Combined ABTS radical scavenging activity of Pinus koraiensis polyphenols with fungus polysaccharides.
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摘要: 本文比较了红松多酚(PKP)与黑木耳多糖(AAP)和灵芝孢子多糖(GLP)单一、联合使用清除ABTS自由基活性的作用。采用Chou-Talaly联合指数方法分析ABTS自由基清除实验结果,并对单一和联合使用的清除效果、联合指数(CI)、等效应图和剂量减少指数(DRI)进行分析评价。结果显示:单独使用红松多酚、黑木耳多糖、灵芝孢子多糖的IC50值分别为72.01 μg/mL、2.49 mg/mL、0.93 mg/mL,联合使用红松多酚和黑木耳多糖与红松多酚和灵芝孢子多糖(质量比为1∶3)后,IC50值分别为185.20和237.83 μg/mL。联合指数分析表明,从清除率10%到97%,红松多酚和黑木耳多糖的联合指数都小于1;等效应图显示联合使用红松多酚和黑木耳多糖使用剂量降低。剂量减少指数分析显示,从清除率5%到97%,红松多酚和黑木耳多糖的剂量减少指数都大于1,以上结果红松多酚和灵芝孢子多糖均表现不一致。说明红松多酚和黑木耳多糖之间存在协同清除ABTS自由基效应,但红松多酚和灵芝孢子多糖不存在。Abstract: We compared the ABTS radical scavenging activity of Pinus korsiensis polyphenols (PKP) alone or combined with Auricularia auricular polysaccharides (AAP) and Ganoderma lucidum spore polysaccharides (GLP). ABTS radical scavenging experiments and Chou-Talalay combination index (CI) method were used to evaluate the scavenging effects of PKP with AAP and GLP individually as well as in combination, and combination index (CI), isobologram and dose-reduction index (DRI) were worked out subsequently. It showed that IC50 values of PKP, AAP and GLP were 72.01 μg/mL, 2.49 mg/mL and 0.93 mg/mL, respectively, while IC50 values of the combination (mass ratio 1∶3) were 185.20 and 237.83 μg/mL, respectively. CI analysis indicated that the clearance rate was 10%-97%, and CI of PKP with AAP was less than 1. Dose of PKP with AAP was reduced based on isobologram. DRI analysis demonstrated that the clearance rate was 5%-97%, and DRI of PKP with AAP was greater than 1. The results of PKP with GLP were inconsistent. Our study suggests that the combination of PKP and AAP has significantly improved the ABTS radical scavenging activity, compared with using them individually.
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