Analysis of different expressiongenes between cross-compatibility and cross-incompatibility within pistils of <i>Lilium</i> spp

WANG Jie; JIA Yue-hui; ZHANG Ke-zhong; CUI Jin-teng

doi:10.13332/j.1000-1522.20160292

Journal of Beijing Forestry University > 2017 > 39(2): 82-91. > DOI: 10.13332/j.1000-1522.20160292

WANG Jie, JIA Yue-hui, ZHANG Ke-zhong, CUI Jin-teng. Analysis of different expressiongenes between cross-compatibility and cross-incompatibility within pistils of Lilium spp[J]. Journal of Beijing Forestry University, 2017, 39(2): 82-91. DOI: 10.13332/j.1000-1522.20160292

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Analysis of different expressiongenes between cross-compatibility and cross-incompatibility within pistils of Lilium spp

1.
Landscape Architecture College, Beijing University of Agriculture, Beijing, 102206, P.R.China
2.
Beijing Laboratory of Urban and Rural Ecological Environment, Beijing, 102206, P.R.China

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Received Date: September 13, 2016
Revised Date: November 22, 2016
Published Date: January 31, 2017

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Abstract

Abstract

The hybridization of Lilium Oriental hybrids and wild lily of Sect. Sinomartagon Comber is the farmost genetic relationship and it's difficult to achieve. Researching the different expression genes in pistil of cross-compatibility and cross-incompatibility may explain the mechanism of causing orientated growth of pollen tube and the molecular mechanism of incompatible hybridization, and provide the basis for overcoming cross-incompatibility. We constructed a forward suppression subtractive hybridization (SSH) library by using the cross-incompatible Lilium Oriental hybrid pistils as the tester, and the cross-compatible ones as the driver. 180 positive clones were randomly screened out. Through sequencing, deleting the bad, removing the redundant and aligning the sequences, 113 ESTs were found to be homologous with known sequences in the bioinformatics databases. RT-PCR was performed to test the 10 selected different expression genes, which showed 1 gene was up-regulated, 7 genes were down-regulated and 2 genes were almost unchanged. Gene annotation and functional category analysis showed that the different expression genes mainly contained signal transduction(including PP2A-3, CDPK and small GTP-binding protein), transport-related(including clpB and CAT2), stress/defense(including ATPase and pyrophosphatase proton pump), protein fate related genes(including 60S ribosomal protein). It was speculated that the incompatibility in the section cross of Oriental hybrids ×Lilium pumilum was probably resulted from the abnormal signal communication between pistils and pollens, the deduced substance transport ability in pistils, the weaker stress resistance ability in pistils after incompatible hybridization.
- Lilium,
- cross-incompatiblity,
- suppression subtractive hybridization,
- differential expression genes in pistil

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References

[1]	VAN TUYL J M, VAN DIJKEN A, CHI H S, et al. Breakthroughs in interspecific hybridization of lily[J]. Acta Hort, 2000, 508:83-88. https://www.researchgate.net/publication/40147280_Breakthroughs_in_interspecific_hybridization_of_lily
[2]	HIGASHIYAMA T.Peptide signaling in pollen-pistil interactions[J].Plant Cell Physiol, 2010, 51(2): 177-189. doi: 10.1093/pcp/pcq008
[3]	MÁRTON M L, FASTNER A, UEBLER S, et al. Overcoming hybridization barriers by the secretion of the maize pollen tube attractant ZmEA1 from Arabidopsis ovules[J]. Current Biology, 2012, 22(13): 1194-1198. doi: 10.1016/j.cub.2012.04.061
[4]	OKUDA S, TSUTSUI H, SHIINA K, et al. Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells[J]. Nature, 2009, 458(7236): 357-361. doi: 10.1038/nature07882
[5]	KANAOKA M M, KAWANO N, MATSUBARA Y, et al. Identification and characterization of TcCRP1, a pollen tube attractant from Torenia concolor[J]. Annals of Botany, 2011, 108(4): 739-747. doi: 10.1093/aob/mcr111
[6]	WU H, DE GRAAF B, MARIANI C, et al. Hydroxyproline-rich glycoproteins in plant reproductive tissues: structure, functions and regulation[J]. Cellular and Molecular Life Sciences CMLS, 2001, 58(10): 1418-1429. doi: 10.1007/PL00000785
[7]	HISCOCK S J, DEWEY F M, COLEMAN J O D, et al. Identification and localization of an active cutinase in the pollen of Brassica napus L.[J]. Planta, 1994, 193(3): 377-384. doi: 10.1007/BF00201816
[8]	BOSCH M, CHEUNG A Y, HEPLER P K. Pectin methylesterase, a regulator of pollen tube growth[J]. Plant Physiology, 2005, 138(3): 1334-1346. doi: 10.1104/pp.105.059865
[9]	BOSCH M, HEPLER P K. Silencing of the tobacco pollen pectin methylesterase NtPPME1 results in retarded in vivo pollen tube growth[J]. Planta, 2006, 223(4): 736-745. doi: 10.1007/s00425-005-0131-x
[10]	ZHANG Y, MCCORMICK S. The regulation of vesicle trafficking by small GTPases and phospholipids during pollen tube growth[J]. Sexual plant reproduction, 2010, 23(2): 87-93. doi: 10.1007/s00497-009-0118-z
[11]	ZOU Y, AGGARWAL M, ZHENG W G, et al. Receptor-like kinases as surface regulators for RAC/ROP-mediated pollen tube growth and interaction with the pistil[J]. AoB Plants, 2011, 2011: plr017. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=PubMed000002185057
[12]	PALANIVELU R, BRASS L, EDLUND A F, et al. Pollen tube growth and guidance is regulated by POP2, an Arabidopsis gene that controls GABA levels[J]. Cell, 2003, 114(1): 47-59. doi: 10.1016/S0092-8674(03)00479-3
[13]	LANTIN S, MARTIN O B, MATTON D P. Pollination, wounding and jasmonate treatments induce the expression of a developmentally regulated pistil dioxygenase at a distance, in the ovary, in the wild potato Solanum chacoense Bitt[J]. Plant Molecular Biology, 1999, 41(3): 371-386. doi: 10.1023/A:1006375522626
[14]	GOLDMAN M H, GOLDBERG R B, MARIANI C. Female sterile tobacco plants are produced by stigma-specific cell ablation[J]. The EMBO Journal, 1994, 13(13): 2976. doi: 10.1002/j.1460-2075.1994.tb06596.x
[15]	QUIAPIM A C, BRITO M S, BERNARDES L A S, et al. Analysis of the Nicotiana tabacum stigma/style transcriptome reveals gene expression differences between wet and dry stigma species[J]. Plant Physiology, 2009, 149(3): 1211-1230. doi: 10.1104/pp.108.131573
[16]	HAMMOND-KOSACK K E, JONES J D. Resistance gene-dependent plant defense responses[J]. The Plant Cell, 1996, 8(10): 1773. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=4e36b1e4eb51ab0b9e5543a0a2317932
[17]	MCINNIS S M, EMERY D C, PORTER R, et al. The role of stigma peroxidases in flowering plants: insights from further characterization of a stigma-specific peroxidase (SSP) from Senecio squalidus (Asteraceae)[J]. Journal of Experimental Botany, 2006, 57(8): 1835-1846. doi: 10.1093/jxb/erj182
[18]	SEPÚLVEDA-JIMÉNEZ G, RUEDA-BENÍTEZ P, PORTA H, et al. A red beet (Beta vulgaris) UDP-glucosyltransferase gene induced by wounding, bacterial infiltration and oxidative stress[J]. Journal of Experimental Botany, 2005, 56(412): 605-611. doi: 10.1093/jxb/eri036
[19]	CHEUNG A Y, WANG H, WU H. A floral transmitting tissue-specific glycoprotein attracts pollen tubes and stimulates their growth[J]. Cell, 1995, 82(3): 383-393. doi: 10.1016/0092-8674(95)90427-1
[20]	MULTANI D S, BRIGGS S P, CHAMBERLIN M A, et al. Loss of an MDR transporter in compact stalks of maize br2 and sorghum dw3 mutants[J]. Science, 2003, 302(5642): 81-84. doi: 10.1126/science.1086072
[21]	OTSU C T, DE MOLFETTA J B, DA SILVA L R, et al. NtWBC1, an ABC transporter gene specifically expressed in tobacco reproductive organs[J]. Journal of Experimental Botany, 2004, 55(403): 1643-1654. doi: 10.1093/jxb/erh195
[22]	ALONI R, ALONI E, LANGHANS M, et al. Role of auxin in regulating Arabidopsis flower development[J]. Planta, 2006, 223(2): 315-328. doi: 10.1007/s00425-005-0088-9
[23]	FRY S C, MCDOUGALL G J, LORENCES E P, et al. Oligosaccharins from xyloglucan and cellulose: modulators of the action of auxin and H⁺ on plant growth[J]. Symposia of the Society for Experimental Biology, 1989, 44: 285-298. https://www.ncbi.nlm.nih.gov/pubmed/2130516/
[24]	MÓL R, FILEK M, MACHACKOVA I, et al. Ethylene synthesis and auxin augmentation in pistil tissues are important for egg cell differentiation after pollination in maize[J]. Plant and Cell Physiology, 2004, 45(10): 1396-1405. doi: 10.1093/pcp/pch167
[25]	赵鹏飞.烟草花粉管内吞作用机制的细胞学和蛋白质组学研究[D].郑州: 河南农业大学, 2011. http://cdmd.cnki.com.cn/Article/CDMD-10466-1012275295.htm ZHAO P F. Cellular and proteomic analysis endocytosis mechanism involved in pollen-tube growth in Nicotiana tabacum[D]. Zhengzhou: Henan Agricultural University, 2011. http://cdmd.cnki.com.cn/Article/CDMD-10466-1012275295.htm
[26]	刘珠琴.罂粟科植物自交不亲和反应中信号转导的研究进展[J].生命科学研究, 2010, 14(2): 172-176. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=smkxyj201002015 LIU Z Q. Advances in signal transduction during self-incompatibility response of papaveraceae[J]. Life Science Research, 2010, 14(2): 172-176. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=smkxyj201002015
[27]	MALHÓ R. The pollen tube: a model system for cell and molecular biology studies[M]//MALHÓ R. The pollen tube. Berlin: Springer, 2006: 1-13. https://www.researchgate.net/publication/225573209_The_Pollen_Tube_A_Model_System_for_Cell_and_Molecular_Biology_Studies
[28]	FERNANDO D D. Characterization of pollen tube development in Pinus strobus (Eastern white pine) through proteomic analysis of differentially expressed proteins[J]. Proteomics, 2005, 5(18): 4917-4926. doi: 10.1002/pmic.200500009
[29]	TADEGE M, KUHLEMEIER C. Aerobic fermentation during tobacco pollen development[J]. Plant Molecular Biology, 1997, 35(3): 343-354. doi: 10.1023/A:1005837112653
[30]	KACHROO A, NASRALLAH M E, NASRALLAH J B. Self-incompatibility in the Brassicaceae receptor-ligand signaling and cell-to-cell communication[J]. The Plant Cell, 2002, 14(Suppl.1): S227-S238. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_JJ0211958797
[31]	TAKAYAMA S, SHIMOSATO H, SHIBA H, et al. Direct ligand-receptor complex interaction controls brassica self-incompatibility[J]. Nature, 2001, 413(6855): 534-538. doi: 10.1038/35097104
[32]	SWANSON R, CLARK T, PREUSS D. Expression profiling of Arabidopsis stigma tissue identifies stigma-specific genes[J]. Sexual Plant Reproduction, 2005, 18(4): 163-171. doi: 10.1007/s00497-005-0009-x
[33]	D'AGOSTINO N, PIZZICHINI D, CHIUSANO M L, et al. An EST database from saffron stigmas[J]. BMC Plant Biology, 2007, 7(1): 35. doi: 10.1186/1471-2229-7-35
[34]	LI M, XU W, YANG W, et al. Genome-wide gene expression profiling reveals conserved and novel molecular functions of the stigma in rice[J]. Plant Physiology, 2007, 144(4): 1797-1812. doi: 10.1104/pp.107.101600
[35]	ALLEN A M, LEXER C, HISCOCK S J. Comparative analysis of pistil transcriptomes reveals conserved and novel genes expressed in dry, wet, and semidry stigmas[J]. Plant Physiology, 2010, 154(3): 1347-1360. doi: 10.1104/pp.110.162172
[36]	QUIAPIM A C, BRITO M S, BERNARDES L A S, et al. Analysis of the Nicotiana tabacum stigma/style transcriptome reveals gene expression differences between wet and dry stigma species[J]. Plant Physiology, 2009, 149(3): 1211-1230. doi: 10.1104/pp.108.131573
[37]	JOHNSON M A, PREUSS D. On your mark, get set, GROW! LePRK2-LAT52 interactions regulate pollen tube growth[J]. Trends in Plant Science, 2003, 8(3): 97-99. doi: 10.1016/S1360-1385(03)00009-8
[38]	STONE S L, ANDERSON E M, MULLEN R T, et al. ARC1 is an E3 ubiquitin ligase and promotes the ubiquitination of proteins during the rejection of self-incompatible brassica pollen[J]. The Plant Cell, 2003, 15(4): 885-898. doi: 10.1105/tpc.009845
[39]	POLLAK P E, HANSEN K, ASTWOOD J D, et al. Conditional male fertility in maize[J]. Sexual Plant Reproduction, 1995, 8(4): 231-241. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=HighWire000002227103
[40]	TEGEDER M, RENTSCH D. Uptake and partitioning of amino acids and peptides[J]. Molecular Plant, 2010, 3(6): 997-1011. doi: 10.1093/mp/ssq047
[41]	李素娟.细胞质膜质子泵在拟南芥花粉萌发过程中的作用[D].石家庄: 河北师范大学, 2007. http://cdmd.cnki.com.cn/Article/CDMD-10094-2007151459.htm LI S J. Plasma membrance H⁺-ATPase is involved in pollen genmination regulation in Arabidopsis thaliana[D].Shijiazhuang: Hebei Normal University, 2007. http://cdmd.cnki.com.cn/Article/CDMD-10094-2007151459.htm
[42]	LOVY-WHEELER A, KUNKEL J G, ALLWOOD E G, et al. Oscillatory increases in alkalinity anticipate growth and may regulate actin dynamics in pollen tubes of lily[J]. The Plant Cell, 2006, 18(9): 2182-2193. doi: 10.1105/tpc.106.044867
[43]	USHIJIMA K, SASSA H, DANDEKAR A M, et al. Structural and transcriptional analysis of the self-incompatibility locus of almond: identification of a pollen-expressed F-box gene with haplotype-specific polymorphism[J]. The Plant Cell, 2003, 15(3): 771-781. doi: 10.1105/tpc.009290
[44]	XU X H, CHEN H, SANG Y L, et al. Identification of genes specifically or preferentially expressed in maize silk reveals similarity and diversity in transcript abundance of different dry stigmas[J]. BMC Genomics, 2012, 13(1): 1. doi: 10.1186/1471-2164-13-1
[45]	CHAE K, ZHANG K, ZHANG L, et al. Two SCA (stigma/style cysteine-rich adhesin) isoforms show structural differences that correlate with their levels of in vitro pollen tube adhesion activity[J]. Journal of Biological Chemistry, 2007, 282(46): 33845-33858. doi: 10.1074/jbc.M703997200
[46]	KONG L, WANG M, WANG Q, et al. Protein phosphatases 1 and 2A and the regulation of calcium uptake and pollen tube development in Picea wilsonii[J]. Tree Physiology, 2006, 26(8): 1001-1012. doi: 10.1093/treephys/26.8.1001
[47]	KIM Y E, HIPP M S, BRACHER A, et al. Molecular chaperone functions in protein folding and proteostasis[J]. Annual Review of Biochemistry, 2013, 82: 323-355. doi: 10.1146/annurev-biochem-060208-092442
[48]	张媛华, 张韶杰, 李瑾, 等.胞外ATP对泡桐花粉萌发和花粉管伸长的效应及其与H₂O₂的关系[J].安徽农业科学, 2011, 39(5): 2572-2573, 2598. doi: 10.3969/j.issn.0517-6611.2011.05.003 ZHANG Y H, ZHANG S J, LI J, et al. The effect sofe ATP and its relationship with H₂O₂ in pollen germination and tube growth of P.tomentosa steud[J].Journal of Anhui Agri Sci, 2011, 39(5):2572-2573, 2598. doi: 10.3969/j.issn.0517-6611.2011.05.003
[49]	QUIAPIM A C, BRITO M S, BERNARDES L A S, et al. Analysis of the Nicotiana tabacum stigma/style transcriptome reveals gene expression differences between wet and dry stigma species[J]. Plant Physiology, 2009, 149(3): 1211-1230. doi: 10.1104/pp.108.131573
[50]	孙艳香, 冯雪, 贾永红, 等.植物"液泡膜" H⁺-PPase的功能与应用[J].云南农业大学学报:自然科学版, 2014, 29 (4): 591-596. http://www.cnki.com.cn/Article/CJFDTotal-YNDX201404025.htm SUN Y X, FENG X, JIA Y H, et al. Function and application of "Tonoplast" H⁺ -PPase from plant[J]. Journal of Yunnan Agricultural University:Natural Science, 2014, 29(4): 591-596. http://www.cnki.com.cn/Article/CJFDTotal-YNDX201404025.htm
[51]	DE GRAAF B H J, RUDD J J, WHEELER M J, et al. Self-incompatibility in Papaver targets soluble inorganic pyrophosphatases in pollen[J]. Nature, 2006, 444(7118): 490-493. doi: 10.1038/nature05311
[52]	FENG H, CHEN Q, FENG J, et al. Functional characterization of the Arabidopsis eukaryotic translation initiation factor 5A-2 that plays a crucial role in plant growth and development by regulating cell division, cell growth, and cell death[J]. Plant Physiology, 2007, 144(3): 1531-1545. doi: 10.1104/pp.107.098079

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Analysis of different expressiongenes between cross-compatibility and cross-incompatibility within pistils of Lilium spp