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覆盖对城市裸露土壤真菌多样性及群落结构的影响

范志辉 李素艳 孙向阳 曲炳鹏 郑燚 周伟 杜田甜

范志辉, 李素艳, 孙向阳, 曲炳鹏, 郑燚, 周伟, 杜田甜. 覆盖对城市裸露土壤真菌多样性及群落结构的影响[J]. 北京林业大学学报. doi: 10.12171/j.1000-1522.20210129
引用本文: 范志辉, 李素艳, 孙向阳, 曲炳鹏, 郑燚, 周伟, 杜田甜. 覆盖对城市裸露土壤真菌多样性及群落结构的影响[J]. 北京林业大学学报. doi: 10.12171/j.1000-1522.20210129
Fan Zhihui, Li Suyan, Sun Xiangyang, Qu Bingpeng, Zheng Yi, Zhou Wei, Du Tiantian. Effects of mulching on fungal diversity and community structure in urban exposed soil[J]. Journal of Beijing Forestry University. doi: 10.12171/j.1000-1522.20210129
Citation: Fan Zhihui, Li Suyan, Sun Xiangyang, Qu Bingpeng, Zheng Yi, Zhou Wei, Du Tiantian. Effects of mulching on fungal diversity and community structure in urban exposed soil[J]. Journal of Beijing Forestry University. doi: 10.12171/j.1000-1522.20210129

覆盖对城市裸露土壤真菌多样性及群落结构的影响

doi: 10.12171/j.1000-1522.20210129
基金项目: 北京市自然科学基金(6202021);科技基础资源调查专项(2021FY100802)
详细信息
    作者简介:

    范志辉

    责任作者:

    李素艳

Effects of mulching on fungal diversity and community structure in urban exposed soil

  • 摘要:   目的  本试验旨在探究不同覆盖材料对土壤真菌多样性及群落结构的影响。  方法  通过控制实验,运用Illumina MiSeq高通量测序技术对不同覆盖材料下的土壤真菌群落进行测定,并对其进行多样性和群落结构的分析。  结果  6种覆盖材料中,树皮处理下的土壤真菌群落的物种多样性和丰富度最高,以脲醛树脂为胶黏剂制备的有机覆盖垫处理下的土壤真菌多样性和丰富度最低。不同覆盖处理的土壤中,真菌物种和优势物种存在差异。在门水平上,子囊菌门为最优势菌门,相对丰度为70.84% ~ 86.86%,远远大于次优势的担子菌门(相对丰度为3.27% ~ 12.78%)。在属水平上,有机覆盖垫、鹅卵石、陶粒、树皮以及无覆盖处理中unclassified_o_Hypocreales为最优势属,在木片覆盖处理中,最优势属为毛壳菌属,在园林绿化废弃物覆盖处理中unclassified_o_Eurotiales为最优势属。相关性分析结果显示,土壤速效磷含量与土壤真菌多样性呈极显著负相关(P < 0.01),与土壤真菌丰富度呈显著负相关(P < 0.05);土壤速效磷含量与土壤真菌多样性呈显著负相关(P < 0.05),与土壤有机质含量呈极显著负相关(P < 0.01)。毛壳菌属、norank_p_Mucoromycota、unclassified_f_Pyronemataceae、镰刀菌属受环境因子影响较大。  结论  覆盖措施能够影响土壤真菌多样性和丰富度以及群落结构,不同覆盖材料对土壤真菌群落的物种多样性和丰富度以及群落结构影响不同。土壤速效磷、有机质含量是影响土壤真菌多样性和丰富度的重要因素,不同物种对于环境因子的响应也存在差异。本研究设置的6种覆盖材料中,使用树皮作为覆盖物处理下的土壤真菌多样性和丰富度最高。

     

  • 图  1  Pan/Core物种分析

    Figure  1.  Pan/Core species analysis

    图  2  不同处理在门水平上的优势物种

    Figure  2.  Dominant species of different treatments at the Phylum level

    图  3  不同处理在属水平上的优势物种

    Figure  3.  Dominant species of different treatments at the genus level

    图  4  环境因子与属水平上真菌群落的相关性

    Figure  4.  Correlations between environmental factors and fungal communities at the genus level

    表  1  不同覆盖物下的土壤化学性质

    Table  1.   Soil chemical properties of different mulches

    处理
    Treatment
    碱解氮
    AN/(mgˑkg−1
    速效磷
    AP/(mgˑkg−1
    速效钾
    AK(mgˑkg−1
    有机质
    SOM(gˑkg−1
    pH
    CK44.90 ± 0.71de14.25 ± 0.27f219.80 ± 1.28b15.61 ± 0.34c8.36 ± 0.17b
    NQ53.15 ± 0.70a21.44 ± 0.38a266.25 ± 1.74a17.80 ± 0.25a8.24 ± 0.09c
    Y50.66 ± 0.65ab19.32 ± 0.21b265.77 ± 1.20a18.39 ± 0.37a8.18 ± 0.14c
    M47.32 ± 0.61cd17.55 ± 0.30d259.08 ± 3.44a16.38 ± 0.35b8.27 ± 0.13bc
    S49.16 ± 0.61bc18.27 ± 0.27c260.09 ± 1.52a13.17 ± 0.28e8.50 ± 0.12a
    T44.17 ± 0.59e14.69 ± 0.33f218.03 ± 1.38b15.57 ± 0.33c8.54 ± 0.09a
    E40.64 ± 0.69f15.59 ± 0.29e199.43 ± 1.00b14.51 ± 0.37d8.36 ± 0.13b
    注:数据表示形式均为平均值 ± 标准误,表中小写字母表示不同覆盖物下间的差异显著(P < 0.05)。Notes: data representations are mean ± standard error. The small letters in the table indicate significant differences in mulches (P < 0.05).
    下载: 导出CSV

    表  2  不同覆盖物下的土壤真菌群落的多样性和丰富度

    Table  2.   Diversity and richness of soil fungal communities with different mulches

    处理 TreatmentOTU数 OUT numberShannon 指数 Shannon indexChao1 指数 Chao1 index覆盖率
    Coverage/%
    CK2103.22 ± 0.08 a248.19 ± 7.93 a99.88
    NQ1272.13 ± 0.03 b167.33 ± 4.91 c99.93
    Y1472.67 ± 0.15 a192.32 ± 2.12 b99.87
    M1762.75 ± 0.23 a207.74 ± 8.69 b99.90
    S1973.09 ± 0.05 a242.70 ± 6.91 a99.88
    T1692.93 ± 0.07 a195.60 ± 11 b99.90
    E1642.79 ± 0.32 a195.80 ± 8.49 b99.92
    注:数据表示形式均为平均值 ± 标准误,表中小写字母表示不同覆盖物下的差异显著(P < 0.05)。Notes: the data representations are mean ± standard error. The small letters in the table indicate significant differences in mulches (P < 0.05).
    下载: 导出CSV

    表  3  不同覆盖物下的土壤真菌的多样性和丰富度与土壤化学性质的相关性

    Table  3.   Correlations between the diversity and richness of soil fungi under different mulches and soil chemical properties

    相关性
    Relevance
    碱解氮
    AN
    速效磷
    AP
    速效钾
    AK
    有机质
    SOM
    pH
    Shannon 指数 Shannon index −0.430 −0.663** −0.401 −0.493* 0.392
    Chao1 指数 Chao1 index −0.242 −0.488* −0.185 −0.571** 0.327
    注:*、**分别表示指数与土壤化学性质在P < 0.05、P < 0.01水平显著相关。Notes: * and ** indicate significant correlations at P < 0.05 and P < 0.01 levels between index and the chemistry of soil, respectively.
    下载: 导出CSV

    表  4  不同覆盖物下的各分类水平特有类群

    Table  4.   Various levels of endemic species with different mulches

    PlylumClassOrderFamilyGenusSpecies
    CK c__
    Archaeorhizomycetes
    古根菌纲
    o__
    Archaeorhizomycetales
    古根菌目
    f__
    Archaeorhizomycetaceae
    古根菌科
    g__Archaeorhizomyces
    古根菌属
    s__unclassified_g__
    Archaeorhizomyces
    o__Paraglomerales
    类球囊霉纲
    f__Paraglomeraceae
    类球囊霉科
    g__Paraglomus
    类球囊霉属
    s__Paraglomus_occultum
    隐球囊霉
    s__Conidiobolus_lamprauges
    s__Catenaria_anguillulae
    线虫链枝菌
    S o__unclassified_c__
    Eurotiomycetes
    f__unclassified_c__
    Eurotiomycetes
    g__unclassified_c__
    Eurotiomycetes
    s__unclassified_c__
    Eurotiomycetes
    g__Sugitazyma s__Sugitazyma_miyagiana
    g__Tritirachium
    麦轴梗霉属
    s__unclassified_g__
    Tritirachium
    NQ
    M
    E
    T
    Y
    下载: 导出CSV
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  • 录用日期:  2022-07-08
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