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WANG Wen-qi, GAI Ying, LU Hai, JIANG Xiang-ning. Recombinase Cre's overexpression in Escherichia coli BL21(DE3) and its one-step purification and activity assay[J]. Journal of Beijing Forestry University, 2006, 28(3): 57-60.
Citation: WANG Wen-qi, GAI Ying, LU Hai, JIANG Xiang-ning. Recombinase Cre's overexpression in Escherichia coli BL21(DE3) and its one-step purification and activity assay[J]. Journal of Beijing Forestry University, 2006, 28(3): 57-60.
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Recombinase Cre's overexpression in Escherichia coli BL21(DE3) and its one-step purification and activity assay

  • College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, P. R. China

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  • Received Date: March 19, 2005
  • Available Online: May 14, 2024
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  • In order to realize the application of Cre recombinase in vitro,plasmid pTE30-Cre was constructed based on a pET-30a vector.This plasmid was transferred into Escherichia coli BL21(DE3).The Cre gene was overexpressed and then induced by IPTG.The Cre recombinase protein was easily purified using a Ni+ affinity column and its activity was tested in vitro by providing a plasmid which contained two of the same directional loxP sites.
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