Type, distribution and quantitative characteristics of sensory organs on the larval head of <i>Tomicus brevipilosus</i> (Coleoptera: Curculionidae: Scolytinae)

Zhang Yanlin; Lu Guoyan; Xiang Yutao; Yang Bin; Li Zongbo

doi:10.12171/j.1000-1522.20230105

Journal of Beijing Forestry University > 2024 > 46(4): 63-73. > DOI: 10.12171/j.1000-1522.20230105

Zhang Yanlin, Lu Guoyan, Xiang Yutao, Yang Bin, Li Zongbo. Type, distribution and quantitative characteristics of sensory organs on the larval head of Tomicus brevipilosus (Coleoptera: Curculionidae: Scolytinae)[J]. Journal of Beijing Forestry University, 2024, 46(4): 63-73. DOI: 10.12171/j.1000-1522.20230105

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Type, distribution and quantitative characteristics of sensory organs on the larval head of Tomicus brevipilosus (Coleoptera: Curculionidae: Scolytinae)

Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, College of Biodiversity Conservation and Utilization, Southwest Forestry University, Kunming 650224, Yunnan, China

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Received Date: May 06, 2023
Revised Date: December 18, 2023
Accepted Date: December 20, 2023
Available Online: December 24, 2023

Graphical Abstract

Abstract

Abstract

Objective
To provide a scientific basis for further exploration of foraging and moving about the larval behaviors of Tomicus bravipilosus (Coleoptera: Curculionidae: Scolytinae), we investigated the morphological adaptation and function of the sensilla on the larval head of this bark beetle.
Method
We examined and compared the types, distribution, and abundance of sensory organs on the head of the first, second, and third instar larvae using scanning electron microscopy (SEM).
Result
The head shape of the larvae was hypognathous. Its head appendages contained a pair of antennae, stemmata, mandible, maxilla, maxillary palp, labial palp, as well as a single labrum and labium. Totally, there were 15 types of identified sensilla. The reduced antennae with one segment had five types of antennal sensilla, identified as sensilla basiconica type Ⅰ and sensilla twig basiconica type Ⅰ−Ⅳ. The biting mouthparts were involved in 12 types, including sensilla chaetica type Ⅰ−Ⅴ, sensilla trichodea type Ⅰ−Ⅱ, sensilla basiconica type Ⅱ, sensilla digitiformina, and sensilla twig basiconica type Ⅱ, Ⅳ, and Ⅴ. Sensilla trichodea and sensilla chaetica having smooth and nonporous surface were widespread and classified as mechanoreceptors. Sensilla basiconica and sensilla twig basiconica having the surface pores distributed exclusively on the apex of the antennae, maxillary palp and labial palp, and were classical chemoreceptors. Sensilla digitiformia having only one unit on the second segment of every maxillary palp were considered as the acoustic sensor for the mechanical vibrations. With an increase of larval instar, each sensilla types, abundance, and distribution on the head appendages kept consistent with the different instar larvae. However, sensilla sizes showed exponential growth compared with the last instar larvae.
Conclusion
This research clarifies the head structures and sensilla types, distribution, abundance, and instar changes of the sensilla on the larval head of T. brevipilosus. Chemical sensors are concentrated at the tips of the antennae, mandibular palps, and labial palps, which is a distribution pattern that aids the larvae in feeding and moving within the bark-cavities. The study has conducted a detailed exploration of the functions of the chemical sensors, which will provide a scientific basis for the next step of studying the information perception of T. brevipilosus larvae within its galleries. It is expected to take a shred of scientific evidence for further behavioral drivers of chemical sensation in the gallery of T. brevipilosus larvae.
- Tomicus brevipilosus,
- larval head,
- sensilla,
- scanning electron microscopy

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References (34)

References

[1]	彩万志, 庞雄飞, 花保祯, 等. 普通昆虫学[M]. 2版. 北京: 中国农业大学出版社, 2011: 233−235. Cai W Z, Pang X F, Hua B Z, et al. General entomology [M]. 2nd ed. Beijing: China Agriculture University Press, 2011: 233−235.
[2]	Sobral M. All traits are functional: an evolutionary viewpoint[J]. Trends in Plant Science, 2021, 26(7): 674−676. doi: 10.1016/j.tplants.2021.04.004
[3]	Alekseev M A, Sinitsina E E, Chaika S Y. Sensory organs of the antennae and mouthparts of beetle larvae (Coleoptera)[J]. Entomological Review, 2006, 86(6): 638−648. doi: 10.1134/S0013873806060042
[4]	Glendinning J I. Insect gustatory systems[M]. New York: Academic Press, 2008: 75−95.
[5]	Crespo J G. A review of chemosensation and related behavior in aquatic insects[J]. Journal of Insect Science, 2011, 11(1): 62.
[6]	Hofstetter R W, Aflitto N, Bedoya C L, et al. Vibrational behavior in bark beetles: applied aspects[M]. Cham: Springer International Publishing, 2019: 415−435.
[7]	Makarova A A, Diakova A A, Chaika S Y, et al. Scaling of the sense organs of insects(2): sensilla, discussion, conclusion[J]. Entomological Review, 2022, 102(3): 323−346. doi: 10.1134/S0013873822030058
[8]	戈峰. 害虫管理: 从“综合”到“整合”[J]. 应用昆虫学报, 2020, 57(1): 1−9. Ge F. From comprehensive to integrated pest management[J]. Chinese Journal of Applied Entomology, 2020, 57(1): 1−9.
[9]	Lieutier F, Långström B, Faccolim M. The genus Tomicus[M]. San Diego: Academic Press, 2015: 371−426.
[10]	武承旭, 臧丽鹏, 张苏芳, 等. 云南松三种同域共存切梢小蠹梢转干期的空间分布格局[J]. 生态学报, 2020, 40(11): 3646−3655. Wu C X, Zang L P, Zhang S F, et al. Spatial distribution patterns of three sympatric Tomicus species initially infesting Pinus yunnanensis trunks[J]. Acta Ecologica Sinica, 2020, 40(11): 3646−3655.
[11]	Liu F, Wu C, Zhang S, et al. Initial location preference together with aggregation pheromones regulate the attack pattern of Tomicus brevipilosus (Coleoptera: Curculionidae) on Pinus kesiya[J]. Forests, 2019, 10(2): 156. doi: 10.3390/f10020156
[12]	Borden J H, Birmingham A L, Burleigh J S. Evaluation of the push-pull tactic against the mountain pine beetle using verbenone and non-host volatiles in combination with pheromone-baited trees[J]. The Forestry Chronicle, 2006, 82(4): 579−590. doi: 10.5558/tfc82579-4
[13]	季梅, 董谢琼, 刘宏屏, 等. 云南松林纵坑切梢小蠹灾害遥感监测的初步研究[J]. 西部林业科学, 2007, 36(1): 87−90. doi: 10.3969/j.issn.1672-8246.2007.01.018 Ji M, Dong X Q, Liu H P, et al. Preliminary study on remote forest damaged sensing detection of Yunnan pine by Tomicus piniperda[J]. Journal of West China Forestry Science, 2007, 36(1): 87−90. doi: 10.3969/j.issn.1672-8246.2007.01.018
[14]	Polajnar J, Eriksson A, Lucchi A, et al. Manipulating behaviour with substrate-borne vibrations:potential for insect pest control[J]. Pest Management Science, 2015, 71(1): 15−23. doi: 10.1002/ps.3848
[15]	He J, Yan Z G, Jiang Z S. Effects of five new compounds on the larval growth and digestive physiology of the Asiatic corn borer, Ostrinia furnacalis larvae[J]. Insect Science, 2003, 10(3): 173−177. doi: 10.1111/j.1744-7917.2003.tb00381.x
[16]	张梦蝶, 钱路兵, 泽桑梓, 等. 云南切梢小蠹幼虫表皮碳氢化合物与龄数的相关性[J]. 林业科学, 2021, 57(5): 151−159. Zhang M D, Qian L B, Ze S Z, et al. Correlation between cuticular hydrocarbons and instar numbers of the larvae of Yunnan shoot borer, Tomicus yunnanensis (Coleoptera: Scolytidae)[J]. Scientia Silvae Sinicae, 2021, 57(5): 151−159.
[17]	Zacharuk R Y. Antennae and sensilla[M]. Oxford:Pergamon, 1985.
[18]	Yang Y, Ren L, Xu L, et al. Comparative morphology of sensilla on the antennae, maxillary and labial palps in different larval instars of Cryptorrhynchus lapathi (Linnaeus) (Coleoptera: Curculionidae)[J]. Zoologischer Anzeiger, 2019, 283: 93−101. doi: 10.1016/j.jcz.2019.09.003
[19]	Shi X, Shen J C, Zhang S F, et al. Comparative analysis of the type and number of larval sensilla on the antennae and mouthparts of Ips typographus and Ips subelongatus using SEM[J]. Zoologischer Anzeiger, 2020, 289: 18−25. doi: 10.1016/j.jcz.2020.08.007
[20]	徐丽丽. 沟胫天牛亚科七种天牛不同虫态触角、下颚须和下唇须的感器研究 [D]. 北京: 北京林业大学, 2016. Xu L L. Sensilla on antenna, maxillary palp and labial palps of seven Lamiinae longhorned beetle species at different life stages[D]. Beijing: Beijing Forestry University, 2016.
[21]	Speirs R D, White G D, Wilson J L. SEM observations of rice weevil larvae, Sitophilus oryzae (L.) (Coleoptera: Curculionidae)[J]. Journal of the Kansas Entomological Society, 1986, 59(2): 390−394.
[22]	Xu L, Zhang L, Yang Y, et al. Morphology of antennal, maxillary palp and labial palp sensilla in different larval instars of the Asian long-horned beetle, Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae)[J]. Acta Zoologica, 2017, 98(1): 20−31. doi: 10.1111/azo.12146
[23]	Ryan M F, Behan M. The sensory receptors of Tribolium larvae[J]. Physiological Zoology, 1973, 46(3): 238−244. doi: 10.1086/physzool.46.3.30155605
[24]	Giglio A, Ferrero E A, Perrotta E, et al. Ultrastructure and comparative morphology of mouth-part sensilla in ground beetle larvae (Insecta, Coleoptera, Carabidae)[J]. Zoologischer Anzeiger, 2003, 242(3): 277−292. doi: 10.1078/0044-5231-00104
[25]	韩潇, 段彦丽, 李秀芬, 等. 花绒寄甲1龄幼虫触角及口器感器的超微结构[J]. 林业科学研究, 2021, 34(2): 180−184. doi: 10.13275/j.cnki.lykxyj.2021.02.020 Han X, Duan Y L, Li X F, et al. Sensilla ultrastructure of antennae and mouthparts of the first instar larvae of Dastarcus helophoroides (Coleoptera: Bothrideridae)[J]. Forest Research, 2021, 34(2): 180−184. doi: 10.13275/j.cnki.lykxyj.2021.02.020
[26]	Eilers E J, Talarico G, Hansson B S, et al. Sensing the underground – ultrastructure and function of sensory organs in root-feeding Melolontha melolontha (Coleoptera: Scarabaeinae) larvae[J]. PLoS One, 2012, 7(7): e41357. doi: 10.1371/journal.pone.0041357
[27]	Mckenna D D, Shin S, Aheens D, et al. The evolution and genomic basis of beetle diversity[J]. Proceedings of the National Academy of Sciences, 2019, 116(49): 24729−24737. doi: 10.1073/pnas.1909655116
[28]	Keil T A. Functional morphology of insect mechanoreceptors[J]. Microscopy Research and Technique, 1997, 39(6): 506−531. doi: 10.1002/(SICI)1097-0029(19971215)39:6<506::AID-JEMT5>3.0.CO;2-B
[29]	Isidoro N, Romani R, Bin F. Antennal multiporous sensilla: Their gustatory features for host recognition in female parasitic wasps (Insecta, Hymenoptera: Platygastroidea)[J]. Microscopy Research and Technique, 2001, 55(5): 350−358. doi: 10.1002/jemt.1183
[30]	Beutel R G, Yavorskaya M. Structure and evolution of mouthparts in Coleoptera[M]. Cham: Springer International Publishing, 2019: 387−418.
[31]	Basibuyuk H H, Quicke D L J. Gross morphology of multiporous plate sensilla in the Hymenoptera (Insecta)[J]. Zoologica Scripta, 1999, 28(1−2): 51−67. doi: 10.1046/j.1463-6409.1999.00007.x
[32]	Keil T A. Sensilla on the maxillary palps of Helicoverpa armigera caterpillars: in search of the CO₂-receptor[J]. Tissue and Cell, 1996, 28(6): 703−717. doi: 10.1016/S0040-8166(96)80073-5
[33]	Zacharuk R Y, Albert P J, Bellamy F W. Ultrastructure and function of digitiform sensilla on the labial palp of a larval elaterid (Coleoptera)[J]. Canadian Journal of Zoology, 1977, 55(3): 569−578. doi: 10.1139/z77-072
[34]	Broad G R, Quicke D L J. The adaptive significance of host location by vibrational sounding in parasitoid wasps[J]. Proceedings of the Royal Society of London Series B: Biological Sciences, 2000, 267: 2403−2409. doi: 10.1098/rspb.2000.1298

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Type, distribution and quantitative characteristics of sensory organs on the larval head of Tomicus brevipilosus (Coleoptera: Curculionidae: Scolytinae)