Origin identification of 2n female gamete of <i>Populus tomentosa</i> triploid hybrids

MIN Yu; KANG Ning; SUO Yu-jing; TIAN Ju; KANG Xiang-yang

doi:10.13332/j.1000-1522.20170001

Journal of Beijing Forestry University > 2017 > 39(5): 17-24. > DOI: 10.13332/j.1000-1522.20170001

MIN Yu, KANG Ning, SUO Yu-jing, TIAN Ju, KANG Xiang-yang. Origin identification of 2n female gamete of Populus tomentosa triploid hybrids[J]. Journal of Beijing Forestry University, 2017, 39(5): 17-24. DOI: 10.13332/j.1000-1522.20170001

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Origin identification of 2n female gamete of Populus tomentosa triploid hybrids

MIN Yu^1,2,,
KANG Ning^1,2,
SUO Yu-jing^1,2,
TIAN Ju³,
KANG Xiang-yang^1,2, ,

1.
College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083, P. R. China
2.
Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing, 100083, P. R. China
3.
Inner Mongolia Hesheng Institute of Ecological Sciences & Technology, Hohhot, Inner Mongolia, 011500, P. R. China

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Received Date: December 27, 2016
Revised Date: March 02, 2017
Published Date: April 30, 2017

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Abstract

Abstract

Due to the asynchronism during the process of megaspore mother cell meiosis, triploid poplars which were induced by chromosome doubling of megaspores through physical or chemical treatment could be either derived from the first-division restitution (FDR) or the second-division restitution (SDR). And it is difficult to identify the origin of the obtained triploids through their phenotypes. However, molecular marker analysis provides an efficient way to identify the origin of 2n female gametes. In this study, 87 triploid Populus tomentosa hybrids were induced by chromosome doubling of megaspores through high temperature treatment. Using these 87 triploids and their parents as material, five appropriate primers (female parent has heterozygous alleles which are different with alleles of male parent) with low recombination rate were selected to avoid the impact of homologous recombination on identifying the origin of 2n gametes. Based on these five SSR primers, results were shown that relative allele configurations at SSR loci of 2n female gametes in different triploids were inconsistent. Using the selected five SSR markers with low recombination rate, we identified the origin of 2n gametes of P. tomentosa triploid hybrids. It was shown that 35 triploids were originated from FDR 2n gametes, and the other 52 triploids were originated from SDR 2n gametes. Otherwise, five SSR primers with high recombination frequencies were detected and analyzed. The results confirmed that the randomly selected SSR primers used for the identification of origin were not completely reliable, only the SSR loci with low recombination frequencies can be used to identify the origin of 2n gametes accurately. The identification study of the origin of 2n gametes of triploid P. tomentosa is of great importance for the heterozygosity analysis of FDR and SDR 2n gametes, as well as the breeding strategy establishment for triploid P. tomentosa.
- Popolus tomentosa,
- 2n female gamete,
- chromosome doubling,
- FDR,
- SDR

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References (36)

References

[1]	EINSPAHR D W, YCKOFF G W W. Aspen hybrid promise future source of Lake states fiber[J].Pulp and Paper, 1975, 49(12): 118-119.
[2]	朱之悌, 林惠斌, 康向阳.毛白杨异源三倍体B301等无性系选育的研究[J].林业科学, 1995, 31(6):499-505. http://www.cnki.com.cn/Article/CJFDTotal-LYKE506.003.htm ZHU Z T, LIN H B, KANG X Y.Studies on allotriploid breeding of Populus tomentosa B301 clones[J].Scientia Silvae Sinicae, 1995, 31(6): 499-505. http://www.cnki.com.cn/Article/CJFDTotal-LYKE506.003.htm
[3]	张守攻, 陈成彬, 韩素英, 等.中国部分杨属植物的染色体数目[J].植物分类学报, 2005, 43(6):539-544. http://d.old.wanfangdata.com.cn/Periodical/zwflxb200506007 ZHANG S G, CHEN C B, HAN S Y, et al. Chromosome numbers of some Populus taxa from China[J]. Journal of Systematics and Evolution, 2005, 43(6):539-544. http://d.old.wanfangdata.com.cn/Periodical/zwflxb200506007
[4]	陈成彬, 齐力旺, 张守攻, 等.三倍体杨树核型分析[J].武汉植物学研究, 2004, 22(6):565-567. doi: 10.3969/j.issn.2095-0837.2004.06.017 CHEN C B, QI L W, ZHANG S G, et al. The karyptype analysis of triploid poplar[J]. Journal of Wuhan Botanical Research, 2004, 22(6):565-567. doi: 10.3969/j.issn.2095-0837.2004.06.017
[5]	康向阳, 王君.杨树多倍体诱导技术研究[M].北京:科学出版社, 2010. KANG X Y, WANG J.Poplar polyploid induction technologyresearch[M].Beijing: Science Press, 2010.
[6]	黄东森, 朱湘渝, 王瑞玲, 等.中林46等12个杨树新品种杂交育种[C]//涂忠虞, 黄敏仁.阔叶树遗传改良.北京: 科学技术文献出版社, 1991: 1-19. HUANG D S, ZHU X Y, WANG R L, et al.Crossbreeding of 12 new poplar varieties including zhonglin 46[C]//TU Z Y, HUANG M R. Broad-leaved tree genetic improvement.Beijing: Science Technology Literature Press, 1991: 1-19.
[7]	康向阳.关于杨树多倍体育种的几点认识[J].北京林业大学学报, 2010, 32(5):149-153. http://j.bjfu.edu.cn/article/id/9474 KANG X Y. Some understandings on polyploid breeding of poplars[J].Journal of Beijing Forestry University, 2010, 32(5): 149-153. http://j.bjfu.edu.cn/article/id/9474
[8]	WINTON L, EINSPAHR D.Tetraploid aspen production using unreduced triploid pollen[J]. Forest Science, 1970, 16(2): 180-182. https://www.researchgate.net/publication/45162918_Tetraploid_Aspen_production_using_unreduced_triploid_pollen
[9]	HARDER M, VERHAGEN S, WINTON L, et al. Tetraploid aspen production using unreduced pollen from triploid males[J]. Forest Science, 1976, 22(3):329-330. https://www.researchgate.net/publication/44883608_Tetraploid_Aspen_production_using_unreduced_pollen_from_triploid_males
[10]	MENDIBURU A O, PELOQUIN S J.The significance of 2n gametes in potato breeding[J]. Theoretical and Applied Genetics, 1977, 49(2):53-61. doi: 10.1007/BF00275164
[11]	张正海, 康向阳.植物2n配子发生及其遗传标记研究进展[J].遗传, 2006, 28(1):105-109. http://d.old.wanfangdata.com.cn/Periodical/yc200601019 ZHANG Z H, KANG X Y. Advances in researches on genetic markers of 2n gametes[J]. Hereditas (Beijing), 2006, 28(1):105-109. http://d.old.wanfangdata.com.cn/Periodical/yc200601019
[12]	CARPUTO D, FRUSCIANTE L, PELOQUIN S J.The role of 2n gametes and endosperm balance number in the origin and evolution of polyploids in the tuber-bearing Solanums[J]. Genetics, 2003, 163(1):287-294. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=PubMed000001252910
[13]	HERMSEN J G T.Mechanisms and genetic implications of 2n gamete formation[J].Iowa State Journal of Research, 1984, 58(4):421-434. https://www.researchgate.net/publication/40162285_Hermsen_JGTh_1984_b_Mechanisms_and_genetic_implications_of_2n_gamete_formation_Iowa_State_J_Res
[14]	PELOQUIN S J, BOITEUX L S, SIMON P W, et al.A chromosome specific estimate of transmission of heterozygosity by 2n gametes in potato[J]. Journal of Heredity, 2008, 99(2):177-181. doi: 10.1093/jhered/esm110
[15]	DONG C B, MAO J F, SUO Y J, et al.A strategy for characterization of persistent heteroduplex DNA in higher plants[J]. The Plant Journal, 2014, 80(2):282-291. doi: 10.1111/tpj.12631
[16]	CICATELLI A, LUPI R, SALA F, et al.Genetic biodiversity and phylogenetic studies in poplar by means of the metallothionein multigene mamily[M]. New York: In Tech, 2012:113-114.
[17]	PAKULL B, GROPPE K, MECUCCI F, et al.Genetic mapping of linkage group XIX and identification of sex-linked SSR markers in a Populus tremula×Populus tremuloides cross[J]. Canadian Journal of Forest Research, 2011, 41(2):245-253. doi: 10.1139/X10-206
[18]	FERRANTE S P, LUCRETTI S, REALE S, et al. Assessment of the origin of new citrus tetraploid hybrids(2n=4x) by means of SSR markers and PCR based dosage effects[J].Euphytica, 2009, 173(2): 223-233.
[19]	CHEN C L, YON M T, O'MALLEY D, et al. Origin and frequency of 2n gametes in Citrus sinensis×Poncirus trifoliata and their reciprocal crosses[J]. Plant Science, 2008, 174(1): 1-8. doi: 10.1016/j.plantsci.2007.08.005
[20]	PAQUES F, HABER J E.Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae[J]. Microbiology & Molecular Biology Reviews Mmbr, 1999, 63(2):349-404. https://www.ncbi.nlm.nih.gov/pubmed/10357855
[21]	MCMAHILL M S, SHAM C W, BISHOP D K.Synthesis-dependent strand annealing in meiosis[J].Plos Biology, 2007, 5(11):e299. doi: 10.1371/journal.pbio.0050299
[22]	WATERWORTH W M, DRURY G E, BRAY C M, et al. Repairing breaks in the plant genome: the importance of keeping it together[J]. New Phytologist, 2011, 192(4):805-822. doi: 10.1111/j.1469-8137.2011.03926.x
[23]	DONG C B, SUO Y J, KANG X Y.Assessment of the genetic composition of triploid hybrid Populus using SSR markers with low recombination frequencies[J]. Canadian Journal of Forest Research, 2014, 44(7):692-699. doi: 10.1139/cjfr-2013-0360
[24]	SCHUELK E M.An economic method for the fluorescent labeling of PCR fragments[J].Nature Biotechnology, 2000, 18(2):233-234. doi: 10.1038/72708
[25]	DONG C B, SUO Y J, WANG J, et al.Analysis of transmission of heterozygosity by 2n gametes in Populus (Salicaceae)[J].Tree Genetics & Genomes, 2015, 11(1):1-7. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=2aea6d20db6378191899d84a3fafe7cc
[26]	MENDIBURU A O, PELOQUIN S J. Bilateral sexual polyploidization in potatoes[J]. Euphytica, 1977, 26(3): 573-583. doi: 10.1007/BF00021683
[27]	BARONE A, GEBHARDT C, FRUSCIANTE L. Heterozygosity in 2n gametes of potato evaluated by RFLP markers[J]. Throret Appl Genet, 1995, 91(1):98-104. doi: 10.1007/BF00220864
[28]	VINING K J, POMRANING K R, WILHELM L J, et al. Dynamic DNA cytosine methylation in the Populus trichocarpa genome: tissue-level variation and relationship to gene expression[J]. BMC Genomics, 2012, 13(1):1-19. doi: 10.1186/1471-2164-13-1
[29]	XI X J, LI D, XU W, et al. 2n egg formation in Populus×euramericana (Dode) Guinier[J].Tree Genetics & Genomes, 2012, 8(6):1237-1245. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=275a9d7517719b3a8b924b3a12047fb7
[30]	康向阳.毛白杨细胞遗传与三倍体选育的研究[D].北京: 北京林业大学, 1996. http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=Y213968 KANG X Y.Study on cytogenetic and allotriploid breeding of Populus tomentosa[D].Beijing: Beijing Forestry University, 1996. http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=Y213968
[31]	康向阳, 朱之悌, 林惠斌.杨树花粉染色体加倍有效处理时期的研究[J].林业科学, 1999, 35(4):21-24. doi: 10.3321/j.issn:1001-7488.1999.04.004 KANG X Y, ZHU Z T, LIN H B.Study on the effective treating period for pollen chromosome doubling of Populu tomentosa×P. bolleana[J]. Scientia Silvae Sinicae, 1999, 35(4):21-24. doi: 10.3321/j.issn:1001-7488.1999.04.004
[32]	康向阳, 朱之悌, 张志毅.银腺杨与毛新杨正反交三倍体选育[J].北京林业大学学报, 2000, 22(6): 8-11. doi: 10.3321/j.issn:1000-1522.2000.06.003 KANG X Y, ZHU Z T, ZHANG Z Y. Breeding of triploids by the reciprocal crossing of Populus alba×P.glandulosa and P.tomentosa×P.bolleana[J]. Journal of Beijing Forestry University, 2000, 22(6):8-11. doi: 10.3321/j.issn:1000-1522.2000.06.003
[33]	李艳华, 康向阳.诱导大孢子染色体加倍选育白杨杂种三倍体[J].北京林业大学学报, 2007, 29(5):22-25. doi: 10.3321/j.issn:1000-1522.2007.05.007 LI Y H, KANG X Y. Triploid induction in white poplar bychromosome doubling of megaspore[J].Journal of Beijing Forestry University, 2007, 29(5):22-25. doi: 10.3321/j.issn:1000-1522.2007.05.007
[34]	王君.青杨派树种多倍体诱导技术研究[D].北京: 北京林业大学, 2009. http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=Y1489837 WANG J.Techniques of polyploid induction in Populus spp. (Section Tacamahaca)[D].Beijing: Beijing Forestry University, 1996. http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=Y1489837
[35]	LU M, ZHANG P D, KANG X Y. Induction of 2n female gametes in Populus adenopoda Maxim. by high temperature exposure during female gametophyte development[J]. Breeding Science, 2013, 63:96-103. doi: 10.1270/jsbbs.63.96
[36]	康宁, 白凤莹, 张平冬, 等.高温诱导胚囊染色体加倍获得毛白杨杂种三倍体[J].北京林业大学学报, 2015, 37(2) : 79-86. doi: 10.13332/j.cnki.jbfu.2015.02.021 KANG N, BAI F Y, ZHANG P D, et al. Inducing chromosome doubling of embryo sac in Populus tomentosa with high temperature exposure for hybrid triploids[J].Journal of Beijing Forestry University, 2015, 37(2):79-86. doi: 10.13332/j.cnki.jbfu.2015.02.021

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Origin identification of 2n female gamete of Populus tomentosa triploid hybrids

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