Cloning and expression characteristics analysis of <i>BpTCP</i>2 gene in <i>Betula platyphylla</i>

Luan Jiayu; An Linjun; Ren Li; Li Huiyu

doi:10.13332/j.1000-1522.20180430

Journal of Beijing Forestry University > 2019 > 41(8): 57-66. > DOI: 10.13332/j.1000-1522.20180430

Luan Jiayu, An Linjun, Ren Li, Li Huiyu. Cloning and expression characteristics analysis of BpTCP2 gene in Betula platyphylla[J]. Journal of Beijing Forestry University, 2019, 41(8): 57-66. DOI: 10.13332/j.1000-1522.20180430

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Cloning and expression characteristics analysis of BpTCP2 gene in Betula platyphylla

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, Heilongjiang, China

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Received Date: December 24, 2018
Revised Date: April 15, 2019
Available Online: July 02, 2019
Published Date: July 31, 2019

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Abstract

Abstract

ObjectiveThe BpTCP2 gene was cloned, sequence characteristics were analyzed, and the gene expression characteristics of the gene under different tissues, hormone treatments and abiotic stress were detected, which provided a reference for revealing the function of BpTCP2 in plant growth and development and stress response.
MethodThe full-length cDNA sequence of BpTCP2 gene was cloned by PCR technology and its cDNA sequence characteristics were analyzed by bioinformatics. Real-time quantitative PCR was used to analyze expression level of the gene in different tissues and the responses of exogenous phytohormones (ABA, IAA, BR, JA, SA) and abiotic stress (CdCl₂, NaCl, NaHCO₃, PEG).
ResultThe full-length cDNA sequence of BpTCP2 gene was successfully cloned. Bioinformatics analysis found that BpTCP2 contained a highly conserved bHLH domain and BpTCP2 belongs to TCP-P subclass; qRT-PCR analysis showed that the expression of BpTCP2 gene was higher in xylem, young leaves and stem segments. Under the treatment of CdCl₂, NaCl and NaHCO₃, the gene was up-regulated. Five exogenous phytohormones could induce the expression of the gene.
ConclusionThe BpTCP2 gene was involved in the development of stems of Betula platyphylla, and affects the development of leaves and early stem segments and responds to various hormones and heavy metals, salts and alkalis.
- Betula platyphylla,
- BpTCP2,
- bioinformatics,
- gene cloning,
- expression characteristics

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