毛白杨labA-like1基因的克隆与表达特性及其表达载体的构建
Isolation labA-like1 gene from Populus tomentosa: expression of PtlabA-like1 in P. tomentosa and construction of expression vector
-
摘要: 利用滤纸吸附噬菌体--PCR法，从毛白杨花芽cDNA文库中分离克隆了PtlabA-like1基因cDNA全长序列。 在Conserved Domains Database (CDD)中对该基因氨基酸保守结构域进行分析，发现其包含一个LabA-like superfamily结构域，故将其命名为PtlabA-like1。经BLAST分析，在毛果杨基因组数据库中检索到含有该保守结构域的其他9个家族成员。运用BioEdit和MEGA3.1软件，绘制了该基因家族表达蛋白的系统进化树。通过RT-PCR分析，揭示了该基因及其他家族成员在毛白杨中的组织差异表达模式：PtlabA-like1、PtlabA-like6呈现相似的组织表达模式，在茎、幼叶、成熟叶和雌雄花芽组织中均表达，但PtlabA-like1表达丰度较高；PtlabA-like8只在根部表达，且表达丰度较低；其他成员在根、茎、成熟叶、幼叶和雌雄花芽组织中均有表达，但是不同成员的表达丰度存在一定的差异。为进一步研究该基因的功能，构建了该基因的正义表达载体。Abstract: To understand the genetic and molecular mechanisms during floral development in Populus tomentosa, we isolated a full-length cDNA sequence of PtlabA-like1 gene from female floral bud cDNA library of P. tomentosa, with the method of bacteriophage absorption by filter paper-PCR. A conserved LabA-like superfamily domain was found via searching Conserved Domains Database. Therefore, it was given by the name PtlabA-like1. A phylogenetic tree was constructed using BioEdit and MEGA3.1 softwares to depict its evolutionary relationship with other 9 members comprising the same domain that retrieved via BLAST at JGI. The expression patterns of LabA-like superfamily members in tissues of P. tomentosa were estimated by RT-PCR. Results revealed that PtlabA-like1 and PtlabA-like6 had similar expression domains at stems, young leaves, mature leaves, male and female flower buds, but a higher abundance was especially shown in PtlabA-like1. PtlabA-like8 was detected with a low level at root only. Other members which had different abundances had a constitutive expression pattern at all of the six tissues. Furthermore, constitutive expression vector carrying 35S::PtlabA-like1 was constructed. All of these would provide the groundwork for understanding the function of PtlabA-like1 gene.
-
Keywords:
- Populus tomentosa /
- labA-like gene /
- cloning /
- expression
-
-
期刊类型引用(12)
1. 段泽伟，胡凌霞，毛巫甲，陈付康，鲁弘清. 甘洛县古树名木资源调查与保护对策. 中国林副特产. 2025(01): 73-75 . 
百度学术
2. 郑华英，刘云鹏，解春霞，徐丽丽，孟子翔. 江苏地区古树健康评价体系构建研究. 江苏林业科技. 2023(05): 17-23+52 . 百度学术
3. 晁永娟. 湟源县古树调查评价及保护对策研究. 宁夏农林科技. 2019(04): 41-42+45 . 百度学术
4. 李永良. 青海省乐都区古树名木资源调查与评价. 安徽农业科学. 2019(12): 132-133 . 百度学术
5. 张富强. 互助县古树名木资源调查及其保护对策. 陕西林业科技. 2019(03): 88-90 . 百度学术
6. 吴晓丽，李瑞军. 黔东南州古树名木的资源现状及其保护利用. 内蒙古林业调查设计. 2019(04): 42-45 . 百度学术
7. 张富强. 民和县古树名木资源调查评价及保护对策. 林业调查规划. 2019(05): 186-189 . 百度学术
8. 史生慧. 大通县古树调查评价及保护对策研究. 南方农业. 2019(26): 78-80 . 百度学术
9. 兰宇翔，乔雨轩，郭真真，余鹏飞，朱志鹏，董建文. 闽西闽东古树名木危险度权重评估. 长江大学学报(自科版). 2017(22): 15-18+3 . 百度学术
10. 肖瑾瑜，谭慧芳，陈俊友，汪爱民. 古树名木风险评估模型初步研究. 湖南林业科技. 2016(01): 61-65+79 . 百度学术
11. 朱志鹏，傅伟聪，陈梓茹，许春如，丁国昌，董建文. 闽东城乡古树危险度评估及保护措施——以闽侯县为例. 四川农业大学学报. 2015(04): 364-370 . 百度学术
12. 谢兴刚，石红旗. 城市生态环境中古树保护应对策略研究. 中国园艺文摘. 2015(10): 79-82 . 百度学术
其他类型引用(6)
计量
- 文章访问数: 6104
- HTML全文浏览量: 133
- PDF下载量: 51
- 被引次数: 18
下载:
