ObjectiveGenetic diversity of germplasm resources of Leuce was studied to provide reference for the preservation, evaluation and utilization of the germplasm resources of Poplus tomentosa.
MethodIn this study, capillary electrophoresis with fluorescent-labeled primers was used to analyze clones in Leuce, and genetic diversity and genetic variation were analyzed based on the results.
ResultA total of 106 alleles were detected in 272 clones using 16 pairs of SSR primers. The number of alleles per locus ranged from 4 to 11, with an average of 6.625 each loci, the average Ho and He were 0.561 and 0.432, respectively, indicating that the genetic diversity was relatively abundant, and the ratio of Ho to He for 9 loci was greater than 1, indicating the high heterozygosity. In addition, the genetic variation of 234 clones from six concentrated areas of Populus tomentosa was analyzed. The results showed that only 2% of the genetic variation originated among provenances, and 98% within provenances. It could be got to know from the allelic models of different provenances that there were private alleles in five provenances of Shanxi Province, Shaanxi Province, Henan Province, Shandong Province and Hebei Province.Clones with private alleles from different provenances should be selected as parents to broaden the genetic basis of hybrids, or introduced appropriatly to increase the genetic diversity of P. tomentosa among provenances.
ConclusionCluster analysis and principal coordinate analysis showed that the phylogenetic relationship of Beijing and Hebei clones was the closest, and the relationship between Henan and Shanxi, Shandong and Shanxi was closer, respectively. However, clones from the same provenance weren't converged fully one category, owing to genetic variation or the mutual introduction of the provenances. The results provided theoretical basis for the preservation and utilization of germplasm resources of P. tomentosa.
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