Citation: | Ma Siyuan, Yao Jun, Li Jing, An Keyue, Zhao Rui, Zhao Nan, Zhou Xiaoyang, Chen Shaoliang. Populus euphratica PeMAX2 regulating drought tolerance in Arabidopsis thaliana[J]. Journal of Beijing Forestry University, 2024, 46(6): 106-117. DOI: 10.12171/j.1000-1522.20220494 |
MAX2 plays an important role in inhibiting plant branching and regulating strigolactone signaling pathway. MAX2 is also involved in multi-phytohormone interactions and in plant response to biotic and abiotic stresses. It has been shown that overexpression of Populus euphratica PeMAX2 can improve ionic homeostasis and the salt tolerance in transgenic plants of Arabidopsis thaliana. However, little is known about the function of PeMAX2 in drought tolerance. The objective of this study is to explore the mechanism of PeMAX2 in regulating drought tolerance of Arabidopsis thaliana.
P. euphratica PeMAX2 was overexpressed in A. thaliana, and the physiological and molecular mechanism underlying the osmotic and drought tolerance of transgenic plants was investigated in this study.
(1) The expression of PeMAX2 gene was up-regulated in P. euphratica leaves under long-term of drought stress. (2) After mannitol treatment, the seed germination rate and root length of A. thaliana overexpressing PeMAX2 were significantly higher than those of wild type and max2 mutant. The cell membrane was less damaged in PeMAX2-transgenic plants under osmotic stress compared with WT and max2. The increase of superoxide dismutase, peroxidase and catalase activities and the transcription levels of their encoding genes were higher in transgenic lines than in WT and max2 mutant under osmotic stress. As a result, the ability to regulate H2O2 was increased in root cells of transgenic lines. (3) A 10-d of soil drought decreased the chlorophyll content in all tested lines, and a more pronounced reduction was observed in WT and max2 mutant. Under drought stress, the maximum photochemical efficiency of PSⅡ, relative electron transport rate and actual quantum yield of photosynthesis were less inhibited in PeMAX2-overexpressed plants than in WT and max2. Meanwhile, transgenic plants had higher net photosynthetic rate and stomatal conductance under drought treatment, as compared with WT and mutant plants. This indicated that overexpression of PeMAX2 improved the photosynthetic capacity of transgenic plants under drought conditions. The recovery of chlorophyll content, fluorescence and photosynthesis of WT and mutant were significantly lower than that of transgenic lines after soil rehydration.
Overexpression of P. euphratica PeMAX2 improves the drought tolerance of A. thaliana. This is mainly due to the increased ability to scavenge reactive oxygen species in transgenic plants. Consequently, the oxidative damage to cell membrane and the drought inhibition of photosynthesis are alleviated in PeMAX2-transgenic plants under water stress.
[1] |
Toh S, Holbrook-Smith D, Stogios P J, et al. Structure-function analysis identifies highly sensitive strigolactone receptors in Striga[J]. Science, 2015, 350: 203−207. doi: 10.1126/science.aac9476
|
[2] |
Li B W, Gao S, Yang Z M, et al. The F-box E3 ubiquitin ligase AtSDR is involved in salt and drought stress responses in Arabidopsis[J]. Gene, 2021, 809: 146011.
|
[3] |
Carbonnel S, Torabi S, Gutjahr C. MAX2-independent transcriptional responses to rac-GR24 in Lotus japonicus roots[J]. Plant Signaling & Behavior, 2021, 16(1): 1840852.
|
[4] |
Yu H C, Wu J, Xu N F, et al. Roles of F-box proteins in plant hormone responses[J]. Acta BiochimBiophys Sin (Shanghai), 2007, 39(12): 915−922. doi: 10.1111/j.1745-7270.2007.00358.x
|
[5] |
Wang L, Wang B, Yu H, et al. Transcriptional regulation of strigolactone signalling in Arabidopsis[J]. Nature, 2020, 583: 277−281. doi: 10.1038/s41586-020-2382-x
|
[6] |
Stirnberg P, van de Sande K, Leyser H M O, et al. MAX1 and MAX2 control shoot lateral branching in Arabidopsis[J]. Development, 2002, 129(5): 1131−1141. doi: 10.1242/dev.129.5.1131
|
[7] |
Bennett T, Sieberer T, Willett B, et al. The Arabidopsis MAX pathway controls shoot branching by regulating auxin transport[J]. Current Biology, 2006, 16(6): 553−563. doi: 10.1016/j.cub.2006.01.058
|
[8] |
Hayward A, Stirnberg P, Beveridge C, et al. Interactions between auxin and strigolactone in shoot branching control[J]. Plant Physiology, 2009, 151(1): 400−412. doi: 10.1104/pp.109.137646
|
[9] |
Stirnberg P, Furner I J, Leyser H M O, et al. MAX2 participates in an SCF complex which acts locally at the node to suppress shoot branching[J]. The Plant Journal, 2007, 50(1): 80−94. doi: 10.1111/j.1365-313X.2007.03032.x
|
[10] |
王闵霞, 彭鹏, 龙海馨, 等. 独脚金内酯途径相关基因的研究进展[J]. 分子植物育种, 2014, 12(3): 603−609.
Wang M X, Peng P, Long H X, et al. Progress in cloning of strigolactone-related genes[J]. Molecular Plant Breeding, 2014, 12(3): 603−609.
|
[11] |
Waters M T, Scaffidi A, Flematti G R, et al. The origins and mechanisms of karrikin signaling[J]. Current Opinion in Plant Biology, 2013, 16(5): 667−673. doi: 10.1016/j.pbi.2013.07.005
|
[12] |
Bunsick M, Toh S, Wong C, et al. SMAX1-dependent seed germination bypasses GA signalling in Arabidopsis and Striga[J]. Nature Plants, 2020, 6(6): 646−652. doi: 10.1038/s41477-020-0653-z
|
[13] |
Bursch K, Niemann E T, Nelson D C, et al. Karrikins control seedling photomorphogenesis and anthocyanin biosynthesis through a HY5-BBX transcriptional module[J]. Plant Journal, 2021, 107(5): 1346−1362. doi: 10.1111/tpj.15383
|
[14] |
Zhao L L, Fang J J, Xing J, et al. Identification and functional analysis of two cotton orthologs of MAX2 which control shoot lateral branching[J]. Plant Molecular Biology Reporter, 2017, 35(5): 480−490. doi: 10.1007/s11105-017-1040-4
|
[15] |
Al-Babili S, Bouwmeester H J. Strigolactones, a novel carotenoid-derived plant hormone[J]. Annual Review of Plant Biology, 2015, 66(1): 161−186. doi: 10.1146/annurev-arplant-043014-114759
|
[16] |
Swarbreck S M, Guerringue Y, Matthus E, et al. Impairment in karrikin but not strigolactone sensing enhances root skewing in Arabidopsis thaliana[J]. The Plant Journal, 2019, 98(4): 607−621. doi: 10.1111/tpj.14233
|
[17] |
Osnato M. Not too short and not too long: SMAX1 optimizes hypocotyl length at warmer temperature[J]. The Plant Cell, 2022, 34(7): 2580−2581. doi: 10.1093/plcell/koac125
|
[18] |
Yao C, Finlayson S A. Abscisic acid is a general negative regulator of Arabidopsis axillary bud growth[J]. Plant Physiology, 2015, 169(1): 611−626. doi: 10.1104/pp.15.00682
|
[19] |
Li W Q, Nguyen K H, Ha C V, et al. Crosstalk between the cytokinin and MAX2 signaling pathways in growth and callus formation of Arabidopsis thaliana[J]. Biochemical and Biophysical Research Communications, 2019, 511(2): 300−306. doi: 10.1016/j.bbrc.2019.02.038
|
[20] |
Bu Q Y, Lü T X, Shen H, et al. Regulation of drought tolerance by the F-box protein MAX2 in Arabidopsis[J]. Plant Physiology, 2014, 164(1): 424−439. doi: 10.1104/pp.113.226837
|
[21] |
Li W Q, Nguyen K H, Watanabe Y, et al. OaMAX2 of Orobanche aegyptiaca and Arabidopsis AtMAX2 share conserved functions in both development and drought responses[J]. Biochemical and Biophysical Research Communications, 2016, 478(2): 521−526. doi: 10.1016/j.bbrc.2016.07.065
|
[22] |
An J P, Li R, Qu F J, et al. Apple F-box protein MdMAX2 regulates plant photomorphogenesis and stress response[J]. Frontiers in Plant Science, 2016, 7: 1685.
|
[23] |
Fu X J, Wang J, Shangguan T W, et al. SMXLs regulate seed germination under salinity and drought stress in soybean[J]. Plant Growth Regulation, 2022, 96(3): 397−408. doi: 10.1007/s10725-021-00786-6
|
[24] |
侯思源, 张会龙, 尧俊, 等. 胡杨PeREM6.5调控拟南芥水分胁迫耐受机制[J]. 北京林业大学学报, 2022, 44(9): 40−51. doi: 10.12171/j.1000-1522.20210195
Hou S Y, Zhang H L, Yao J, et al. Populus euphratica PeREM6.5 regulating tolerance mechanism to water stress in Arabidopsis thaliana[J]. Journal of Beijing Forestry University, 2022, 44(9): 40−51. doi: 10.12171/j.1000-1522.20210195
|
[25] |
武霞, 张一南, 赵楠, 等. 过表达胡杨PeAnn1负调控拟南芥的抗旱性[J]. 北京林业大学学报, 2020, 42(6): 14−25. doi: 10.12171/j.1000-1522.20200031
Wu X, Zhang Y N, Zhao N, et al. Overexpression of PeAnn1 from Populus euphratica negatively regulates drought resistance in transgenic Arabidopsis thaliana[J]. Journal of Beijing Forestry University, 2020, 42(6): 14−25. doi: 10.12171/j.1000-1522.20200031
|
[26] |
Ge X L, Zhang L, Du J J, et al. Transcriptome analysis of Populus euphratica under salt treatment and PeERF1 gene enhances salt tolerance in transgenic Populus alba × Populus glandulosa[J]. International Journal of Molecular Sciences, 2022, 23(7): 3727. doi: 10.3390/ijms23073727
|
[27] |
尧俊. 胡杨转录调节因子PeWRKY1调控离子平衡分子网络研究[D]. 北京: 北京林业大学, 2020.
Yao J. Populus euphratica transcription factor PeWRKY1 mediates signaling network conferring ionic homeostasis under salt stress[D]. Beijing: Beijing Forestry University, 2020.
|
[28] |
Abbasi G H, Ijaz M, Akhtar J, et al. Profiling of anti-oxidative enzymes and lipid peroxidation in leaves of salt tolerant and salt sensitive maize hybrids under NaCl and Cd stress[J]. Sains Malaysiana, 2016, 45(2): 177−184.
|
[29] |
Aalifar M, Aliniaeifard S, Arab M, et al. Blue light improves vase life of carnation cut flowers through its effect on the antioxidant defense system[J]. Frontiers in Plant Science, 2020, 11: 511. doi: 10.3389/fpls.2020.00511
|
[30] |
Jia M X, Jiang X R, Xu J, et al. CAT and MDH improve the germination and alleviate the oxidative stress of cryopreserved Paeonia and Magnolia pollen[J]. Acta Physiologiae Plantarum, 2018, 40(2): 1−10.
|
[31] |
Du G Y, Li X J, Wang J H, et al. Discrepancy in photosynthetic responses of the red alga Pyropia yezoensis to dehydration stresses under exposure to desiccation, high salinity, and high mannitol concentration[J]. Marine Life Science & Technology, 2021, 4(1): 10−17.
|
[32] |
Ha C V, Leyva-Gonzalez M A, Osakabe Y, et al. Positive regulatory role of strigolactone in plant responses to drought and salt stress[J]. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111(2): 851−856.
|
[33] |
Liu J W, He H Z, Vitali M, et al. Osmotic stress represses strigolactone biosynthesis in Lotus japonicus roots: exploring the interaction between strigolactones and ABA under abiotic stress[J]. Planta, 2015, 241(6): 1435−1451. doi: 10.1007/s00425-015-2266-8
|
[34] |
任广悦. 黄瓜SLs信号转导基因CsMAX2的克隆及抗逆功能验证[D]. 哈尔滨: 哈尔滨师范大学, 2020.
Ren G Y. Cloning of SLs signal transduction gene CsMAX2 in Cucumis sativus L. and verification of stress resistance[D]. Harbin: Harbin Normal University, 2020.
|
[35] |
吕天晓. 拟南芥MAX2蛋白介导ABA及抗旱反应的分子机制[D]. 长春: 中国科学院东北地理与农业生态研究所, 2015.
Lü T X. Functional analysis of MAX2 in regulating ABA signaling and drought stress response in Arabidopsis[D]. Changchun: Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 2015.
|
[36] |
Rezayian M, Niknam V, Ebrahimzadeh H. Penconazole and calcium ameliorate drought stress in canola by upregulating the antioxidative enzymes[J]. Functional Plant Biology, 2020, 47(9): 825−839. doi: 10.1071/FP19341
|