3种滨藜属植物幼苗叶片对NaCl胁迫的生理响应

党晓宏; 高永; 蒙仲举; 包蕾; 王; 珊; 高君亮; 余新春; 王祯仪

doi:10.13332/j.1000-1522.20150393

3种滨藜属植物幼苗叶片对NaCl胁迫的生理响应

1.
1 内蒙古农业大学沙漠学院,中央与地方共建风沙物理重点实验室
2.
2 通辽市城乡规划技术管理中心
3.
3 中国林业科学研究院沙漠林业实验中心

基金项目:

“948”国家林业局引进项目(2015-4-22)、内蒙古自治区自然基金项目(2015MS0301)。

详细信息

作者简介:
党晓宏,讲师。主要研究方向:荒漠化防治。Email:dangxiaohong1986@126.com 地址:010010内蒙古呼和浩特市赛罕区鄂尔多斯东街29号内蒙古农业大学沙漠治理学院。
责任作者: 高永,教授,博士生导师。主要研究方向:荒漠化防治。Email:13948815709@163.com 地址:同上。

党晓宏,讲师。主要研究方向:荒漠化防治。Email:dangxiaohong1986@126.com 地址:010010内蒙古呼和浩特市赛罕区鄂尔多斯东街29号内蒙古农业大学沙漠治理学院。
责任作者: 高永,教授,博士生导师。主要研究方向:荒漠化防治。Email:13948815709@163.com 地址:同上。

计量
- 文章访问数: 2276
- HTML全文浏览量: 314
- PDF下载量: 25
出版历程
- 收稿日期: 2015-11-29
- 发布日期: 2016-10-28

Leaf physiological characteristics of seedlings of three Atriplex species under NaCl stress.

1.
1 Desert Science and Engineering College, Inner Mongolia Agriculture University, Wind Erosion Key Laboratory of Central and Local Government, Hohhot, Inner Mongolia, 010018, P.R. China;
2.
2 Urban and Rural Planning Management Center in Tongliao City, Tongliao,Inner Mongolia, 028000, P.R. China;
3.
3 Experimental Center of Desert Forestry, Chinese Academy of Forestry, Bayannur, Inner Mongolia, 015200, P.R. China.

摘要

摘要: 以灰白滨藜变种1、灰白滨藜变种2和四翅滨藜的1年生实生幼苗为试验材料,采用沙土盆栽试验,对不同质量浓度(0、0.1%、0.3%、0.5%、0.7%和1.0%)NaCl单盐胁迫条件下3种滨藜属植物幼苗叶片盐害指数、耐盐阈值及生理特性等指标进行了测定。结果表明:盐胁迫提高了3种滨藜属植物幼苗各器官Na+浓度,降低了K+浓度,灰白滨藜变种2较其他2种植物各器官保持了相对较低的Na+和较高K+浓度,有利于维持较高的K+/Na+平衡;随着土壤盐浓度的增加,灰白滨藜变种2叶片受盐害起始浓度、耐盐阈值及耐盐极限浓度均高于灰白滨藜变种1和四翅滨藜,最终确定出灰白滨藜变种1、灰白滨藜变种2和四翅滨藜的耐盐阈值分别为0.59%、0.68%和0.63%;叶片丙二醛含量、细胞膜相对透性随着盐胁迫程度的加剧而增加。经隶属函数综合评价认为3种滨藜属植物耐盐性强弱顺序依次为:灰白滨藜变种2(0.686)＞四翅滨藜(0.532)＞灰白滨藜变种1(0.102);与灰白滨藜变种1相比,灰白滨藜变种2和四翅滨藜各项生理指标特征对盐土环境的适应能力更强。
- 耐盐性 /
- NaCl胁迫 /
- 苗期 /
- 生理响应 /
- 滨藜属植物
Abstract: Atriplex species, such as Atriplex canescens ssp. aptera, A. canescens ssp. canescens var. laciniata and A. canescens, as unique quasi-evergreen shrubs in arid and semi-arid desert area, are of high socio-ecological importance in controlling desertification, improving soil quality in saline land, and supporting local ecological balance. Here we measured salt injury index, salt-tolerant thresholds and physiological characteristics of the first-year seedlings by a salt-stress experiment conducted in pots. In the experiment, the pots were filled with sand and the six salt levels were adjusted to 0, 0.1%, 0.3%, 0.5%, 0.7%, and 1.0% NaCl. The results showed that: 1) Na+ increased and K+ decreased in each part of three Atriplex seedlings under salt stress. Compared with A. canescens ssp. aptera and A. canescens, the relatively lower Na+ and higher K+ contents were retained in each part of A. canescens ssp. canescens var. laciniata, which was conducive to keeping higher K+/Na+ balance. 2) The initial NaCl concentration with salt stress, salt-tolerance threshold and salt concentration limit of A. canescens ssp. canescens var. laciniata were higher than the other two plants. 3) The salt-tolerance thresholds of A. canescens ssp. aptera, A. canescens ssp. canescens var. laciniata and A. canescens were 0.59%, 0.68%, and 0.63%, respectively. 4) With salt stress increasing, MDA content and cytomembrane permeability were consistently increasing. 5) The salt-tolerance thresholds of the three species based on subordinate function comprehensive evaluation were in the order of A. canescens ssp. canescens var. laciniata (0.686)＞A. canescens (0.532)＞A. canescens ssp. aptera (0.102). It is suggested that A. canescens ssp. canescens var. laciniata has a stronger adaptability to saline soil than A. canescens and A. canescens ssp. aptera.
- salt tolerance /
- NaCl stress /
- seedling stage /
- physiological response /
- Atriplex species

HTML全文

参考文献(84)

[1]	谢振宇,杨光穗.牧草耐盐性研究进展[J].草业科学,2003,20(8):11-15.
[1]	XIE Z Y, YANG G S.Advances in salt tolerance of forage plants[J].Pratacultural Science,2003,20(8):11-15.
[2]	LIAO Y, PENG Y G, CHEN G Z.Research advances in plant salttolerance mechanism[J]. Acta Ecologica Sinica, 2007,27(5):2077-2089.
[2]	廖岩,彭友贵,陈桂珠.植物耐盐性机理研究进展[J].生态学报,2007,27(5):2077-2089.
[3]	肖强,郑海雷,陈瑶,等.盐度对互花米草生长及脯氨酸、可溶性糖和蛋白质含量的影响[J].生态学杂志,2005,24(4):373-376.
[3]	XIAO Q, ZHENG H L,CHEN Y,et al. Effects of salinity on the growth and proline, soluble sugar and protein contents of Spartina alterniflora[J]. Chinese Journal of Ecology,2005,24(4):373-376.
[4]	GUO H J,HU T,FU J M.Effects of saline sodic stress on growth and physiological responses of Lolium perenn[J].Acta Prataculturae Sinica,2012,21(1):118-125.
[4]	郭慧娟,胡涛,傅金民.苏打碱胁迫对多年生黑麦草的生理影响[J].草业学报,2012,21(1):118-125.
[5]	FAN R P, ZHOU Q,ZHOU B,et al. Effects of salinization stress on growth and the antioxidant system of tall fescue[J]. Acta Prataculturae Sinica,2012,21(1):112-117.
[5]	樊瑞苹,周琴,周波,等.盐胁迫对高羊茅生长及抗氧化系统的影响[J].草业学报,2012,21(1):112-117.
[6]	LIU Z X, ZHANG H X,YANG S,et al. Effects of NaCl stress on growth and photosynthetic characteristics of Elaeagnus angustifolia seedlings[J].Scientia Silvae Sinicae,2014,50(1):32-40.
[6]	刘正祥,张华新,杨升,等.NaCl 胁迫对沙枣幼苗生长和光合特性的影响[J].林业科学,2014,50(1):32-40.
[7]	WANG L J,MA L Y,WANG S, et al.Effects of water and salt stress on chlorophyll fluorescence parameters and pigment contents of Elaeagnus angustifolia L. seedlings[J]. Acta Agriculturae BorealiOccidentalis Sinica, 2010,19(12): 122-127.
[7]	王利军,马履一,王爽,等.水盐胁迫对沙枣幼苗叶绿素荧光参数和色素含量的影响[J].西北农业学报, 2010,19(12): 122-127.
[8]	LIU J X,WANG X,LI B P. Effects of exogenous nitric oxide donor on photosynthesis and xanthophyll cycle of Peganum multisectum seedlings under NaCl stress[J]. Journal of Desert Research,2011,31(1):137-141.
[8]	刘建新,王鑫,李博萍.外源一氧化氮供体对盐胁迫下多裂骆驼蓬幼苗光合作用和叶黄素循环的影响[J].中国沙漠,2011,31(1):137-141.
[9]	ZHANG R H, LI J, GUO S R, et al. Effects of exogenous putrescine on gasexchange characteristics and chlorophyll fluorescence of NaClstressed cucumber seedlings [J]. Photosynth Res, 2009, 100:155-162.
[10]	WANG P, YIN L J,LI J D. Studies of stresses of neutral land alkaiine salts on Aneurolepidium chinense seedling[J]. Acta Prataculturae Sinica, 1994,3(2):37-43.
[10]	王萍,殷丽娟,李建东.中性盐和碱性盐对羊草幼苗胁迫的研究[J].草业学报,1994,3(2):37-43.
[11]	LI J. Research on physiological and biochemical indices of drought resistance of four species in Atriplex[D].Lanzhou:Gansu Agricultural University,2006.
[11]	李健.滨藜属4种植物抗旱性生理生化指标研究 [D].兰州:甘肃农业大学,2006.
[12]	DAYTON W A. Range plant handbook [M]. Washington: Forest Service, U S Gov Printing Office, 1937.
[13]	李梅. 征服沙尘暴的神奇植物:美国滨藜[J].农业环境与发展,2002(4):19.
[13]	LI M.The magical plant of coquetting dust storm: American Atriplex L[J］. Agroenvirenment and Development, 2002(4):19.
[14]	RUMBAUGH M D. Forage yield and quality in a Great Basin shrub, grass and legume pasture experiment[J］. Range Manage, 1982, 35: 604-609.
[15]	ZHANG S T.American Atriplex canescens (Pursh) Nutt[J].Hebei Agriculture Science, 2003(2):16.
[15]	张树桐.美国四翅滨藜[J].河北农业科技,2003(2):16.
[16]	王文颖,王刚.四翅滨藜的生物:生态学特性及研究进展[J].草业科学,2004,21(7):18-20.
[16]	WANG W Y, WANG G. Bioecological characteristic and research progress for Atriplex canescens [J].Pratacultural Science,2004,21(7):18-20.
[17]	王存桂.四翅滨藜推广现状和发展建议[J].甘肃科技, 2002,3(7):97-98.
[17]	WANG C G. Extension present situation and developmental advise of Atriplex canescens [J].Gansu Science and Technology, 2002,3(7):97-98.
[18]	刘炳响,王志刚,梁海永,等.盐胁迫对不同生境白榆生理特征与耐盐性的影响[J].应用生态学报,2012,23(6):1481-1489.
[18]	LIU B X, WANG Z G,LIANG H Y,et al. Effects of salt stress on physiological characters and salttolerance of Ulmus pumila in different habitats[J].Chinese Journal of Applied Ecology,2012,23(6):1481-1489.
[19]	ZHAO M F,GE C,ZHAI Z Z. Study on the determination of salttolerance index of main afforestation tree species and their ordination in arid areas with secondary salinization[J].Forestry Research,1997,15(2):256-258.
[20]	赵明范,葛成,翟志中.干旱地区次生盐碱地主要造林树种抗盐指标的确定及耐盐能力排序[J].林业科学研究,1997,15(2):256-258.
[20]	ZOU Q.Plant physiological experimental guide[M].Beijing:China Agricultural Press,2000:3-4.
[21]	邹琦.植物生理学实验指导[M].北京:中国农业出版社, 2000:3-4.
[21]	LI L X, LIANG Z S, HAN R L. Effect of soil drought on the growth and water use efficiency of seabuckthorn[J].Acta Bot Boreal Occident Sin,2002,12(5):121-124.
[22]	JIAO L L,LU B S,BAI Z Y,et al. The comparation of cold resistance of four gardening young trees [J].Acta Horticulturaae Sinica,2006,33(3): 667-670.
[22]	李丽霞,梁宗锁,韩蕊莲.土壤干旱对沙棘苗木生长及水分利用的影响[J].西北植物学报,2002,12(5):121-124.
[23]	National Agricultural Technology Extension Service Center. Soil analysis technical specification[M].Beijing: China Agricultural Press,2006:93-112.
[23]	缴丽莉,路丙社,白志英,等.四种园林树木抗寒性的比较分析[J].园艺学报,2006,33(3): 667-670.
[24]	全国农业技术推广服务中心.土壤分析技术规范[M].北京:中国农业出版社,2006：93-112.
[24]	ZHOU G S,MEI F Z,ZHOU Z Q, et al. Comprehensive evaluation and forecast on physiological indices of waterlogging resistance of different wheat varieties[J].Scientia Agricultura Sinica,2003,36(11):1378-1382.
[25]	周广生,梅方竹,周竹青,等.小麦不同品种耐湿性生理指标综合评价及其预测[J].中国农业科学,2003,36(11):1378-1382.
[25]	ZHANG C Y, XU G F. Comprehensive evaluation of heat tolerance of four ground covering plants by subordinate function values analysis [J]. Pratacultural Science, 2009,26 (2):57-60.
[26]	WANG R G, CHEN S L, ZHOU X Y, et al. Ionic homeostasis and reactive oxygen species control in leaves andxylem sap of two poplars subjected to NaCl stress[J]. Tree Physiology, 2008, 28: 947-957.
[26]	张朝阳, 许桂芳.利用隶属函数法对4种地被植物的耐热性综合评价[J].草业科学,2009,26 (2):57- 60.
[27]	DING M Q, HOU P C, SHEN X, et al. Saltinduced expression of genes related to Na+/K+ and ROS homeostasis in leaves of saltresistant and saltsensitive poplar species[J]. Plant Molecular Biology, 2010, 73: 251-269.
[28]	TEAKLEA N L, BAZIHIZINAA N,SHABALAC S, et al. Ion transport in halophytes[J]. Advances in Botanical Research, 2011, 57: 151-199.
[29]	YANG X C, LI W J, QU Y, et al. Impact on salt stress on growth and ion uptake of different parts of oil sunflower seedlings[J].Agricultural Science and Technology,2011,12(3):354-358.
[30]	ZHAO K F, FAN H.Halophytes and its adapt physiology to saline habitat[M].Beijing:Science Press,2005:14-76,48-51.
[31]	赵可夫,范海.盐生植物及其对盐渍生境的适应生理[M].北京:科学出版社,2005:14-76,48-51.
[31]	TAKAHASHI R, NISHIO T, ICHIZEN N, et al. Cloning and functional analysis of the K+transporter, PhaHAK2, from saltsensitive and salttolerant reed plants[J]. Biotechnology Letters, 2007, 29: 501-506.
[32]	LIU Z Q, ZHANG S C. Plant resistance physiology[M].Beijing: China Agricultural Press,1994:84-90.
[33]	刘祖褀,张石城.植物抗性生理学[M].北京:中国农业出版社,1994:84-90.
[33]	WANG N,YANG J,HUANG Q,et al. Physiological salinity tolerance mechanism for transport of K+and Na+ions in cotton (Gossypium hirsutum L.) seedlings under salt stress[J]. Cotton Science,2015,27(3):208-215.
[34]	CI D W,ZHANG Z M,DING H,et al. Evaluation and selection indices of salinity tolerance in peanut seedling [J]. Acta Ecologica Sinica, 2015,35(3):805-814.
[34]	王宁,杨杰,黄群,等.盐胁迫下棉花K+和Na+离子转运的耐盐性生理机制[J].棉花学报,2015,27(3):208-215.
[35]	YU Z,SUN X,ZHANG W Z,et al. Studies on the differences of salt tolerance between seed germination and seedling of alfalfa cultivars[J]. Journal of Arid Land Resources and Environment,1993,7(2):106-111.
[35]	慈敦伟,张智猛,丁红,等.花生苗期耐盐性评价及耐盐指标筛选[J].生态学报,2015,35(3):805-814.
[36]	于卓,孙祥,张文忠,等.苜蓿品种间种子萌发及苗期耐盐性差异的研究[J].干旱区资源与环境,1993,7(2):106-111.
[36]	LU Y,LEI J Q,ZENG F J,et al.Effects of salt stress on Nitraria roborowskii growth and physiological characteristics of stress resistance[J].Chinese Journal of Applied Ecology,2014,25(3):711-717.
[37]	CHEN G L,WANG C X,CHEN J Y. Osmosisregulating substance and cation contents in Nitraria sibirica Pall. testtube plantlets with NaCl stress [J]. Acta Bot Boreal Occident Sin, 2009,29(6): 1233-1239.
[37]	鲁艳,雷加强,曾凡江,等.NaCl 胁迫对大果白刺幼苗生长和抗逆生理特性的影响[J].应用生态学报, 2014,25(3):711-717.
[38]	陈贵林,王晨霞,陈建英.NaCl胁迫对白刺试管苗渗透调节物质及离子含量的影响[J].西北植物学报,2009,29(6): 1233-1239.
[38]	YAN X X,ZHAO T F,HU Y J. Effect of salt stress on cells in root tips of barley[J]. Acta Agriculturae BorealiSinica,1994, 9(Suppl.): 61-64.
[39]	MAO P C,TIAN X X,MENG L. Evaluation of salt tolerance for 16 Iris lacteal var. chinensis accessions at seedling stage[J]. Pratacultural Science,2013,30(1):35-43.
[39]	闫先喜,赵檀方,胡延吉.中度盐胁迫对大麦根尖细胞的影响[J]. 华北农学报，1994, 9(增刊): 61-64.
[40]	毛培春,田小霞,孟林. 16份马蔺种质材料苗期耐盐性评价[J].草业科学,2013,30(1):35-43.
[40]	ZHANG H X,SONG D,LIU Z X.Study on physiological characteristics and salt tolerance for seedlings of 11 tree species[J]. Forest Research,2008,21(2):168-175.
[41]	LI X F,NI Z M,WU Y Y,et al. Effects of salt stress on photosynthetic characteristics and leaf cell structure of ‘Yinhong’ grape seedlings[J].Acta Ecologica Sinica,2015,35(13): 4436-4444.
[41]	张华新,宋丹,刘正祥.盐胁迫下11个树种生理特性及其耐盐性研究[J].林业科学研究,2008,21(2):168-175.
[42]	ZHANG Z M,CI D W,DING H,et al. Indices selection and comprehensive evaluation of salinity tolerance for peanut varieties[J]. Chinese Journal of Applied Ecology, 2013,24(12):3487-3494.
[42]	李学孚,倪智敏,吴月燕,等.盐胁迫对“鄞红”葡萄光合特性及叶片细胞结构的影响[J].生态学报,2015,35(13): 4436-4444.
[43]	张智猛,慈敦伟,丁红,等.花生品种耐盐性指标筛选与综合评价[J].应用生态学报, 2013,24(12):3487-3494.
[43]	DONG Z G,CHENG Z H. Salt tolerance and assessment of salt tolerance indices of tomato varieties in sprout stage and seedling stage [J]. Acta Ecologica Sinica,2009,29(3): 1348-1355.
[44]	董志刚,程智慧.番茄品种资源芽苗期和幼苗期的耐盐性及耐盐指标评价[J].生态学报,2009,29(3): 1348-1355.
[44]	WANG B S,ZOU Q,ZHAO K F. Response of different organ growth of sorghum to NaCl stress and the threshold salinity [J]. Acta Bot Boreal Occident Sin, 1997, 17(3):279-285.
[45]	ZHANG H X,LIU Z X,LIU Q F. Seedling growth and salt tolerance of tree species under NaCl stress [J]. Acta Ecologica Sinica,2009,29(5):2263-2271.
[45]	王宝山,邹绮,赵可夫.高粱不同器官生长对NaCl 胁迫的响应及耐盐阈值[J].西北植物学报, 1997,17(3):279-285.
[46]	SU X H, QIANG S,SONG X L. Comparison of Eupatorium adenophorum heat tolerances of different geographical populations [J]. Acta Bot Boreal Occident Sin, 2005,25(9): 1766-1771.
[46]	张华新,刘正祥,刘秋芳.盐胁迫下树种幼苗生长季耐盐性[J］.生态学报,2009,29(5):2263-2271.
[47]	苏秀红,强胜,宋小玲.不同地理种群紫茎泽兰耐热性差异的比较分析[J].西北植物学报,2005,25( 9) : 1766-1771.

施引文献(16)

期刊类型引用(11)

1.	赵熙来，周正，葛锐，罗伟豪，马旭彤，蒋慧，苏华维. 残次香梨与乳酸菌组合对四翅滨藜青贮的影响. 中国饲料. 2024(17): 155-161 . 百度学术
2.	张衡锋，杨绮，韦庆翠，张焕朝. 盐胁迫对10个品种紫薇的影响及其耐盐性综合评价. 东北林业大学学报. 2023(09): 34-40 . 百度学术
3.	李雨欣，罗秀丽，张婷婷，康宇乾，王鹏，江行玉，周扬. 盐胁迫下海马齿生理指标变化及相关基因表达分析. 农业生物技术学报. 2022(07): 1279-1289 . 百度学术
4.	刘鹤莹，张嫚，翟中葳，杨鹏，支苏丽，沈仕洲，张克强. 大薸对奶厅废水主要污染物的去除效果研究. 农业环境科学学报. 2022(11): 2525-2538 . 百度学术
5.	王涛，蒙仲举，张佳鹏，雷虹娟，张格. NaCl胁迫对紫穗槐幼苗生长及生理特性的影响. 西北林学院学报. 2021(01): 25-30 . 百度学术
6.	刘学良，姚俊修，刘翠兰，李善文，任飞，李庆华，吴海涛，翟红莲，吴德军，邢世岩，高红萍. 7个接骨木无性系苗木对盐胁迫的生理响应与评价. 中南林业科技大学学报. 2021(01): 37-44+79 . 百度学术
7.	鲁俊倩，武舒，钟姗辰，张伟溪，苏晓华，张冰玉. ‘84K’杨组氨酸激酶基因PaHK3a的表达及功能分析. 北京林业大学学报. 2021(02): 46-53 . 本站查看
8.	丁丁，王红宝，郑伶杰，左永梅，韩民利，吴新海，郭艳超. 不同品种茶菊对NaCl胁迫的生理响应及耐盐性评价. 植物生理学报. 2021(03): 692-702 . 百度学术
9.	赵佳伟，李清亚，路斌，李艳，朱玉菲，栗浩，路丙社. 不同品种北美豆梨对NaCl胁迫的生理响应及耐盐性评价. 植物生理学报. 2019(01): 23-31 . 百度学术
10.	邹晓君，列志旸，薛立. NaCl胁迫对4种园林植物养分含量和贮量的影响. 华南农业大学学报. 2018(06): 77-84 . 百度学术
11.	杨传宝，孙超，李善文，姚俊修，刘敬国，矫兴杰. 白杨派无性系苗期耐盐性综合评价及筛选. 北京林业大学学报. 2017(10): 24-32 . 本站查看

其他类型引用(5)

资源附件(0)

计量

文章访问数: 2276
HTML全文浏览量: 314
PDF下载量: 25
被引次数: 16

3种滨藜属植物幼苗叶片对NaCl胁迫的生理响应

计量

出版历程

Leaf physiological characteristics of seedlings of three Atriplex species under NaCl stress.

期刊类型引用(11)

其他类型引用(5)

计量

出版历程

目录