高效液相色谱测定不同成熟期枣皮酚类物质

念红丽; 李赫; 曹冬冬; 曹建康; 姜微波

高效液相色谱测定不同成熟期枣皮酚类物质

1 College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, P. R. China; 2 Food Professional Division, Beijing Economics and Management School, 100142, P. R. China.

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出版历程
- 收稿日期: 1900-01-01
- 修回日期: 1900-01-01
- 刊出日期: 2011-01-30

Determination of phenolic compounds in jujube peels at different maturity stages by high performance liquid chromatography.

（1 中国农业大学食品科学与营养工程学院 2 北京市经济管理学校食品专业科）

摘要

摘要: 为进一步探讨成熟过程中酚类物质在枣果皮中的组成及分布情况，以冬枣为实验材料，利用高效液相色谱法分别对全绿、绿白、半红和全红4个成熟期的果皮中游离态、酯化态和结合态多酚进行了定性定量检测分析。色谱分析条件：色谱柱为WatersC18柱，流动相A泵为1%醋酸水、B泵为1%醋酸甲醇，总流速为0.8 mL/min，柱温30 ℃，进样量10 μL，检测波长280 nm。结果表明： 4个成熟期的冬枣果皮多酚都含有儿茶素、表儿茶素和香豆酸，并且儿茶素和表儿茶素主要以游离态和酯化态形式存在，而香豆酸在4个成熟期主要以酯化态和结合态形式存在。
- 高效液相色谱 /
- 枣皮 /
- 游离酚 /
- 酯化酚 /
- 结合酚
Abstract: This paper focus on the composition and distribution of phenolic compounds extracted from the peels of Chinese jujube (Ziziphus jujuba Mill. cv. Dongzao) fruits at various maturity stages. The free, ester and bound phenolic compounds were detected by the high performance liquid chromatography (HPLC) on a Waters C18 column. Gradient elution was employed with a mobile phase consisting of water-acetic acid (99∶1; A) and methanol-acetic acid (99∶1; B), a solvent flow rate of 0.8 mL/min and an injection volume of 10 μL. Phenolic compounds were identified at 280 nm. The results indicated that catechin, epicatechin and p-coumaric acid were the major phenolic compounds in the jujube peels at various maturity stages. The catechin and epicatechin were mainly free or in ester form. The p-coumaric acid was mainly in ester and bound forms in the peels of the jujube fruits.
- high performance liquid chromatography (HPLC) /
- jujube peel /
- free phenolic /
- ester phenolic /
- bound phenolic