<i>Populus euphratica</i> miR1444b positively regulates plants response to drought stress in <i>Arabidopsis thaliana</i>

Li Shuang; Su Yanyan; Wang Houling; Li Huiguang; Liu Chao; Xia Xinli; Yin Weilun

doi:10.13332/j.1000-1522.20180043

Journal of Beijing Forestry University > 2018 > 40(4): 1-9. > DOI: 10.13332/j.1000-1522.20180043

Li Shuang, Su Yanyan, Wang Houling, Li Huiguang, Liu Chao, Xia Xinli, Yin Weilun. Populus euphratica miR1444b positively regulates plants response to drought stress in Arabidopsis thaliana[J]. Journal of Beijing Forestry University, 2018, 40(4): 1-9. DOI: 10.13332/j.1000-1522.20180043

Citation:

PDF (5006 KB)

Populus euphratica miR1444b positively regulates plants response to drought stress in Arabidopsis thaliana

College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China

More Information

Received Date: January 29, 2018
Revised Date: March 15, 2018
Published Date: March 31, 2018

Graphical Abstract

Abstract

Abstract

Objective miR1444b is a member of woody plant specific miR1444 family participating in plant response to metal stress. However, its function in response to drought stress is unknown.
Method We isolated Populus euphratica MIR1444b gene (peu-MIR1444b) and its promoter (pro-peu-MIR1444b), then overexpressed peu-MIR1444b gene at the model plant Arabidopsis thaliana. The peu-MIR1444b gene's function was analyzed under normal watering, mannitol-simulated drought and natural soil drought conditions.
Result We compared the genomic sequence homology of Populus trichocarpa collected from the Phytozome database with the pro-peu-MIR1444b and peu-MIR1444b, which sharing 99.61% and 98.82% similarity, respectively. Analysis using the PlantCARE database revealed that the pro-peu-MIR1444b sequence contained the stress-related TC-rich repeat elements and the drought-induced MYB binding site MBS. QRT-PCR results showed that transgenic lines had significantly higher expression level of miR1444b than in wild type, the expression level of target gene glucan synthase-like Ⅳ (AT3G14570.2) was down-regulated, and it indicated that glucan synthase-like Ⅳ was negatively regulated by peu-miR1444b in A. thaliana. Under 250 mmol/L mannitol-simulated drought stress condition, germination rate of TG-2 and TG-5 in transgenic lines was significantly higher than wild-type by 20.83% and 26.67%, and their root length was drastically higher than wild-type by 56.83% and 52.60%(P < 0.05), respectively. At 8 days after natural soil drought treatment, photosynthetic rate, stomatal conductance, PSⅡ maximum photochemical efficiency, leaf water use efficiency and peroxidase (POD) activity in transgenic lines were obviously higher than those in wild type (P < 0.05).
Conclusion Our study reveals that miR1444b increases water absorption capacity of plants by promoting root growth, negative regulation of glucan synthase-like Ⅳ enhance plant osmotic adjustment ability to maintain plant photosynthetic efficiency under water deficit environment, ensure the normal growth of plants. miR1444b positively regulates plant response to drought stress in A. thaliana.
- Populus euphratica,
- miR1444b,
- drought,
- mannitol,
- photosynthetic rate

FullText(HTML)

References (29)

References

[1]	王让会, 王晓伟, 游先祥, 等.荒漠河岸林生态系统的结构分析[J].干旱区研究, 2002, 19(2):7-11. http://d.old.wanfangdata.com.cn/Periodical/ghqyj200202002 Wang R H, Wang X W, You X X, et al.Analysis on the structure of the desert riparian forest ecosystems[J].Arid Zone Research, 2002, 19(2):7-11. http://d.old.wanfangdata.com.cn/Periodical/ghqyj200202002
[2]	Dong Y, Wang C, Han X, et al.A novel bHLH transcription factor PeHLH35 from Populus euphratica confers drought tolerance through regulating stomatal development, photosynthesis and growth in Arabidopsis[J].Biochemical & Biophysical Research Communications, 2014, 450(1):453-458.
[3]	He F, Wang H L, Li H G, et al.PeCHYR1, a ubiquitin E3 ligase from Populus euphratica, enhances drought tolerance via ABA-induced stomatal closure by ROS production in Populus[J/OL].Plant Biotechnology Journal, 2018[2018-03-09].https://doi.org/10.1111/pbi.12893.
[4]	李岚, 王厚领, 赵琳, 等.异源表达Peu-miR473a增强拟南芥的抗旱性[J].北京林业大学学报, 2015, 37(5):30-39. doi: 10.13332/j.1000-1522.20140461 Li L, Wang H L, Zhao L, et al.Heterogeneous expression of Peu-miR473a gene confers drought tolerance in Arabidopsis thaliana[J].Journal of Beijing Forestry University, 2015, 37(5):30-39. doi: 10.13332/j.1000-1522.20140461
[5]	段中鑫.胡杨microRNA Peu-miR156j和Peu-miR69o表达模式分析及功能鉴定[D].北京: 北京林业大学, 2012. Duan Z X.Expression pattern and functional analysis of microRNA peu-miR156j and peu-miR169o from Populous euphratica [D].Beijing: Beijing Forestry University, 2012.
[6]	段中鑫, 覃玉蓉, 夏新莉, 等.超量表达胡杨peu-MIR156j基因增强拟南芥耐盐性[J].北京林业大学学报, 2011, 33(6):1-7. http://j.bjfu.edu.cn/article/id/9665 Duan Z X, Qin Y R, Xia X L, et al.Overexpression of Populous euphratica peu-miR156j gene enhancing salt tolerance in Arabidopsis thaliana[J].Journal of Beijing Forestry University, 2011, 33(6):1-7. http://j.bjfu.edu.cn/article/id/9665
[7]	Park W, Li J, Song R, et al.Carpel factory, a dicer homolog, and HEN1, a novel protein, act in microRNA metabolism in Arabidopsis thaliana[J].Current Biology, 2002, 12(17):1484-1495. doi: 10.1016/S0960-9822(02)01017-5
[8]	Zhang Y C, Yu Y, Wang C Y, et al.Overexpression of microRNA OsmiR397 improves rice yield by increasing grain size and promoting panicle branching[J].Nature Biotechnology, 2013, 31(9):848-854. doi: 10.1038/nbt.2646
[9]	Taylor P F, Guiling S, Caitlin E B, et al.Salt and drought stresses induce the aberrant expression of microRNA genes in tobacco[J].Molecular Biotechnology, 2011, 49(2):159-165. doi: 10.1007/s12033-011-9387-5
[10]	Li B S, Qin Y R, Duan H, et al.Genome-wide characterization of new and drought stress responsive microRNAs in Populus euphratica[J].Journal of Experimental Botany, 2011, 62 (11):3765-3779. doi: 10.1093/jxb/err051
[11]	Wang T Z, Chen L, Zhao M, et al.Identification of drought-responsive microRNAs and their targets in Medicago truncatula by genome-wide high-throughput sequencing[J].BMC Genomics, 2011, 12(1):361-372. doi: 10.1186/1471-2164-12-361
[12]	Lu X, Duan H, Lian C L, et al.The role of peu-miR164 and its target Pe-NAC genes in response to abiotic stress in Populus euphratica[J].Plant Physiology & Biochemistry, 2017, 115:418-438.
[13]	Lu S F, Chiang C.Conservation and diversity of MicroRNA-associated copper-regulatory networks in Populous trichocarpa [J].Journal of Integrative Plant Biology, 2011, 53(11):879-891. doi: 10.1111/jipb.2011.53.issue-11
[14]	崔秀娜.毛果杨微小RNA1444a的克隆、胁迫表达分析及遗传转化研究[D].阜新: 辽宁工程技术大学, 2012. http://cdmd.cnki.com.cn/Article/CDMD-10147-1014181640.htm Cui X N.Cloning, stress expression analysis and genetic transformation of miR1444a in Populus trichacarpa[D].Fuxin: Liaoning University of Engineering and Technology, 2012. http://cdmd.cnki.com.cn/Article/CDMD-10147-1014181640.htm
[15]	覃玉蓉.胡杨microRNAs的克隆及其功能研究[D].北京: 北京林业大学, 2011. http://cdmd.cnki.com.cn/Article/CDMD-10022-1011134964.htm Qin Y R.Cloning and functional analysis of microRNAs from Populus euphratica[D].Beijing: Beijing Forestry University, 2011. http://cdmd.cnki.com.cn/Article/CDMD-10022-1011134964.htm
[16]	Zhang X, Henriques R, Lin S S, et al.Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method[J].Nature Protocols, 2006, 1 (2):641-646. doi: 10.1038/nprot.2006.97
[17]	Shi H T, Ye T, Zhu J K, et al.Constitutive production of nitric oxide leads to enhanced drought stress resistance and extensive transcriptional reprogramming in Arabidopsis[J].Journal of Experimental Botany, 2014, 65(15):4119-4131. doi: 10.1093/jxb/eru184
[18]	Si L, Guo C, Cao Y, et al.The effect of nitrobenzene on antioxidative enzyme activity and DNA damage in tobacco seedling leaf cells[J].Environmental Toxicology & Chemistry, 2012, 31 (9):2078-2084. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=1e4cd3977ce9520946e32365fbfc39de
[19]	高润石, 王红, 高艾.茎环法与加PolyA尾法PCR在检测MicroRNA时引物设计的策略[J].毒理学杂志, 2012, 26(5):378-381. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=wsdlxzz201205016 Gao R S, Wang H, Gao A.Strategy of primer design for detecting microRNA by stem loop method and PolyA tail PCR[J].Toxicology Journal, 2012, 26(5):378-381. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=wsdlxzz201205016
[20]	Rao G, Sui J, Zeng Y, et al.Denovo transcriptome and small RNA analysis of two Chinese willow cultivars reveals stress response genes in Salix matsudana [J/OL].PLos One, 2014, 9(10): e109122[2017-10-06]. https://doi.org/10.1371/journal.pone.0109122.
[21]	Song Y, Ma K, Ci D, et al.Sexual dimorphism floral microRNA profiling and target gene expression in andromonoecious poplar (Populus tomentosa)[J/OL].PLos One, 2013, 8(5): e62681[2017-10-06]. http://doi.org/10.1371/journal.pone.0062681.
[22]	Chen L, Yuan R, Zhang Y, et al.Genome-wide profiling of novel and conserved Populus microRNAs involved in pathogen stress response by deep sequencing[J].Planta, 2012, 235(5):873-883. doi: 10.1007/s00425-011-1548-z
[23]	Wang M Z, Li C L, Lu S F.Origin and evolution of miR1444 genes in Salicaceae[J].Scientific Reports, 2017, 7:39740. doi: 10.1038/srep39740
[24]	杨青杰.黄龙胆类胡萝卜素生物合成基因启动子的功能分析[D].哈尔滨: 东北师范大学, 2013. http://cdmd.cnki.com.cn/Article/CDMD-10200-1013357801.htm Yang Q J.Functional characterization of the Gentiana lutea carotenoid biosynthetic gene promoters[D].Harbin: Northeast Normal University, 2013. http://cdmd.cnki.com.cn/Article/CDMD-10200-1013357801.htm
[25]	Youshihiro N, Kazuo N, Zabta K S, et al.Interaction between two cis-acting elements, ABRE and DRE, in ABA-dependent expression of Arabidopsis rd29A gene in response to dehydration and high-salinity stresses[J].Plant Journal for Cell & Molecular Biology, 2003, 34(2):137-148. doi: 10.1046/j.1365-313X.2003.01708.x
[26]	Gao S, Gao J, Zhu X, et al.ABF2, ABF3, and ABF4 promote ABA-mediated chlorophyll degradation and leaf senescence by transcriptional activation of chlorophyll catabolic genes and senescence-associated genes in Arabidopsis[J].Molecular Plant, 2016, 9(9):1272-1285. doi: 10.1016/j.molp.2016.06.006
[27]	石洪萍.三角褐指藻尿苷二磷酸葡萄糖焦磷酸化酶在碳流分配中的功能[D].青岛: 中国海洋大学, 2015. http://cdmd.cnki.com.cn/Article/CDMD-10423-1015717108.htm Shi H P.Carbon allocation function of UDP-glucose pyrophosphorylase in Pheodactylum tricornutum [D].Qingdao: Ocean University of China, 2015. http://cdmd.cnki.com.cn/Article/CDMD-10423-1015717108.htm
[28]	Ute H, Hans W, Helmut B.A sucrose-synthase gene of Vicia faba L. :expression pattern in developing seeds in relation to starch synthesis and metabolic regulation[J].Planta, 1993, 191(3):394-401. https://www.ncbi.nlm.nih.gov/pubmed/7764025
[29]	Zhang J X, Kirkham M B.Mechanisms of chilling-induced oxidative stress injury and tolerance in developing maize seedlings:changes in antioxidant system, oxidation of proteins and lipids, and protease activities[J].The Plant Journal, 1994, 35(5):785-791.

Cited By

Cited by

Periodical cited type(9)

1.	ZHANG Kai，ZHANG Hailong，TIAN Jianjin，QU Jianjun，ZHANG Xingxin，WANG Zhenghui，XIAO jianhua. Flow field, sedimentation, and erosion characteristics around folded linear HDPE sheet sand fence: Numerical simulation study. Journal of Mountain Science. 2024(01): 113-130 .
2.	马泽，蒙仲举，罗建国，阿拉腾陶格苏，赵东颖. 乌兰布和沙漠典型沿黄段格状沙障防风固沙效应. 水土保持通报. 2024(02): 1-10+21 .
3.	闫德仁，黄海广，闫婷. 纱网沙障研究进展. 内蒙古林业科技. 2024(03): 50-53 .
4.	潘加朋，张克存，安志山，张宏雪，薛承杰. 基于风洞模拟试验的典型机械防沙工程防护效益对比. 水土保持学报. 2023(06): 104-110 .
5.	张帅，原伟杰，刘卉，王海霞，官昊慧，王鹿振. 附面层位移厚度对沙障防风效益评价的适用性研究——以聚乳酸（PLA）沙障为例. 干旱区地理. 2023(12): 1973-1983 .
6.	李双立，黄海广，党晓宏，郭跃，万俊华. 基于CNKI数据库文献计量视角下沙障研究现状. 防护林科技. 2022(05): 75-78+85 .
7.	韩雪莹，王涛，杨文斌，贾光普，刘静，杨宇. 中国沙障研究进展与热点分析：基于Vosviewer和Citespace的图谱量化分析. 中国沙漠. 2021(02): 153-163 .
8.	祁帅，汪季，党晓宏，魏亚娟，丁奋谦. 3种低立式沙障内表层沉积物粒度特征研究. 干旱区研究. 2021(03): 875-881 .
9.	厉静文，Dosmanbetov D A，郭浩，辛智鸣，刘朋飞，刘明虎. 不同配置乔灌混交林防风效益的风洞试验. 农业工程学报. 2020(11): 95-102 .

Other cited types(7)

Get Citation

PDF

XML

Article views (2560) PDF downloads (208) Cited by(16)

Turn off MathJax

Article Contents

Abstract

References

Populus euphratica miR1444b positively regulates plants response to drought stress in Arabidopsis thaliana

Abstract

References

Cited by

Periodical cited type(9)

Other cited types(7)

Catalog

Export File

Citation

Format

Content