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基于BWB的油松初级种子园混合子代优树选择与配置设计

袁虎威 王晓飞 杜清平 钮世辉 李悦 李伟

袁虎威, 王晓飞, 杜清平, 钮世辉, 李悦, 李伟. 基于BWB的油松初级种子园混合子代优树选择与配置设计[J]. 北京林业大学学报, 2017, 39(11): 28-34. doi: 10.13332/j.1000-1522.20160330
引用本文: 袁虎威, 王晓飞, 杜清平, 钮世辉, 李悦, 李伟. 基于BWB的油松初级种子园混合子代优树选择与配置设计[J]. 北京林业大学学报, 2017, 39(11): 28-34. doi: 10.13332/j.1000-1522.20160330
YUAN Hu-wei, WANG Xiao-fei, DU Qing-ping, NIU Shi-hui, LI Yue, LI Wei. BWB-assisted plus tree selection and deployment design for bulked progenies of the first-cycle Chinese pine seed orchard[J]. Journal of Beijing Forestry University, 2017, 39(11): 28-34. doi: 10.13332/j.1000-1522.20160330
Citation: YUAN Hu-wei, WANG Xiao-fei, DU Qing-ping, NIU Shi-hui, LI Yue, LI Wei. BWB-assisted plus tree selection and deployment design for bulked progenies of the first-cycle Chinese pine seed orchard[J]. Journal of Beijing Forestry University, 2017, 39(11): 28-34. doi: 10.13332/j.1000-1522.20160330

基于BWB的油松初级种子园混合子代优树选择与配置设计

doi: 10.13332/j.1000-1522.20160330
基金项目: 

国家自然科学基金项目 31600535

北京市自然科学基金项目 5164033

林业公益性行业科研专项 201104022

详细信息
    作者简介:

    袁虎威,博士,讲师。主要研究方向:林木遗传改良、经济林嫁接分子机制。Email: yuanhuwei86@163.com  地址: 311300 浙江省杭州市临安市环城北路88号浙江农林大学林业与生物技术学院

    责任作者:

    李悦,博士,教授。主要研究方向:森林遗传学与针叶树遗传改良。Email: liyue@bjfu.edu.cn  地址:同上

    李伟,博士,教授。主要研究方向:针叶树遗传改良。Email: bjfuliwei@bjfu.edu.cn   地址:同上

  • 中图分类号: S722.8+3

BWB-assisted plus tree selection and deployment design for bulked progenies of the first-cycle Chinese pine seed orchard

  • 摘要: 当前甘肃中湾油松良种基地正处于由第一育种周期向第二育种周期发展的关键转折时期,如何以前期改良成果为基础,结合现代林木育种的先进理论和技术,更高效地筛选和利用第二育种周期种质资源已经成为亟待解决的重要问题。本研究以甘肃中湾油松良种基地大规模初级种子园无谱系自由授粉混合子代群体为研究对象,详细阐述了基于种子园自由授粉混合子代的油松高育种周期优良种质资源选择、评价及配置利用策略等问题。结果表明,按照单株材积优势比的相对大小从中湾自由授粉混合子代中选出40个优良单株,其平均材积优势比为0.83;优良单株群体具有较高的遗传多样性,其平均多态性信息含量为0.620 2。基于SSR分子标记的亲缘分析结果表明,40个单株间亲缘关系不同,分属于23个同源组;为有效控制近交,进行了同源组内优良单株的二次选择,最终入选23个优良单株,并将之用作第二育种周期种子园的建园亲本。结合建园亲本材积优势比和亲缘分析的结果,制定了中湾第二育种周期种子园的分组、不平衡、完全、固定区组配置设计策略,兼顾了种子园子代材积遗传增益的提高和遗传多样性的维持。研究结果为油松高育种周期优良种质资源的高效选择及合理利用提供了新的思路,为缩短油松育种周期、提高油松育种效率奠定了基础。

     

  • 图  1  油松第二育种周期优良单株的材积优势比

    Figure  1.  Dominance ratios of volume for superior individuals in the second breeding cycle of Chinese pine

    图  2  第二育种周期油松优良单株的分子指纹图谱(a)及相对亲缘关系(b)

    Figure  2.  Molecular fingerprints (a) and relative phylogenetic relationships (b) of superior individuals in the second breeding cycle of P. tabuliformis

    图  3  油松第二育种周期优良单株群体的遗传多样性

    Figure  3.  Genetic diversity of superior individual population in the second breeding cycle of Chinese pine

    图  4  油松第二育种周期种子园配置设计

    Figure  4.  Deployment design of clones in the second breeding cycle seed orchard of Chinese pine

    表  1  油松第二育种周期种子园亲缘组和配置组分组情况

    Table  1.   Phylogenetic group and deployment group in the second breeding cycle seed orchard of Chinese pine

    组别
    Group
    无性系
    编号
    Clone No.
    无性系
    编号
    Clone No.
    无性系
    编号
    Clone No.
    无性系
    编号
    Clone No.
    无性系
    编号
    Clone No.
    1 6 25 36 20
    2 15 29
    3 1 33 37 7
    4 27 22 38 13 17
    5 26 21 23
    6 2 24 40 30 31
    下载: 导出CSV
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出版历程
  • 收稿日期:  2016-10-10
  • 修回日期:  2017-04-14
  • 刊出日期:  2017-11-01

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