摘要:
为研究蜡梅SAMT 基因的调控功能,利用农杆菌介导法将其转入烟草中。经PCR 方法对转基因烟草进行检
测,结果显示:16 株转化植株中有15 株扩增出了目的条带,进一步的RT-PCR 检测结果表明,阳性植株均发生了正
确转录。对转基因烟草植株和未转基因对照植株进行植株大小、叶片形态、花色、花瓣大小以及花期进行观察,均
未发现有明显差异。采用顶空固相微萃取以及气相色谱-质谱技术(HS-SPME-GC-MS)对转基因烟草鲜花进行花
香成分分析,结果表明,转CpSAMT 基因烟草中有较高含量的苯甲醇、己烯醇、芳樟醇、石竹烯和苯甲醛等成分,但未
能检测到水杨酸甲酯和苯甲酸甲酯成分。
Abstract:
In order to investigate the biological function of Chimonanthus praecox SAMT gene, CpSAMT was
introduced into tobacco through Agrobacterium tumefaciens mediated transformation. Among 16
independent transgenic lines obtained, 15 lines were positive, which was revealed by PCR and further
confirmed by RT-PCR. Further analysis showed that transgenic plants were indistinguishable from
untransformed controls in terms of plant size, leaf morphology, flower color, petal size and flowering
time. In addition, HS-SPME-GC-MS was employed to analyse the components of flower flavor. We found
that the content of benzyl alcohol, hexenol, linalool, caryophyllene and benzaldehyde in transgenic
CpSAMT plants increased, while that of methyl salicylate and methyl benzoate was undetectable compared
with non-transgenic controls.
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