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草酸和柠檬酸提高长白落叶松对Pb胁迫的适应性

宋金凤 李金博 曹楷 桑英 崔晓阳

宋金凤, 李金博, 曹楷, 桑英, 崔晓阳. 草酸和柠檬酸提高长白落叶松对Pb胁迫的适应性[J]. 北京林业大学学报, 2017, 39(11): 18-27. doi: 10.13332/j.1000-1522.20170146
引用本文: 宋金凤, 李金博, 曹楷, 桑英, 崔晓阳. 草酸和柠檬酸提高长白落叶松对Pb胁迫的适应性[J]. 北京林业大学学报, 2017, 39(11): 18-27. doi: 10.13332/j.1000-1522.20170146
SONG Jin-feng, LI Jin-bo, CAO Kai, SANG Ying, CUI CUI Xiao-yang. Oxalic and citric acids enhancing adaptation of Changbai Larix olgensis to soil Pb stress[J]. Journal of Beijing Forestry University, 2017, 39(11): 18-27. doi: 10.13332/j.1000-1522.20170146
Citation: SONG Jin-feng, LI Jin-bo, CAO Kai, SANG Ying, CUI CUI Xiao-yang. Oxalic and citric acids enhancing adaptation of Changbai Larix olgensis to soil Pb stress[J]. Journal of Beijing Forestry University, 2017, 39(11): 18-27. doi: 10.13332/j.1000-1522.20170146

草酸和柠檬酸提高长白落叶松对Pb胁迫的适应性

doi: 10.13332/j.1000-1522.20170146
基金项目: 

国家自然科学基金项目 31370613

中央高校基本科研业务费专项 2572017CA03

详细信息
    作者简介:

    宋金凤,博士,副教授。主要研究方向:森林土壤学与土壤生态学。Email:songnefu@126.com   地址:150040 黑龙江省哈尔滨市东北林业大学林学院

    责任作者:

    崔晓阳,教授,博士生导师。主要研究方向:森林土壤学与土壤生态学。Email:c_xiaoyang@126.com   地址:同上

  • 中图分类号: Q945.78; S718.43

Oxalic and citric acids enhancing adaptation of Changbai Larix olgensis to soil Pb stress

  • 摘要: 通过外源添加不同浓度草酸或柠檬酸溶液,系统研究了不同处理时间下,草酸和柠檬酸对土壤Pb胁迫下长白落叶松幼苗多种生理生化特性、元素吸收及生长的影响,特别是叶绿素荧光参数、根系形态特征及养分元素吸收状况。结果表明,Pb胁迫对长白落叶松产生毒害,叶片细胞膜透性和F0提高,叶片可溶性蛋白含量、叶绿素荧光参数(FmFvFv/FmFv/F0比率)、根系表面积、长度、体积和比根长均下降。细根和叶片K、Ca、Fe、Pb含量及细根Mg含量显著增加,但叶片Mg含量降低,根、茎和叶干质量也显著降低。时间越长Pb对苗木的毒害越重。外源草酸和柠檬酸处理使上述生理生化特性和根系形态指标均向相反方向变化,并显著提高根、茎和叶等各部分干质量,提高了苗木对Pb胁迫的抵抗力和适应性。有机酸对上述指标的影响一般在20或30 d,5.0或10.0 mmol/L效果最佳,且柠檬酸效果强于草酸。草酸和柠檬酸降低苗木细根和叶片Mg、K、Ca含量, 但增加Fe含量。较低浓度有机酸处理时,苗木细根和叶片Pb含量降低,此时苗木对Pb胁迫的解毒机制主要体现在外部排斥机制,而较高浓度时(5.0~10.0 mmol/L)Pb含量却升高,此时内部耐受机制发挥的作用更大,但也不排除2种机制同时起作用的可能性。研究能为有机酸应用于植物修复土壤Pb胁迫提供理论指导,也能为东北林区Pb胁迫土壤的有效利用开辟新思路。

     

  • 图  1  有机酸处理后长白落叶松叶片的相对电导率

    不同字母表示差异显著(P < 0.05)。

    Figure  1.  Relative conductivity of Larix olgensis leaves with different organic acids

    Bars labeled with different letters are significantly different (P < 0.05).

    图  2  有机酸处理后长白落叶松叶片的可溶性蛋白含量

    Figure  2.  Soluble protein contents of Larix olgensis leaves with different organic acid treatments

    图  3  有机酸处理后长白落叶松叶片的叶绿素荧光参数

    Figure  3.  Chlorophyll fluorescence parameters of Larix olgensis leaves induced by different organic acids

    图  4  有机酸处理后长白落叶松幼苗的根系形态特征

    Figure  4.  Root morphological parameters of Larix olgensis seedlings induced by different organic acids

    图  5  有机酸处理后长白落叶松细根和叶片Pb及营养元素含量

    Figure  5.  Pb and nutrient contents in fine roots and leaves of Larix olgensis seedlings with organic acid treatments

    图  6  有机酸处理后长白落叶松的根、茎和叶干质量

    △ OA叶OA leaf ▲ CA叶CA leaf ○ OA茎OA stem ● CA茎CA stem □ OA根OA root ■ CA根CA root

    Figure  6.  Dry mass of root, stem and leaf of Larix olgensis seedlings with organic acid treatments

    表  1  供试A1层暗棕壤的基本性质

    Table  1.   Selected properties of A1 horizon of dark brown forest soil used in the experiment

    土壤层次
    Soil layer
    pH值
    pH value (H2O)
    阳离子交换量
    Cation exchange capacity/(cmol·kg-1)
    有机质
    Organic matter/ (g·kg-1)
    全氮
    Total N/ (g·kg-1)
    全磷
    Total P/ (g·kg-1)
    速效磷
    Available P/ (mg·kg-1)
    土壤质地
    Soil texture
    黏粒Clay (<2 μm)/ (g·kg-1) 草酸含量
    Oxalic acid concentration/(μg·kg-1)
    柠檬酸含量
    Citric acid concentration/(μg·kg-1)
    A1 5.32 40.2 107.9 6.39 2.08 45.8 壤质
    Loamy texture
    135 310.98 139.83
    注:30 mmol/L NH4F+25 mmol/L HCl浸提法;A1为暗棕壤层。下同。Notes: means extracted with 30 mmol/L NH4F+25 mmol/L HCl; A1 means horizon of dark brown forest soil. The same below.
    下载: 导出CSV

    表  2  实验设计(10、20、30 d均进行如下处理)

    Table  2.   Experimental layout (each of the treatments below was sampled after 10, 20, or 30 days)

    处理Treatment 土壤处理Soil treatment 有机酸类型Kind of organic acid 有机酸浓度Concentration of organic acid/(mmol·L-1)
    CK A1、无Pb
    A1, no Pb
    0
    T1 A1+Pb 0
    T2 A1+Pb OA 0.2
    T3 A1+Pb OA 1.0
    T4 A1+Pb OA 5.0
    T5 A1+Pb OA 10.0
    T6 A1+Pb CA 0.2
    T7 A1+Pb CA 1.0
    T8 A1+Pb CA 5.0
    T9 A1+Pb CA 10.0
    下载: 导出CSV
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  • 收稿日期:  2017-05-17
  • 修回日期:  2017-07-14
  • 刊出日期:  2017-11-01

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