Root development and genetic regulation in <i>Populus euphratica</i> under salt stress

Zhang Bo; Lu Kaiyan; Zhang Xiaoyu; Wu Rongling

doi:10.12171/j.1000-1522.20230374

Journal of Beijing Forestry University > 2025 > 47(1): 72-84. > DOI: 10.12171/j.1000-1522.20230374

Zhang Bo, Lu Kaiyan, Zhang Xiaoyu, Wu Rongling. Root development and genetic regulation in Populus euphratica under salt stress[J]. Journal of Beijing Forestry University, 2025, 47(1): 72-84. DOI: 10.12171/j.1000-1522.20230374

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Root development and genetic regulation in Populus euphratica under salt stress

1.
School of Science, Beijing Forestry University, Beijing 100083, China
2.
Center of Computational Biology, School of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China

More Information

Received Date: December 28, 2023
Revised Date: June 05, 2024
Accepted Date: December 21, 2024
Available Online: December 26, 2024

Graphical Abstract

Abstract

Abstract

Objective
This paper studies the growth adaptability pattern of Populus euphratica root under normal and salt stress conditions, effectively locates the significant quantitative trait loci (QTL) affecting the phenotypic traits of P. euphratica root, visualizes genetic regulation network, and explores the genetic regulation mechanism of phenotypic traits of P. euphratica root under salt stress.
Method
Based on phenotypic and genotypic data of P. euphratica under normal and salt stress conditions, statistical methods such as principal component analysis, variance analysis, and dynamic model fitting were used to analyze phenotypic variation patterns, locate significant QTL by functional mapping, and visualize the genetic network of phenotypic traits in root system under salt stress.
Result
(1) Correlation analysis showed that P. euphratica root traits exhibited high synergy under normal conditions, but under salt stress, the roots reduced in number while increased in average length. PCA identified main root length, main root surface area, and main root number as the primary phenotypic traits for study. Compared with normal conditions, salt stress inhibited the growth of these three traits. Comparison of goodness-of-fit R² revealed that the Gompertz model performed best among Logistic, Richards, and Weibull growth equations. (2) Under normal conditions, 100, 89, and 85 significant QTLs regulating main root length, main root surface area, and main root number were identified, respectively, mainly distributed on linkage groups 1, 4, 5, 14, and 15. Under salt stress, 91, 85, and 87 significant QTLs regulating these three traits were located, concentrated on linkage groups 2, 3, 5, 13, and 18. (3) Genetic effect analysis showed that the significant QTLs affecting main root length exhibited continuously increasing genetic effects over time under both conditions. The significant QTLs influencing main root number mainly showed a continuous increase, with some loci displaying trends of first increasing then decreasing or first decreasing then increasing. The significant QTLs affecting main root surface area exhibited three patterns under normal conditions: first increasing then decreasing, continuously fluctuating upwards, and first increasing then decreasing before increasing again, while most showed a continuous increase under salt stress. (4) Under normal conditions, 21, 14, and 14 candidate genes were annotated for traits of main root length, main root surface area, and main root number, respectively, mainly involved in basic metabolic processes, auxin transport, and chromosome segregation functions. Under salt stress, 19, 17, and 15 candidate genes were annotated for these traits, respectively, primarily enriched in stress response, redox balance, iron ion transport, and tRNA modification functions. (5) The genetic network was visualized, revealing hub genes (LOC105114908 and LOC105120566) might involve in P. euphratica’s response to salt stress.
Conclusion
Salt stress significantly affects the growth and development of P. euphratica roots. Using various statistical models, significant QTLs influencing root growth are located, demonstrating their dynamic genetic effects with environmental changes. The results provide new insights into understanding the genetic regulatory basis of P. euphratica’s adaptation to salt stress and offer methodological support for forest tree genetic improvement research.
- Populus euphratica,
- salt stress,
- functional mapping,
- genetic effects,
- genetic network,
- quantitative trait loci (QTL),
- taproot trait

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References (31)

References

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Root development and genetic regulation in Populus euphratica under salt stress