Influence of cultivating conditions on toxin produced by Pestalotiopsis funerea Desm
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摘要: 为进一步深入探索培养条件对枯斑拟盘多毛孢产毒能力的影响,对该菌在4种固体培养基与一种液体培养基上的产毒能力进行了比较研究.结果显示,枯斑拟盘多毛孢较适宜固体培养基的培养,能在麦粒、麦麸天然固体培养基上具有极强的产毒能力.在不同浓度毒素的生测反应中,上述两种培养基上产出的毒素在稀释20倍时,仍对松针(生测材料)有一定的致病活性.通过枯斑拟盘多毛孢代谢物提取组分的生测比较,发现该菌产出毒素溶于水,不易挥发.不同生测方式对毒素致病活性影响的研究显示,针刺法作用速度较快,但在低浓度毒素时,致病反应不如浸泡法明显.在此基础上,对枯斑拟盘多毛孢毒素作为除草剂作了一定研究,但致病效果不明显.一系列实验结果表明培养条件是该菌产毒能力大小的重要影响因素.Abstract: Influence of cultivating conditions on toxin production of Pestalotiopsis funerea was explored by the comparison of toxin produced from P.funerea cultivated on four solid media and a liquid culture medium.It was manifested that the growth of P.funerea on a solid medium was more suitable and its capacity of toxin production could reach very high when fungus grew on some natural solid media such as wheat and wheat-bran.The bio-determination results of different concentrations of toxin revealed that toxin produced in the two solid media mentioned above still had pathogenicity on needle leaves(bio-determination material) even being diluted 20 times.Toxin is dissolvable in water and difficult to volatilize in a bio-determination comparison of collected components from metabolic material of P.funerea.The effects of different methods of bio-determination on pathogenicity of toxin showed that the reaction speed of acupuncture was faster than water immersion.But the pathogenicity effect of acupuncture was less distinct than that of water immersion in low concentration toxin.It is indicated that the pathogenic effect on weeds was not significant in the experiment of utilizing P.funerea as herbicide.A series of experiments confirmed that cultivating conditions could significantly influence its capacity of toxin production.
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Keywords:
- Pestalotiopsis funerea /
- culture media /
- pine /
- toxin /
- bio-determination
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