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锌胁迫对银中杨生长发育和化学防御的影响

姜礅 王月月 严善春

姜礅, 王月月, 严善春. 锌胁迫对银中杨生长发育和化学防御的影响[J]. 北京林业大学学报, 2018, 40(11): 42-48. doi: 10.13332/j.1000-1522.20180131
引用本文: 姜礅, 王月月, 严善春. 锌胁迫对银中杨生长发育和化学防御的影响[J]. 北京林业大学学报, 2018, 40(11): 42-48. doi: 10.13332/j.1000-1522.20180131
Jiang Dun, Wang Yueyue, Yan Shanchun. Effects of Zn stress on growth development and chemical defense of Populus alba 'berolinensis' seedlings[J]. Journal of Beijing Forestry University, 2018, 40(11): 42-48. doi: 10.13332/j.1000-1522.20180131
Citation: Jiang Dun, Wang Yueyue, Yan Shanchun. Effects of Zn stress on growth development and chemical defense of Populus alba 'berolinensis' seedlings[J]. Journal of Beijing Forestry University, 2018, 40(11): 42-48. doi: 10.13332/j.1000-1522.20180131

锌胁迫对银中杨生长发育和化学防御的影响

doi: 10.13332/j.1000-1522.20180131
基金项目: 

东北林业大学学术名师支持计划项目 010602071

详细信息
    作者简介:

    姜礅,博士生。主要研究方向:昆虫化学生态。Email: 821880041@qq.com  地址:150040 黑龙江省哈尔滨市和兴路26号东北林业大学林学院

    责任作者:

    严善春,教授,博士生导师。主要研究方向:昆虫化学生态。Email: yanshanchun@126.com  地址:同上

  • 中图分类号: S718.43;S792.119

Effects of Zn stress on growth development and chemical defense of Populus alba 'berolinensis' seedlings

  • 摘要: 目的探明Zn胁迫对银中杨生长发育的影响及银中杨化学防御对Zn胁迫的响应趋势。方法本研究用3种不同含量的Zn(300、500、700 mg/kg)处理盆栽1年生银中杨的土壤,分析其生长发育状况、叶片内营养物质和次生物质含量以及蛋白酶抑制剂活性。结果Zn各含量胁迫使银中杨的生长参数(株高、根长和地径)和生物量参数(根、茎、叶的鲜质量和干质量)均显著低于对照(P<0.05),且具有一定的浓度效应。在Zn胁迫后30、40和50 d,各含量处理均使银中杨叶片中的蛋白质和可溶性糖含量显著低于对照(P<0.05),但处理组之间的蛋白质含量差异均不显著(P>0.05),而可溶性糖含量均表现为随Zn胁迫含量的增高而增高。Zn各含量胁迫均使银中杨叶片内的黄酮和木质素含量在各时间段显著高于对照,而叶片内的胰蛋白酶抑制剂(TI)和胰凝乳蛋白酶抑制剂(CI)活性却均显著低于对照(P<0.05)。结论生长基质中过量的Zn会抑制银中杨的生长发育,且能影响银中杨的化学防御水平。银中杨叶片内营养物质含量、抗虫相关次生代谢物质的含量、以及蛋白酶抑制剂活性对Zn胁迫的响应趋势不一致,体现为其营养物质含量减少、次生物质含量增多、蛋白酶抑制剂活性减弱。

     

  • 图  1  Zn胁迫对银中杨株高、根长和地径的影响

    图中数据均为平均值±标准差(n=3)。不同小写字母表示不同处理之间差异显著(P<0.05)。

    Figure  1.  Effects of Zn stress on plant height (A), root length (B) and ground diameter (C) of Populus alba 'berolinensis' seedlings

    Data in figure are means±SD (n=3). Different lowercases mean there is significant differences among the treatments at P<0.05 level.

    图  2  Zn胁迫对银中杨叶片内蛋白质(A)和可溶性糖(B)含量的影响

    图中数据均为平均值±标准差(n=3)。不同小写字母表示相同胁迫时间下各组之间差异显著(P<0.05)。下同。Data in figure are means±SD (n=3).

    Figure  2.  Effects of Zn stress on the protein (A) and soluble sugar (B) content in leaves of Populus alba 'berolinensis' seedlings

    Different lowercases within the same stress duration mean there is significant differences among the treatments at P<0.05 level. The same below.

    图  3  Zn胁迫对银中杨叶片内黄酮(A)和木质素(B)含量的影响

    Figure  3.  Effects of Zn stress on the flavonoids (A) and lignin (B) content in leaves of Populus alba 'berolinensis' seedlings

    图  4  Zn胁迫对银中杨叶片内TI和CI活性的影响

    Figure  4.  Effects of Zn stress on the trypsin inhibitor (TI) (A) and chymotrypsin inhibitor (CI) (B) activity in leaves of Populus alba 'berolinensis' seedlings

    表  1  Zn胁迫对银中杨根、茎、叶鲜质量和干质量的影响

    Table  1.   Effects of Zn stress on the fresh and dry mass of root, stem and leaf of Populus alba 'berolinensis' seedlings

    处理组Treatment根质量Root mass/(g·plant-1)茎质量Stem mass/(g· plant-1)叶质量Leaf mass/(g· plant-1)
    鲜质量Fresh mass干质量Dry mass鲜质量Fresh mass干质量Dry mass鲜质量Fresh mass干质量Dry mass
    CK4.98±0.59a3.77±0.29a14.93±0.81a7.58±0.51a19.54±2.01a9.09±0.96a
    Zn3003.92±0.19b3.06±0.34b13.66±0.19b6.73±0.1b16.77±0.15b7.02±0.18b
    Zn 5003.53±0.37b2.81±0.34b13.60±0.27b6.47±0.07b15.75±0.67b6.39±0.36b
    Zn 7003.46±0.33b2.64±0.28b11.24±1.33b5.26±0.67c15.53±0.53b6.36±0.3b
    注:表中数据均为平均值±标准差(n=3)。同列不同小写字母表示不同处理之间差异显著(P<0.05)。CK、Zn300、Zn500、Zn700分别代表土壤中的Zn处理含量为0、300、500和700 mg/kg。下同。Notes: data in table are means±SD (n=3). Different lowercases within the same column mean there is significant differences among the treatments at P<0.05 level. CK, Zn300, Zn500, Zn700 represent the Zn contents of 0, 300, 500, 700 mg/kg, respectively (the same below).
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