAN L N. Extraction, purification and antioxidant activity analysis of flavonoids from pine needle[D]. Jilin: Jilin University, 2009.
[22]	孙琼.杏鲍菇黄酮类化合物提纯及抗氧化活性研究[D].临汾: 山西师范大学, 2015. SUN Q.Study on extraction and purification process of flavonoids from Pleurotus eryngii and its antioxidant activities[D]. Linfen: Shanxi Normal University, 2015.
[23]	黄晓冬, 蔡建秀, 许丽璇, 等.桦褐孔菌总黄酮体内外抗氧化作用[J].食品与生物技术学报, 2012, 31(10): 1062-1068. doi: 10.3969/j.issn.1673-1689.2012.10.009 HUANG X D, CAI J X, XU L X, et al.Study on antioxidation effect in vitro and in vivo of total flavonoids from Inonotus obliquus[J].Journal of Food Science and Biotechnology, 2012, 31(10): 1062-1068. doi: 10.3969/j.issn.1673-1689.2012.10.009
[24]	BOONSONG S, KLAYPRADIT W, WILAIPUN P. Antioxidant activities of extracts from five edible mushrooms using different extractants[J]. Agriculture and Natural Resources, 2016, 50(2): 89-97. doi: 10.1016/j.anres.2015.07.002
[25]	TREML J, ŠMEJKAL K. Flavonoids as potent scavengers of hydroxyl radicals[J]. Comprehensive Reviews in Food Science and Food Safety, 2016, 15(4): 720-738. doi: 10.1111/1541-4337.12204

[1]	Fu Yu, Shi Xuan, Zhang Daoming, Zhang Xiyu, Fu Qun. Antioxidant activities in extracts from Pinus massoniana needles by deep eutectic solvents[J]. Journal of Beijing Forestry University, 2021, 43(7): 149-158. DOI: 10.12171/j.1000-1522.20200030
[2]	Yao Kun, Lian Conglong, Wang Jingjing, Wang Houling, Liu Chao, Yin Weilun, Xia Xinli. PePEX11 functions in regulating antioxidant capacity of Arabidopsis thaliana under salt stress[J]. Journal of Beijing Forestry University, 2018, 40(5): 19-28. DOI: 10.13332/j.1000-1522.20180086
[3]	FAN Zi-luan, ZHANG Yan-dong, ZHANG Hua, WANG Zhen-yu, BAO Yi-hong. Antioxidant and antibacterial activity of essential oil from Pinus koraiensis needles[J]. Journal of Beijing Forestry University, 2017, 39(8): 98-103. DOI: 10.13332/j.1000-1522.20160265
[4]	FU Qun, XU Ming-hui, WANG Zhen-yu. Optimization of ultrasonic assisted process of extracting polyphenols from seed scales of Korean pine and their antioxidation.[J]. Journal of Beijing Forestry University, 2015, 37(11): 128-135. DOI: 10.13332/j.1000-1522.20140251
[5]	GUO Qing-qi, ZHANG Na, LIU Yi-bing, WANG Zhen-yu. Microwave and ultrasonic effects on the extraction and antioxidation of larch bark polyphenols.[J]. Journal of Beijing Forestry University, 2015, 37(1): 134-138. DOI: 10.13332/j.cnki.jbfu.2015.01.018
[6]	GUO Qing-qi, ZHANG Na, LI Meng-yun, ZHANG Zhi, WANG Zhen-yu. Relationship between polyphenols and antioxidation in larch pine cones and growing slope aspect.[J]. Journal of Beijing Forestry University, 2014, 36(1): 62-65.
[7]	ZHOU Xing-zi, WANG Zi-na, ZENG Hong-sheng, XIA Er-dong, CHEN Lin, REN Di-feng, LU Jun. Preparation process and antioxidant activity of walnut fermented drinks.[J]. Journal of Beijing Forestry University, 2013, 35(5): 139-143.
[8]	GUO Qing-qi, ZHANG Na, LI Meng-yun, ZHANG Zhi, WANG Zhen-yu. Relationship between polyphenols and antioxidation in larch pine cones and altitude[J]. Journal of Beijing Forestry University, 2013, 35(1): 59-63.
[9]	WANG Chang-lu, JIANG Shen-hua, , CHEN Zhi-qiang, CHEN Mian-hua, WANG Yu-rong, LIU Chang-jin, XIA Lian-fa. The extracting technology and antioxidant properties of the total flavonoids from the old leaves of Toona sinensis[J]. Journal of Beijing Forestry University, 2008, 30(4): 28-33.
[10]	JIN Ying, SUN Ai-dong, HU Xiao-dan, WANG Xiao-nan. Ultrasonic extraction and antioxidant activities of apple polyphenols[J]. Journal of Beijing Forestry University, 2007, 29(5): 137-141. DOI: 10.13332/j.1000-1522.2007.05.025

Cited By

Get Citation

PDF

XML

Article views (2146) PDF downloads (26)

Turn off MathJax

Article Contents

Abstract

References

Extraction of total flavonoids from Cronartium orientale aeciospores by response surface methodology and its antioxidant activity in vitro

Abstract

References

Related Articles

Catalog

Extraction of total flavonoids from Cronartium orientale aeciospores by response surface methodology and its antioxidant activity in vitro

Abstract

References

Related Articles

Catalog

Export File

Citation

Format

Content