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低磷胁迫对水曲柳幼苗光合速率与氮素同化的影响

杨博文 孙海龙 吴楚

杨博文, 孙海龙, 吴楚. 低磷胁迫对水曲柳幼苗光合速率与氮素同化的影响[J]. 北京林业大学学报, 2015, 37(8): 18-23. doi: 10.13332/j.1000-1522.20140417
引用本文: 杨博文, 孙海龙, 吴楚. 低磷胁迫对水曲柳幼苗光合速率与氮素同化的影响[J]. 北京林业大学学报, 2015, 37(8): 18-23. doi: 10.13332/j.1000-1522.20140417
YANG Bo-wen, SUN Hai-long, WU Chu. Effects of phosphorus stress on photosynthesis and nitrogen assimilation of Fraxinus mandshurica seedlings[J]. Journal of Beijing Forestry University, 2015, 37(8): 18-23. doi: 10.13332/j.1000-1522.20140417
Citation: YANG Bo-wen, SUN Hai-long, WU Chu. Effects of phosphorus stress on photosynthesis and nitrogen assimilation of Fraxinus mandshurica seedlings[J]. Journal of Beijing Forestry University, 2015, 37(8): 18-23. doi: 10.13332/j.1000-1522.20140417

低磷胁迫对水曲柳幼苗光合速率与氮素同化的影响

doi: 10.13332/j.1000-1522.20140417
基金项目: 

十二五”

国家科技支撑计划项目(2012BAD21B0202)

详细信息
    作者简介:

    杨博文.主要研究方向:园林树木学。Email: ybw421294259@163.com 地址:150040 黑龙江省哈尔滨市和兴路26号东北林业大学园林学院。

    责任作者:

    孙海龙,讲师。主要研究方向:树木生理、森林培育。Email: shlong12@126.com 地址:150040 黑龙江省哈尔滨市和兴路26号东北林业大学林学院。

Effects of phosphorus stress on photosynthesis and nitrogen assimilation of Fraxinus mandshurica seedlings

  • 摘要: 在温室内采用盆栽砂培的方法,对不同供磷水平(0.125、0.250、0.500和1.000mmol/L)的水曲柳幼苗进行了光合速率与氮素同化的研究。结果表明,水曲柳幼苗最大净光合速率随供磷减少而降低,供磷水平最低(0.125mmol/L)时水曲柳幼苗叶片最大净光合速率为正常供磷(1mmol/L)的61%,而低磷水平处理(0.125、0.250、0.500mmol/L)时的光补偿点均高于正常供磷处理。各供磷水平下,叶片中的全氮、硝态氮和铵态氮含量均高于根系。随供磷的减少,叶片与根系中硝态氮含量均降低,同时,低磷水平处理(0.125、0.250、0.500mmol/L)时根系铵态氮含量均高于正常供磷(1mmol/L),表明低磷胁迫下水曲柳偏向铵态氮的吸收。但是,叶片全氮含量随供磷水平的降低而下降。供磷水平较低时(0.125和0.25mmol/L)叶片硝酸还原酶(NR)活性低于根系,而在供磷水平较高时(0. 5和1mmol/L)则相反。随供磷的减少,叶片和根系NR活性,以及叶片天冬氨酸转氨酶(AAT)活性均逐渐降低,而叶片谷氨酰胺合成酶(GS)活性变化较小。上述结果表明,在低磷胁迫下水曲柳幼苗氮素吸收数量和同化酶活性均降低,减少了氮素向叶片的分配,导致植株光合速率下降。

     

  • [1] GRACIANO C, TAMBUSSI E, CASTÁN E, et al. Dry mass partitioning and nitrogen uptake by Eucalyptus grandis plants in response to localized or mixed application of phosphorus[J]. Plant and Soil, 2009, 319(1-2): 175-184.
    [1] YUAN X Y,GUO P Y,HUANG J,et al. Effect of glyphosate on photosynthesis and chlorophyll fluorescence of leaves of glyphosate-resistant soybean( Glycine max ( L.) Merr.)seedlings under phosphorus deficiency stress[J]. Journal of Plant Nutrition and Fertilizer, 2014, 20(1): 221-228.
    [2] OLIVERA M, TEJERA N, IRIBARNE C, et al. Growth, nitrogen fixation and ammonium assimilation in common bean ( Phaseolus vulgaris ): effect of phosphorus[J]. Physiologia Plantarum, 2004, 121(3): 498-505.
    [2] GUO S L,YAN X F,BAI B,et al. Responses of larch seedling's photosynthetic characteristics to nitrogen and phosphorus deficiency[J]. Chinese Journal of Applied Ecology, 2005, 16(4): 589-594.
    [3] FAN W G,WANG L X. Photosynthetic response to different phosphorus levels on young Newhall navel orange trees[J]. Journal of Fruit Science, 2012, 29(2): 166-170.
    [3] WARREN C R, ADAMS M A. Phosphorus affects growth and partitioning of nitrogen to Rubisco in Pinus pinaster [J]. Tree Physiology, 2002, 22(1): 11-19.
    [4] WU C,WANG Z Q,SUN H L,et al. Effects of different concentrations of nitrogen and phosphorus on chlorophyll biosynthesis, chlorophyll a fluorescence, and photosynthetic rate in Larix olgensis seedlings[J]. Scientia Silvae Sinicae, 2005, 41(4): 31-36.
    [4] BOWN H E, WATT M S, CLINTON T W, et al. Partititioning concurrent influences of nitrogen and phosphorus supply on photosynthetic model parameters of Pinus radiata [J]. Tree Physiology, 2007, 27(3): 335-344.
    [5] BLOOMFIELD K J, FARQUHAR G D, LLOYD J. Photosynthesis-nitrogen relationships in tropical forest tree species as affected by soil phosphorus availability: a controlled environment study[J]. Functional Plant Biology, 2014, 41(8): 820-832.
    [5] WU C,FAN Z Q,WANG Z Q. Effect of phosphorus stress on chlorophyll biosynthesis, photosynthesis and biomass partitioning pattern of Fraxinus mandchurica seedlings[J]. Chinese Journal of Applied Ecology, 2004, 15(6): 935-940.
    [6] REICH P, OLEKSYN J, WRIGHT I. Leaf phosphorus influences the photosynthesis-nitrogen relation: a cross-biome analysis of 314 species[J]. Oecologia, 2009, 160(2): 207-212.
    [6] WU C,FAN Z Q,WANG Z Q. Influences of nitrogen and phosphorus supply on their absorption and growth of Fraxinus mandshurica seedlings[J]. Scientia Silvae Sinicae, 2005, 41(5): 196-200.
    [7] 原向阳,郭平毅,黄洁,等. 缺磷胁迫下草甘膦对抗草甘膦大豆幼苗光合作用和叶绿素荧光参数的影响[J]. 植物营养与肥料学报, 2014, 20(1): 221-228.
    [7] LI H S. Principles and techniques of plant physiological biochemical experiment[M]. Beijing: Higher Education Press, 2000: 56-98.
    [8] WARREN C R. How does P affect photosynthesis and metabolite profiles of Eucalyptus globulus [J]. Tree Physiology, 2011, 31(7): 727-739.
    [8] ZHANG Y D,FAN Z Q,WANG Q C,et al. Effect of different nitrogen forms on growth of Fraxinus mandshurica seedlings[J]. Chinese Journal of Applied Ecology, 2000, 11(10): 665-667.
    [9] 郭盛磊,阎秀峰,白冰,等. 落叶松幼苗光合特性对氮和磷缺乏的响应[J]. 应用生态学报, 2005, 16(4): 589-594.
    [9] ZHANG Y,CUI X Y. Nitrogen absorption and assimilation characteristics of Pinus koraiensis seedlings in different N H 4 + /N O 3 - ratios[J]. Journal of Beijing Forestry University, 2011, 33(5):61-64.
    [10] GUO Y P,CHEN P Z,ZHANG L C,et al. Effects of different phosphorus nutrition levels on photosynthesis in satsuma mandarin ( Citrus unshiu Marc. ) leaves[J]. Plant Nutrition and Fertilizer Science, 2002, 8(2): 186-191.
    [10] 樊卫国,王立新. 纽荷尔脐橙幼树对不同供磷水平的光合响应[J]. 果树学报, 2012, 29(2): 166-170.
    [11] RUFTY T W, MACKOWN C T, ISRAEL D W. Phosphorus stress effects on assimilation of nitrate[J]. Plant Physiology, 1990, 94(1): 328-333.
    [12] GNIAZDOWSKA A, RYCHTER A. Nitrate uptake by bean ( Phaseolus vulgaris L.) roots under phosphate deficiency[J]. Plant and Soil, 2000, 226(1): 79-85.
    [13] BLOOMFIELD K J, FARQUHAR G D, LLOYD J. Photosynthesis-nitrogen relationships in tropical forest tree species as affected by soil phosphorus availability: a controlled environment study[J]. Functional Plant Biology, 2014, 41(8): 820-832.
    [14] 吴楚,王政权,孙海龙,等. 氮磷供给对长白落叶松叶绿素合成、叶绿素荧光和光合速率的影响[J]. 林业科学, 2005, 41(4): 31-36.
    [15] BOYCE R L, LARSON J R, SANFORD R L. Phosphorus and nitrogen limitations to photosynthesis in Rocky Mountain bristlecone pine ( Pinus aristata ) in Colorado[J]. Tree Physiology, 2006, 26(11): 1477-1486.
    [16] WALKER A P, BECKERMAN A P, GU L, et al. The relationship of leaf photosynthetic traits-V cmax and J max - to leaf nitrogen, leaf phosphorus, and specific leaf area: a meta-analysis and modeling study[J]. Ecology and Evolution, 2014, 4(16): 3218-3235.
    [17] TISCHNER R. Nitrate uptake and reduction in higher and lower plants[J]. Plant, Cell and Environment. 2000, 23(10): 1005-1024.
    [18] GRADOWSKI T, THOMAS S C. Phosphorus limitation of sugar maple growth in central Ontario[J]. Forest Ecology and Management, 2006, 226: 104-109.
    [19] RICHARDS A E, FORRESTER D I, BAUHUS J, et al. The influence of mixed tree plantations on the nutrition of individual species: a review[J]. Tree Physiology, 2010, 30 (9): 1192-1208.
    [20] TISSUE D T, LEWIS J D. Photosynthetic responses of cottonwood seedlings grown in glacial through future atmospheric [CO 2 ] vary with phosphorus supply[J]. Tree Physiology, 2010, 30(11): 1361-1372.
    [21] 吴楚,范志强,王政权. 磷胁迫对水曲柳幼苗叶绿素合成、光合作用和生物量分配格局的影响[J]. 应用生态学报, 2004, 15(6): 935-940.
    [22] 吴楚,范志强,王政权. 氮磷供应状态对水曲柳幼苗氮磷吸收与生长的影响[J]. 林业科学, 2005, 41(5): 196-200.
    [23] MR B, ESCOBAR-GUTIÉRREZ A, BURNS A, et al. Nitrogen-limited growth of lettuce is associated with lower stomatal conductance[J]. New Phytologist, 2001, 152: 97-106.
    [24] 李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000: 56-98.
    [25] KNOEPP J D, TURNER D P, TINGEY D T. Effects of ammonium and nitrate on nutrient uptake and activity of nitrogen assimilating enzymes in western hemlock[J].Forest Ecology and Management, 1993, 59: 179-191.
    [26] HUSTED S, HEBBERN C A, MATTSSON M, et al. A critical experimental evaluation of methods for determination of N H 4 + in plant tissue, xylem sap and apoplastic fluid[J]. Physiologia Plantarum, 2000, 109(2): 167-179.
    [27] GNIAZDOWSKA A, MIKULSKA M, RYCHTER A M. Growth, nitrate uptake and respiration rate in bean roots under phosphate deficiency[J]. Biologia Plantarum, 1998, 41(2): 217-226.
    [28] 张彦东,范志强,王庆成,等. 不同形态N素对水曲柳幼苗生长的影响[J]. 应用生态学报. 2000, 11(10): 665-667.
    [29] YUAN H, LIU D. Signaling components involved in plant responses to phosphate starvation[J]. Journal of Integrative Plant Biology, 2008, 50(7): 849-859.
    [30] ROTHSTEIN D E, CREGG B M. Effects of nitrogen form on nutrient uptake and physiology of Fraser fir( Abies fraseri )[J]. Forest Ecology and Management, 2005, 219: 69-80.
    [31] 张韫,崔晓阳. 不同N H 4 + /N O 3 - 配比作用下红松幼苗的氮吸收和同化特征[J]. 北京林业大学学报, 2011, 33(5): 61-64.
    [32] 郭延平,陈屏昭,张良诚,等. 不同供磷水平对温州蜜柑叶片光合作用的影响[J]. 植物营养与肥料学报, 2002, 8(2): 186-191.
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出版历程
  • 收稿日期:  2014-11-14
  • 修回日期:  2014-11-14
  • 刊出日期:  2015-08-31

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